PMID- 37356951
OWN - NLM
STAT- MEDLINE
DCOM- 20230627
LR  - 20230627
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 31
IP  - 3
DP  - 2023 Jun
TI  - [Analysis of Gene Recombination between HLA-B and -DRB1, HLA-DQB1 and -DPB1 
      Loci].
PG  - 855-859
LID - 10.19746/j.cnki.issn.1009-2137.2023.03.035 [doi]
AB  - OBJECTIVE: To investigate the recombinations within the human leukocyte antigen 
      (HLA) region in two families. METHODS: Genomic DNA was extracted from the 
      peripheral blood specimens of the different family members. HLA-A, -B, -C, -DRB1, 
      -DQB1 and -DPB1 loci were genotyped using polymerase chain reaction-sequence 
      specific oligonucleotide probing technique (PCR-SSO) and next-generation 
      sequencing technique. HLA haplotype was determined by genetic analysis of the 
      pedigree. RESULTS: The haplotypes of 
      HLA-A*11:01~C*03:04~B*13:01~DRB1*12:02~DQB1*03:01~DPB1*05:01:01G and 
      HLA-A*03:01~C*04:01~B*35:03~DRB1*12:01~DQB1*03:01~DPB1*04:01:01G in the family 1 
      were recombined between HLA-B and HLA-DRB1 loci, which formed the haplotype of 
      HLA-A*11:01~C*03:04~B*13:01~DRB1* 12:01~DQB1*03:01~DPB1*04:01:01G. The haplotypes 
      of HLA-A *02:06~C*03:03~B*35:01~DRB1*08:02~DQB1*04:02~ DPB1*13:01:01G and HLA-A 
      *11:01~C*07:02~B*38:02~DRB1*15:02~DQB1*05:01~DPB1*05:01:01G in the family 2 were 
      recombined between HLA-DQB1 and HLA-DPB1 loci, which formed the haplotype of 
      HLA-A*02:06~C*03:03~B*35:01~ DRB1*08:02~DQB1*04:02~DPB1*05:01:01G. CONCLUSION: 
      The gene recombination events between HLA-B and -DRB1, HLA-DQB1 and -DPB1 loci 
      were found respectively in two Chinese Han families.
FAU - Chen, Chen
AU  - Chen C
AD  - School of Medical Technology and Information Engineering, Zhejiang Chinese 
      Medical University, Hangzhou 310053, Zhejiang Province, China; Blood Center of 
      Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key Laboratory of 
      Blood Safety Research of Zhejiang Province, Hangzhou 310052, Zhejiang Province, 
      China.
FAU - Wang, Wei
AU  - Wang W
AD  - Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key 
      Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, 
      Zhejiang Province, China.
FAU - Chen, Nan-Ying
AU  - Chen NY
AD  - Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key 
      Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, 
      Zhejiang Province, China.
FAU - Dong, Li-Na
AU  - Dong LN
AD  - Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key 
      Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, 
      Zhejiang Province, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key 
      Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, 
      Zhejiang Province, China.
FAU - Zhu, Fa-Ming
AU  - Zhu FM
AD  - Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key 
      Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, 
      Zhejiang Province, China.E-mail: zfm00@hotmail.com.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Humans
MH  - Gene Frequency
MH  - HLA-DQ beta-Chains/genetics
MH  - *HLA-B Antigens/genetics
MH  - *Histocompatibility Antigens Class I/genetics
MH  - Haplotypes
MH  - HLA-A Antigens/genetics
MH  - HLA-DRB1 Chains/genetics
MH  - Recombination, Genetic
MH  - Alleles
OTO - NOTNLM
OT  - HLA-B and HLA-DRB1 loci
OT  - HLA-DQB1 and HLA-DPB1 loci
OT  - gene recombination
OT  - human leukocyte antigen (HLA)
OT  - next-generation sequencing
EDAT- 2023/06/26 00:41
MHDA- 2023/06/27 06:42
CRDT- 2023/06/25 21:03
PHST- 2023/06/27 06:42 [medline]
PHST- 2023/06/26 00:41 [pubmed]
PHST- 2023/06/25 21:03 [entrez]
AID - 1009-2137(2023)03-855-05 [pii]
AID - 10.19746/j.cnki.issn.1009-2137.2023.03.035 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2023 Jun;31(3):855-859. doi: 
      10.19746/j.cnki.issn.1009-2137.2023.03.035.