PMID- 37351102 OWN - NLM STAT- MEDLINE DCOM- 20230626 LR - 20240227 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 14 DP - 2023 TI - Biochemical association of regulatory variant of KLF14 genotype in the pathogenesis of cardiodiabetic patients. PG - 1176166 LID - 10.3389/fendo.2023.1176166 [doi] LID - 1176166 AB - BACKGROUND AND PURPOSE: The study focuses on examining the relationship between a single nucleotide polymorphism (SNP) in KLF14 rs4731702 and risk of type 2 diabetes mellitus (T2DM) and dyslipidemia in different ethnic populations. The purpose of this study was to evaluate the association between KLF14 rs4731702 and serum lipid profile and to determine the frequency distribution of KLF14 rs4731702 among T2DM and cardiometabolic patients. METHODS: A total of 300 volunteers were recruited, consisting of three groups: 100 healthy individuals, 100 individuals diagnosed with T2DM, and 100 individuals diagnosed with cardiometabolic disorders. Biochemical analysis of blood samples was conducted to assess various biomarkers related to glycemic control and lipid profile. This involved measuring levels of glucose, triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and ApoA1. Genotyping analysis was performed to investigate KLF14 rs4731702 polymorphism. The Tetra ARMS-PCR method was employed for genotyping analysis. RESULTS: The results of biochemical profiling revealed a significant association between altered glycemic biomarkers and lipid profile in diseased patients compared to healthy participants. The frequencies of KLF14 rs4731702 alleles and genotypes were compared between the control group and T2DM group. A statistically significant difference was observed, indicating a potential association between KLF14 rs4731702 and T2DM. In the dominant inheritance model of KLF14 rs4731702 SNP, a statistically significant difference [odds ratio (95% confidence interval)] of 0.56 (0.34 -0.96) was found between the control and T2DM subjects. This suggests that the presence of certain genotypes influences the risk of T2DM. In T2DM patients, individuals carrying the C allele exhibited compromised insulin sensitivity, decreased HDL-C and ApoA1 levels, and increased serum glucose, TG, and LDL-C concentrations. Conversely, TT genotype carriers demonstrated increased levels of HDL-C and ApoA1, lower insulin resistance, serum glucose, LDL-C, and TG levels. CONCLUSION: The study's findings indicate that dyslipidemia in T2DM patients is associated with reduced KLF14 functionality due to CC and CT genotypes, leading to insulin resistance and an increased risk of cardiovascular diseases. Additionally, risk of KLF14 rs4731702 polymorphism was found to increase with age and was more prevalent in female than in male individuals. These insights contribute to understanding genetic factors influencing the development and progression of T2DM and dyslipidemia in different ethnic populations. CI - Copyright (c) 2023 Alanazi, Rasheed, Rehman, Mallhi, Akash, Alotaibi, Alzarea, Tanveer and Khan. FAU - Alanazi, Abdullah Salah AU - Alanazi AS AD - Department of Clinical Pharmacy, College of Pharmacy, Jouf University, Sakaka, Al-Jouf, Saudi Arabia. AD - Health Sciences Research Unit, Jouf University, Sakaka, Al-Jouf, Saudi Arabia. FAU - Rasheed, Sumbal AU - Rasheed S AD - Department of Pharmaceutical Chemistry, Government College University, Faisalabad, Pakistan. FAU - Rehman, Kanwal AU - Rehman K AD - Department of Pharmacy, The Women University, Multan, Pakistan. FAU - Mallhi, Tauqeer Hussain AU - Mallhi TH AD - Department of Clinical Pharmacy, College of Pharmacy, Jouf University, Sakaka, Al-Jouf, Saudi Arabia. FAU - Akash, Muhammad Sajid Hamid AU - Akash MSH AD - Department of Pharmaceutical Chemistry, Government College University, Faisalabad, Pakistan. FAU - Alotaibi, Nasser Hadal AU - Alotaibi NH AD - Department of Clinical Pharmacy, College of Pharmacy, Jouf University, Sakaka, Al-Jouf, Saudi Arabia. FAU - Alzarea, Abdulaziz Ibrahim AU - Alzarea AI AD - Department of Clinical Pharmacy, College of Pharmacy, Jouf University, Sakaka, Al-Jouf, Saudi Arabia. FAU - Tanveer, Nida AU - Tanveer N AD - Institute of Molecular Cardiology, University of Louisville, Louisville, KY, United States. FAU - Khan, Yusra Habib AU - Khan YH AD - Department of Clinical Pharmacy, College of Pharmacy, Jouf University, Sakaka, Al-Jouf, Saudi Arabia. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230607 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 0 (Cholesterol, LDL) RN - 0 (Lipids) RN - 0 (Triglycerides) RN - 0 (Cholesterol, HDL) RN - 0 (Biomarkers) RN - IY9XDZ35W2 (Glucose) RN - 0 (KLF14 protein, human) RN - 0 (Kruppel-Like Transcription Factors) SB - IM MH - Humans MH - Male MH - Female MH - *Diabetes Mellitus, Type 2/genetics MH - Cholesterol, LDL MH - *Insulin Resistance MH - Lipids MH - Gene Frequency MH - Genotype MH - Triglycerides MH - Cholesterol, HDL MH - Biomarkers MH - Glucose MH - *Dyslipidemias/genetics MH - Kruppel-Like Transcription Factors/genetics PMC - PMC10282989 OTO - NOTNLM OT - KLF14 rs4731702 OT - Tetra-ARMS-PCR OT - diabetes mellitus OT - dyslipidemia OT - genotypic analysis OT - risk factors OT - single nucleotide polymorphism COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/06/23 13:07 MHDA- 2023/06/26 06:41 PMCR- 2023/01/01 CRDT- 2023/06/23 10:29 PHST- 2023/02/28 00:00 [received] PHST- 2023/05/18 00:00 [accepted] PHST- 2023/06/26 06:41 [medline] PHST- 2023/06/23 13:07 [pubmed] PHST- 2023/06/23 10:29 [entrez] PHST- 2023/01/01 00:00 [pmc-release] AID - 10.3389/fendo.2023.1176166 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2023 Jun 7;14:1176166. doi: 10.3389/fendo.2023.1176166. eCollection 2023.