PMID- 37351958
OWN - NLM
STAT- MEDLINE
DCOM- 20230626
LR  - 20230626
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 43
IP  - 7
DP  - 2023 Jul
TI  - Clinical Significance of Nodal DCsign Expression in Non-small-cell Lung Cancer 
      Patients.
PG  - 3003-3013
LID - 10.21873/anticanres.16471 [doi]
AB  - BACKGROUND/AIM: Dendritic cells (DCs) are difficult to evaluate in lung regional 
      lymph nodes because of region-specific structures, such as abundant trabeculae 
      connecting the medullary and subcapsular sinuses, the latter of which contains 
      few anthracotic macrophages. Therefore, DC-specific intercellular adhesion 
      molecule-3-grabbing non-integrin (DCsign)-positive DCs and CD68-positive 
      macrophages are unlikely to show a typical distribution. The present study 
      therefore explored quantitative factors connecting the nodal DC morphology to the 
      patient outcome. MATERIALS AND METHODS: Lymph nodes from 34 non-small-cell lung 
      cancer patients who underwent complete resection were used for 
      immunohistochemical assessments of DCsign and CD68 and terminal deoxynucleotidyl 
      transferase dUTP nick-end labeling. Preoperative patient blood samples were used 
      for the quantitative evaluation of monocytes. RESULTS: The nodal DCs showed a 
      complementary distribution with macrophages, thus few DCs were seen in clusters 
      of macrophages. DCs often presented as a mesh-like rosette that was solitary or 
      connected to a DC cluster. DCs disappeared, and some macrophages were apoptotic 
      when surrounded by cancer cells that have metastasized to lymph nodes. The 
      proportional area of a DC cluster was significantly associated with the 
      histological differentiation of cancer (p=0.013), with a higher ratio tending to 
      lead to a better overall survival (p=0.059), and significantly so in 
      adenocarcinoma (p=0.007). The rosette number was significantly correlated with 
      the smoking index and blood monocyte number (p=0.013 and p=0.005, respectively). 
      CONCLUSION: The nodal DC morphology appears useful as a prognostic factor and may 
      lead to a new phase of clinicopathological studies of solid cancers.
CI  - Copyright (c) 2023 International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Aoki, Masaya
AU  - Aoki M
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan; mtytah5046@gmail.com.
FAU - Kamimura, G O
AU  - Kamimura GO
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Morizono, Shoichiro
AU  - Morizono S
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Tokunaga, Takuya
AU  - Tokunaga T
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Umehara, Tadashi
AU  - Umehara T
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Harada-Takeda, Aya
AU  - Harada-Takeda A
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Maeda, Koki
AU  - Maeda K
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Nagata, Toshiyuki
AU  - Nagata T
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Ueda, Kazuhiro
AU  - Ueda K
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
FAU - Tabata, Kazuhiro
AU  - Tabata K
AD  - Department of Pathology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
FAU - Murakami, Gen
AU  - Murakami G
AD  - Department of Anatomy, Tokyo Dental College, Tokyo, Japan.
FAU - Sato, Masami
AU  - Sato M
AD  - Department of General Thoracic Surgery, Kagoshima University Graduate School of 
      Medical and Dental Sciences, Kagoshima, Japan.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Humans
MH  - *Carcinoma, Non-Small-Cell Lung/pathology
MH  - *Lung Neoplasms/pathology
MH  - Clinical Relevance
MH  - Macrophages/metabolism
MH  - Monocytes/pathology
MH  - Lymph Nodes/pathology
MH  - Transforming Growth Factor beta/metabolism
MH  - Dendritic Cells/metabolism
OTO - NOTNLM
OT  - DCsign
OT  - Dendritic cell
OT  - clinicopathological factors
OT  - lymph node
OT  - macrophage
OT  - non-small-cell lung cancer
OT  - prognosis
EDAT- 2023/06/23 19:11
MHDA- 2023/06/26 06:41
CRDT- 2023/06/23 12:13
PHST- 2023/04/10 00:00 [received]
PHST- 2023/05/13 00:00 [revised]
PHST- 2023/05/15 00:00 [accepted]
PHST- 2023/06/26 06:41 [medline]
PHST- 2023/06/23 19:11 [pubmed]
PHST- 2023/06/23 12:13 [entrez]
AID - 43/7/3003 [pii]
AID - 10.21873/anticanres.16471 [doi]
PST - ppublish
SO  - Anticancer Res. 2023 Jul;43(7):3003-3013. doi: 10.21873/anticanres.16471.