PMID- 37360245
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230701
IS  - 2169-7574 (Print)
IS  - 2169-7574 (Electronic)
IS  - 2169-7574 (Linking)
VI  - 11
IP  - 5
DP  - 2023 May
TI  - Fluorescence In Situ Hybridization as Diagnostic Tool for Implant-associated 
      Infections: A Pilot Study on Added Value.
PG  - e4994
LID - 10.1097/GOX.0000000000004994 [doi]
LID - e4994
AB  - Implant-associated infections are a devastating complication in surgery. 
      Especially in infections with biofilm-forming microorganisms, the identification 
      of the causing microorganism remains a challenge. However, the classification as 
      biofilm is not possible with conventional polymerase chain reaction or 
      culture-based diagnostics. The aim of this study was to evaluate the additional 
      value of fluorescence in situ hybridization (FISH) and nucleic acid amplification 
      technique (FISHseq) to discuss a diagnostic benefit of the culture-independent 
      methods and to map spatial organization of pathogens and microbial biofilms in 
      wounds. METHODS: In total, 118 tissue samples from 60 patients with clinically 
      suspected implant-associated infections (n = 32 joint replacements, n = 24 open 
      reduction and internal fixation, n = 4 projectiles) were analyzed using classic 
      microbiological culture and culture-independent FISH in combination with 
      polymerase chain reaction and sequencing (FISHseq). RESULTS: In 56 of 60 wounds, 
      FISHseq achieved an added value. FISHseq confirmed the result of cultural 
      microbiological examinations in 41 of the 60 wounds. In 12 wounds, one or more 
      additional pathogens were detected by FISHseq. FISHseq could show that the 
      bacteria initially detected by culture corresponded to a contamination in three 
      wounds and could exclude that the identified commensal pathogens were a 
      contamination in four other wounds. In five wounds, a nonplanktonic bacterial 
      life form was detected. CONCLUSIONS: The study revealed that FISHseq gives 
      additional diagnostic information, including therapy-relevant findings that were 
      missed by culture. In addition, nonplanktonic bacterial life forms could also be 
      detected with FISHseq, albeit less frequently than previously indicated.
CI  - Copyright (c) 2023 The Authors. Published by Wolters Kluwer Health, Inc. on behalf 
      of The American Society of Plastic Surgeons.
FAU - Scheuermann-Poley, Catharina
AU  - Scheuermann-Poley C
AD  - From the Trauma & Orthopedic Surgery, Septic & Reconstructive Surgery, Research 
      and Treatment Centre Septic Defect Wounds, Federal Armed Forces of Germany, 
      Bundeswehr (Military) Academic Hospital, Berlin, Germany.
FAU - Wiessner, Alexandra
AU  - Wiessner A
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases, and Immunology, 
      Charite - University Medicine Berlin and MoKi Analytics GmbH, Berlin, Germany.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases, and Immunology, 
      Charite - University Medicine Berlin and MoKi Analytics GmbH, Berlin, Germany.
FAU - Gatzer, Renate
AU  - Gatzer R
AD  - Department of Microbiology, Federal Armed Forces of Germany, Bundeswehr 
      (Military) Academic Hospital, Berlin, Germany.
FAU - Muller, Martin
AU  - Muller M
AD  - Department of Microbiology, Federal Armed Forces of Germany, Bundeswehr 
      (Military) Academic Hospital, Berlin, Germany.
FAU - Stichling, Marcus
AU  - Stichling M
AD  - From the Trauma & Orthopedic Surgery, Septic & Reconstructive Surgery, Research 
      and Treatment Centre Septic Defect Wounds, Federal Armed Forces of Germany, 
      Bundeswehr (Military) Academic Hospital, Berlin, Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases, and Immunology, 
      Charite - University Medicine Berlin and MoKi Analytics GmbH, Berlin, Germany.
FAU - Willy, Christian
AU  - Willy C
AD  - From the Trauma & Orthopedic Surgery, Septic & Reconstructive Surgery, Research 
      and Treatment Centre Septic Defect Wounds, Federal Armed Forces of Germany, 
      Bundeswehr (Military) Academic Hospital, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20230522
PL  - United States
TA  - Plast Reconstr Surg Glob Open
JT  - Plastic and reconstructive surgery. Global open
JID - 101622231
PMC - PMC10287136
COIS- The authors have no financial interest to declare in relation to the content of 
      this article. This study was funded by the German Armed Forces as a special 
      research project (SoFo 08K4-S-121415) of the Department of Defence Medical 
      Research and Development. The funders had no role in study design, data 
      collection and analysis, decision to publish, or preparation of the article.
EDAT- 2023/06/26 19:07
MHDA- 2023/06/26 19:08
PMCR- 2023/05/22
CRDT- 2023/06/26 12:34
PHST- 2022/08/21 00:00 [received]
PHST- 2023/03/23 00:00 [accepted]
PHST- 2023/06/26 19:08 [medline]
PHST- 2023/06/26 19:07 [pubmed]
PHST- 2023/06/26 12:34 [entrez]
PHST- 2023/05/22 00:00 [pmc-release]
AID - 10.1097/GOX.0000000000004994 [doi]
PST - epublish
SO  - Plast Reconstr Surg Glob Open. 2023 May 22;11(5):e4994. doi: 
      10.1097/GOX.0000000000004994. eCollection 2023 May.