PMID- 37353739
OWN - NLM
STAT- MEDLINE
DCOM- 20230626
LR  - 20230701
IS  - 1471-2415 (Electronic)
IS  - 1471-2415 (Linking)
VI  - 23
IP  - 1
DP  - 2023 Jun 23
TI  - Knockout of PERK protects rat Muller glial cells against OGD-induced endoplasmic 
      reticulum stress-related apoptosis.
PG  - 286
LID - 10.1186/s12886-023-03022-z [doi]
LID - 286
AB  - BACKGROUND: The pathological basis for many retinal diseases, retinal ischemia is 
      also one of the most common causes of visual impairment. Numerous ocular diseases 
      have been linked to Endoplasmic reticulum(ER)stress. However, there is still no 
      clear understanding of the relationship between ER stress and Muller glial cells 
      during retinal ischemia and hypoxia. This study examined the effects of ER stress 
      on autophagy and apoptosis-related proteins, as well as the microtubule-related 
      protein tau in rMC-1 cells. METHODS: rMC-1 cells were cultured in vitro. 
      RT-PCR、immunofluorescence and Western blotting revealed the expression levels of 
      associated mRNAs and proteins, and the CCK-8 and flow cytometry assays detected 
      cell apoptosis. RESULTS: The results showed that under OGD(Oxygen-glucose 
      deprivation) conditions, the number of rMC-1 cells was decreased, the PERK/eIF2a 
      pathway was activated, and the expressions of p-tau, LC3、Beclin1 and Caspase-12 
      proteins were increased. After the PERK knockout, the expression of the above 
      proteins was decreased, and the apoptosis was also decreased. CONCLUSION: 
      According to the findings of this study, specific downregulation of PERK 
      expression had an anti-apoptotic effect on OGD-conditioned rMC-1 cells. There is 
      a possibility that this is one of the mechanisms of MG cell apoptosis during 
      retinal ischemic injury.
CI  - (c) 2023. The Author(s).
FAU - Wang, Xiaorui
AU  - Wang X
AD  - Department of Ophthalmology, Second Affiliated Hospital of Fujian Medical 
      University, No.950, Donghai Street, Quanzhou, 362000, Fujian Province, China.
FAU - Zhu, Xinxing
AU  - Zhu X
AD  - Department of Ophthalmology, Second Affiliated Hospital of Fujian Medical 
      University, No.950, Donghai Street, Quanzhou, 362000, Fujian Province, China.
FAU - Huang, Guangqian
AU  - Huang G
AD  - Department of Ophthalmology, Second Affiliated Hospital of Fujian Medical 
      University, No.950, Donghai Street, Quanzhou, 362000, Fujian Province, China.
FAU - Wu, Lili
AU  - Wu L
AD  - Department of Ophthalmology, Second Affiliated Hospital of Fujian Medical 
      University, No.950, Donghai Street, Quanzhou, 362000, Fujian Province, China.
FAU - Meng, Zhiyong
AU  - Meng Z
AD  - Department of Ophthalmology, Second Affiliated Hospital of Fujian Medical 
      University, No.950, Donghai Street, Quanzhou, 362000, Fujian Province, China.
FAU - Wu, Yuyu
AU  - Wu Y
AD  - Department of Ophthalmology, Second Affiliated Hospital of Fujian Medical 
      University, No.950, Donghai Street, Quanzhou, 362000, Fujian Province, China. 
      qzwyydoctor@163.com.
LA  - eng
GR  - 2021C042R/Quanzhou Program of High-level Talents Innovation Entrepreneurship/
GR  - 2020CXA043/Provincial medical innovation project in Fujian Province/
GR  - [2022]884/Key Clinical Specialty Discipline Construction Program of Fujian，P.R.C/
PT  - Journal Article
DEP - 20230623
PL  - England
TA  - BMC Ophthalmol
JT  - BMC ophthalmology
JID - 100967802
SB  - IM
MH  - Rats
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - *Signal Transduction
MH  - *Ependymoglial Cells
MH  - Apoptosis
MH  - Endoplasmic Reticulum Stress
PMC - PMC10290337
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Endoplasmic reticulum stress
OT  - Muller glia
OT  - Oxygen-glucose deprivation
COIS- The authors declare no competing interests.
EDAT- 2023/06/24 11:42
MHDA- 2023/06/26 06:42
PMCR- 2023/06/23
CRDT- 2023/06/23 23:31
PHST- 2022/10/31 00:00 [received]
PHST- 2023/06/06 00:00 [accepted]
PHST- 2023/06/26 06:42 [medline]
PHST- 2023/06/24 11:42 [pubmed]
PHST- 2023/06/23 23:31 [entrez]
PHST- 2023/06/23 00:00 [pmc-release]
AID - 10.1186/s12886-023-03022-z [pii]
AID - 3022 [pii]
AID - 10.1186/s12886-023-03022-z [doi]
PST - epublish
SO  - BMC Ophthalmol. 2023 Jun 23;23(1):286. doi: 10.1186/s12886-023-03022-z.