PMID- 37357556
OWN - NLM
STAT- MEDLINE
DCOM- 20230817
LR  - 20230907
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 67
IP  - 16
DP  - 2023 Aug
TI  - Milk Exosomes from Gestational Diabetes Mellitus (GDM) and Healthy Parturient 
      Exhibit Differential miRNAs Profiles and Distinct Regulatory Bioactivity on 
      Hepatocyte Proliferation.
PG  - e2300005
LID - 10.1002/mnfr.202300005 [doi]
AB  - SCOPE: Exosomes, a novel type of bioactive component in human milk (HM), affect 
      infant development, growth, and health. Recent studies indicate that HM exosomes 
      and miRNAs relate to gestational diabetes mellitus (GDM). However, the miRNAs 
      profiles and functionalities of HM exosomes from GDM parturient remain unclear. 
      This study aims to compare the differential miRNAs in HM exosomes from GDM and 
      healthy parturient, and investigate the HM exosomes bioactivities in regulating 
      hepatocyte proliferation and insulin sensitivity. METHODS AND RESULTS: This study 
      extracted HM exosomes from GDM (GDM-EXO) and healthy (NOR-EXO) parturient by 
      ultracentrifugation, high-throughput sequenced and compared the exosomal miRNAs 
      profiles, and explored the regulatory bioactivities on hepatocyte proliferation 
      in HepG2 cells and Balb/c mice. As compared to NOR-EXO, GDM-EXO has similar 
      morphology, size, concentration, and exosome-specific markers (CD9 and TSG101) 
      expression. GDM-EXO and NOR-EXO specifically harbor 1299 and 8 miRNAs, 
      respectively. Moreover, GDM-EXO had 176 upregulated and 47 downregulated miRNAs 
      compared with NOR-EXO. Both GDM-EXO and NOR-EXO were absorbed in cultured HepG2 
      hepatocytes and mice liver. GDM-EXO inhibited hepatocytes proliferation by 
      downregulating mammalian target of rapamycin (mTOR) possibly via exosomal 
      miR-101-3p delivery. CONCLUSION: HM exosomes from GDM and healthy parturient 
      exhibit differential miRNAs profiles and distinct regulatory bioactivity on 
      hepatocyte proliferation.
CI  - (c) 2023 Wiley-VCH GmbH.
FAU - Zheng, Zhongdaixi
AU  - Zheng Z
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
AD  - Department of Environmental Health, School of Public Health, National Medical 
      Products Administration (NMPA) Key Laboratory for Safety Evaluation of Cosmetics, 
      Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern 
      Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Mo, Jiaqi
AU  - Mo J
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Lin, Fengjuan
AU  - Lin F
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Wang, Jiexian
AU  - Wang J
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Chen, Junbin
AU  - Chen J
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Luo, Huiyu
AU  - Luo H
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Liu, Yuguo
AU  - Liu Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Su, Chuhong
AU  - Su C
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Gu, Xiangfu
AU  - Gu X
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Xiong, Fei
AU  - Xiong F
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
FAU - Zha, Longying
AU  - Zha L
AUID- ORCID: 0000-0002-2648-2162
AD  - Department of Nutrition and Food Hygiene, School of Public Health, National 
      Medical Products Administration (NMPA) Key Laboratory for Safety Evaluation of 
      Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, 
      Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230625
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Pregnancy
MH  - Female
MH  - Animals
MH  - Mice
MH  - Child
MH  - Humans
MH  - *Diabetes, Gestational/genetics
MH  - Milk
MH  - *Exosomes/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Hepatocytes/metabolism
MH  - Cell Proliferation
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - exosomes
OT  - gestational diabetes mellitus
OT  - hepatocyte proliferation
OT  - human milk
OT  - miRNAs
EDAT- 2023/06/26 06:41
MHDA- 2023/08/17 06:42
CRDT- 2023/06/26 03:09
PHST- 2023/03/27 00:00 [revised]
PHST- 2023/01/05 00:00 [received]
PHST- 2023/08/17 06:42 [medline]
PHST- 2023/06/26 06:41 [pubmed]
PHST- 2023/06/26 03:09 [entrez]
AID - 10.1002/mnfr.202300005 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2023 Aug;67(16):e2300005. doi: 10.1002/mnfr.202300005. Epub 
      2023 Jun 25.