PMID- 37355557
OWN - NLM
STAT- MEDLINE
DCOM- 20230626
LR  - 20231117
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2691
DP  - 2023
TI  - Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation.
PG  - 351-369
LID - 10.1007/978-1-0716-3331-1_25 [doi]
AB  - Epitope-specific immunotherapies have enabled the targeted treatment of a variety 
      of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8(+) 
      T cell-based therapies, the precise identification of immunogenic peptides 
      presented by human leukocyte antigen (HLA) class I is essential which can be 
      achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and 
      cell sorting approaches, we present a method to engineer a cell line that does 
      not express its native HLA but instead expresses an HLA of interest (in this 
      instance HLA-A*02:01). This technique can be used to elucidate the 
      immunopeptidome of cell lines expressing different HLAs.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Jin, Dongbin
AU  - Jin D
AD  - Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, 
      VIC, Australia.
FAU - Loh, Khai Lee
AU  - Loh KL
AD  - Centre for Inflammatory Diseases, Department of Medicine, Monash University, 
      Monash Medical Centre, Clayton, VIC, Australia.
FAU - Shamekhi, Tima
AU  - Shamekhi T
AD  - Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, 
      VIC, Australia.
FAU - Ting, Yi Tian
AU  - Ting YT
AD  - Centre for Inflammatory Diseases, Department of Medicine, Monash University, 
      Monash Medical Centre, Clayton, VIC, Australia.
FAU - Lim Kam Sian, Terry C C
AU  - Lim Kam Sian TCC
AD  - Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, 
      VIC, Australia.
AD  - Monash Proteomics & Metabolomics Facility, Department of Biochemistry and 
      Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, 
      Clayton, VIC, Australia.
FAU - Roest, James
AU  - Roest J
AD  - St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.
FAU - Ooi, Joshua D
AU  - Ooi JD
AD  - Centre for Inflammatory Diseases, Department of Medicine, Monash University, 
      Monash Medical Centre, Clayton, VIC, Australia.
FAU - Vivian, Julian P
AU  - Vivian JP
AD  - St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia. 
      jvivian@svi.edu.au.
AD  - Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia. 
      jvivian@svi.edu.au.
FAU - Faridi, Pouya
AU  - Faridi P
AD  - Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, 
      VIC, Australia.
AD  - Monash Proteomics & Metabolomics Facility, Department of Biochemistry and 
      Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, 
      Clayton, VIC, Australia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Epitopes, T-Lymphocyte)
SB  - IM
MH  - Humans
MH  - *Antigens, Neoplasm
MH  - *Histocompatibility Antigens Class I/genetics
MH  - HLA Antigens/genetics
MH  - Histocompatibility Antigens Class II
MH  - Cell Line, Tumor
MH  - Epitopes, T-Lymphocyte
MH  - Antigen Presentation
OTO - NOTNLM
OT  - Cancer
OT  - Cell lines
OT  - Gene editing
OT  - HLA
OT  - Immunopeptidomics
OT  - Immunotherapy
OT  - Lentivirus
OT  - Neoantigens
OT  - Transduction
EDAT- 2023/06/25 01:08
MHDA- 2023/06/26 06:41
CRDT- 2023/06/24 23:05
PHST- 2023/06/26 06:41 [medline]
PHST- 2023/06/25 01:08 [pubmed]
PHST- 2023/06/24 23:05 [entrez]
AID - 10.1007/978-1-0716-3331-1_25 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2691:351-369. doi: 10.1007/978-1-0716-3331-1_25.