PMID- 37346175
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230701
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 11
DP  - 2023
TI  - Inherent differences of small airway contraction and Ca(2+) oscillations in 
      airway smooth muscle cells between BALB/c and C57BL/6 mouse strains.
PG  - 1202573
LID - 10.3389/fcell.2023.1202573 [doi]
LID - 1202573
AB  - BALB/c and C57BL/6 mouse strains are widely used as animal model in studies of 
      respiratory diseases, such as asthma. Asthma is characterized by airway 
      hyperresponsiveness, which is eventually resulted from the excessive airway 
      smooth muscle (ASM) contraction mediated by Ca(2+) oscillations in ASM cells. It 
      is reported that BALB/c mice have inherently higher airway responsiveness, but 
      show no different contractive response of tracheal ring as compared to C57BL/6 
      mice. However, whether the different airway responsiveness is due to the 
      different extents of small airway contraction, and what's underlying mechanism 
      remains unknown. Here, we assess agonist-induced small airway contraction and 
      Ca(2+) oscillations in ASM cells between BALB/c and C57BL/6 mice by using 
      precision-cut lung slices (PCLS). We found that BALB/c mice showed an 
      intrinsically stronger extent of small airway narrowing and faster Ca(2+) 
      oscillations in ASM cells in response to agonists. These differences were 
      associated with a higher magnitude of Ca(2+) influx via store-operated Ca(2+) 
      entry (SOCE), as a result of increased expression of SOCE components (STIM1, 
      Orai1) in the ASM cells of small airway of BALB/c mice. An established 
      mathematical model and experimental results suggested that the increased SOC 
      current could result in increased agonist-induced Ca(2+) oscillations. Therefore, 
      the inherently higher SOC underlies the increased Ca(2+) oscillation frequency in 
      ASM cells and stronger small airway contraction in BALB/c mice, thus higher 
      airway responsiveness in BALB/c than C57BL/6 mouse strain.
CI  - Copyright (c) 2023 Zeng, Cheng, Li, Wang, Wen, Sanderson, Sneyd, Shen and Chen.
FAU - Zeng, Zijian
AU  - Zeng Z
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
FAU - Cheng, Mengxin
AU  - Cheng M
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
FAU - Li, Meng
AU  - Li M
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
FAU - Wang, Tao
AU  - Wang T
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
FAU - Wen, Fuqiang
AU  - Wen F
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
FAU - Sanderson, Michael J
AU  - Sanderson MJ
AD  - Department of Microbiology and Physiological Systems, University of Massachusetts 
      Medical School, Worcester, MA, United States.
FAU - Sneyd, James
AU  - Sneyd J
AD  - Department of Mathematics, The University of Auckland, Auckland, New Zealand.
FAU - Shen, Yongchun
AU  - Shen Y
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
FAU - Chen, Jun
AU  - Chen J
AD  - Department of Pulmonary and Critical Care Medicine, West China Hospital, Sichuan 
      University and Division of Pulmonary Diseases, State Key Laboratory of 
      Biotherapy, Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
DEP - 20230605
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC10279852
OTO - NOTNLM
OT  - Ca2+ oscillations
OT  - airway hyperresponsiveness
OT  - airway smooth muscle
OT  - asthma
OT  - precision-cut lung slices
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The handling editor, CM, declared a shared parent 
      affiliation with the authors ZZ, MC, ML, TW, FW, YS and JC at the time of review.
EDAT- 2023/06/22 13:09
MHDA- 2023/06/22 13:10
PMCR- 2023/01/01
CRDT- 2023/06/22 09:47
PHST- 2023/04/08 00:00 [received]
PHST- 2023/05/26 00:00 [accepted]
PHST- 2023/06/22 13:10 [medline]
PHST- 2023/06/22 13:09 [pubmed]
PHST- 2023/06/22 09:47 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1202573 [pii]
AID - 10.3389/fcell.2023.1202573 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2023 Jun 5;11:1202573. doi: 10.3389/fcell.2023.1202573. 
      eCollection 2023.