PMID- 37346847
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 0972-124X (Print)
IS  - 0975-1580 (Electronic)
IS  - 0972-124X (Linking)
VI  - 27
IP  - 3
DP  - 2023 May-Jun
TI  - "Efficacy of cytotoxic effect of green tea catechins on the human periodontal 
      fibroblasts and human dental pulp fibroblasts -An in vitro study".
PG  - 273-277
LID - 10.4103/jisp.jisp_168_22 [doi]
AB  - BACKGROUND: Inflammation of tooth-supporting tissue and the pulp tissue is 
      followed by wound healing and regeneration process that involves the specific 
      type of connective tissue cells, the fibroblasts. During periodontitis and 
      pulpitis, the inflammation of the tissue causes damage to the fibroblasts. These 
      fibroblasts secrete collagen proteins and maintain the structural framework; 
      along with this the inflammatory process moves toward healing where in the 
      specific cells such as the fibroblast cells play important roles. Green tea 
      catechins epigallocatechin-3-gallate (EGCG) being one of the major catechins is 
      known to have multiple beneficial effects on human fibroblasts. OBJECTIVE: To 
      assess the in vitro cytotoxicity of green tea catechins on the human periodontal 
      ligament (PDL) fibroblasts and human dental pulp fibroblasts. MATERIALS AND 
      METHODS: Human PDL fibroblasts (hPDLFs) and human dental pulp fibroblasts were 
      isolated from the two extracted premolar teeth that were indicated for 
      orthodontic treatment. The fibroblasts were then seeded in 96 well tissue culture 
      plate for cell viability study. EGCG was used at different concentration to treat 
      the cells. After 48 h; (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium 
      Bromide) (MTT) assay was performed to determine the cell viability. RESULTS: The 
      vitality of hPDLFs and human dental pulp fibroblasts was found to be inversely 
      proportional to EGCG concentrations. CONCLUSIONS: hPDLFs have shown 37% 
      proliferation at lowest concentration of EGCG used and human dental pulp 
      fibroblasts have shown 99% viability at lowest concentration of EGCG used.
CI  - Copyright: (c) 2023 Indian Society of Periodontology.
FAU - Hattarki, Sanjeevini
AU  - Hattarki S
AD  - Department of Periodontology, Maratha Mandal's Ngh Institute of Dental Sciences 
      and Research Centre, Belagavi, Karnataka, India.
FAU - Bogar, Chetana
AU  - Bogar C
AD  - Department of Central Research Laboratory, Maratha Mandal's Ngh Institute of 
      Dental Sciences and Research Centre, Belagavi, Karnataka, India.
FAU - Bhat, Kishore
AU  - Bhat K
AD  - Department of Central Research Laboratory, Maratha Mandal's Ngh Institute of 
      Dental Sciences and Research Centre, Belagavi, Karnataka, India.
LA  - eng
PT  - Journal Article
DEP - 20230501
PL  - India
TA  - J Indian Soc Periodontol
JT  - Journal of Indian Society of Periodontology
JID - 101499342
PMC - PMC10281319
OTO - NOTNLM
OT  - Dental pulp fibroblast
OT  - epigallocatechin-3-gallate
OT  - green tea catechins
OT  - human gingival fibroblast
OT  - periodontal disease
COIS- There are no conflicts of interest.
EDAT- 2023/06/22 13:09
MHDA- 2023/06/22 13:10
PMCR- 2023/05/01
CRDT- 2023/06/22 09:56
PHST- 2022/04/04 00:00 [received]
PHST- 2022/11/16 00:00 [revised]
PHST- 2022/11/27 00:00 [accepted]
PHST- 2023/06/22 13:10 [medline]
PHST- 2023/06/22 13:09 [pubmed]
PHST- 2023/06/22 09:56 [entrez]
PHST- 2023/05/01 00:00 [pmc-release]
AID - JISP-27-273 [pii]
AID - 10.4103/jisp.jisp_168_22 [doi]
PST - ppublish
SO  - J Indian Soc Periodontol. 2023 May-Jun;27(3):273-277. doi: 
      10.4103/jisp.jisp_168_22. Epub 2023 May 1.