PMID- 37345512
OWN - NLM
STAT- Publisher
LR  - 20231013
IS  - 1526-4998 (Electronic)
IS  - 1526-498X (Linking)
VI  - 79
IP  - 11
DP  - 2023 Nov
TI  - Extrachromosomal DNA-mediated glyphosate resistance in Italian ryegrass.
PG  - 4290-4294
LID - 10.1002/ps.7626 [doi]
AB  - BACKGROUND: An Italian ryegrass population from Arkansas, USA developed 
      glyphosate resistance due to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) 
      gene amplification. The plants in this population with approximately 70 EPSPS 
      copies were used in the present study for the physical mapping of amplified 
      copies of EPSPS gene to determine the possible mechanism of EPSPS gene 
      amplification conferring glyphosate resistance in Italian ryegrass. RESULT: 
      Fluorescence in situ hybridization (FISH) analysis of glyphosate resistant (GR) 
      Italian ryegrass plants with approximately 70 EPSPS copies displayed EPSPS 
      hybridization signals randomly on most of the metaphase chromosomes. Glyphosate 
      susceptible (GS) Italian ryegrass plants with one EPSPS copy displayed single 
      prominent EPSPS hybridization signal, which was co-localized with 5S rDNA locus 
      along with few additional signals on the outside of chromosomes. Pulsed-field gel 
      electrophoresis (PFGE) followed by DNA blot using EPSPS gene as a probe 
      identified a prominent EPSPS hybridization around the 400 kb region in GR DNA 
      samples, but not in GS DNA samples. CONCLUSION: We report the extrachromosomal 
      DNA-mediated glyphosate resistance in Italian ryegrass. Physical mapping of 
      amplified copies of EPSPS gene in Italian ryegrass by FISH gives us a clue that 
      the amplified copies of EPSPS gene may be present in the extrachromosomal DNA 
      elements. Further analysis by PFGE followed by DNA blotting revealed that the 
      extrachromosomal DNA containing EPSPS is approximately 400 kb similar in size 
      with that of eccDNA replicon in Amaranthus palmeri. (c) 2023 Society of Chemical 
      Industry.
CI  - (c) 2023 Society of Chemical Industry.
FAU - Koo, Dal-Hoe
AU  - Koo DH
AUID- ORCID: 0000-0003-4578-8519
AD  - Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State 
      University, Manhattan, KS, USA.
FAU - Ju, Yoonha
AU  - Ju Y
AD  - Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State 
      University, Manhattan, KS, USA.
FAU - Putta, Karthik
AU  - Putta K
AD  - Department of Agronomy, Kansas State University, Manhattan, KS, USA.
FAU - Sathishraj, Rajendran
AU  - Sathishraj R
AD  - Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State 
      University, Manhattan, KS, USA.
FAU - Roma-Burgos, Nilda
AU  - Roma-Burgos N
AUID- ORCID: 0000-0002-6977-6873
AD  - Department of Crop, Soil and Environmental Sciences, University of Arkansas, 
      Fayetteville, AR, USA.
FAU - Jugulam, Mithila
AU  - Jugulam M
AUID- ORCID: 0000-0003-2065-9067
AD  - Department of Agronomy, Kansas State University, Manhattan, KS, USA.
FAU - Friebe, Bernd
AU  - Friebe B
AD  - Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State 
      University, Manhattan, KS, USA.
FAU - Gill, Bikram S
AU  - Gill BS
AD  - Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State 
      University, Manhattan, KS, USA.
LA  - eng
GR  - Wheat Genetics Resource Center Industry/ University Cooperative Research Center 
      National Science Foundation Contract 1822162/
PT  - Journal Article
DEP - 20230706
PL  - England
TA  - Pest Manag Sci
JT  - Pest management science
JID - 100898744
SB  - IM
OTO - NOTNLM
OT  - EPSPS gene amplification
OT  - FISH
OT  - extrachromosomal DNAs
OT  - glyphosate resistance
EDAT- 2023/06/22 06:42
MHDA- 2023/06/22 06:42
CRDT- 2023/06/22 04:33
PHST- 2023/05/25 00:00 [revised]
PHST- 2023/04/11 00:00 [received]
PHST- 2023/06/22 00:00 [accepted]
PHST- 2023/06/22 06:42 [pubmed]
PHST- 2023/06/22 06:42 [medline]
PHST- 2023/06/22 04:33 [entrez]
AID - 10.1002/ps.7626 [doi]
PST - ppublish
SO  - Pest Manag Sci. 2023 Nov;79(11):4290-4294. doi: 10.1002/ps.7626. Epub 2023 Jul 6.