PMID- 37365039
OWN - NLM
STAT- MEDLINE
DCOM- 20240108
LR  - 20240108
IS  - 1440-1592 (Electronic)
IS  - 1323-8930 (Linking)
VI  - 73
IP  - 1
DP  - 2024 Jan
TI  - Contribution of monocyte and macrophage extracellular traps to neutrophilic 
      airway inflammation in severe asthma.
PG  - 81-93
LID - S1323-8930(23)00068-0 [pii]
LID - 10.1016/j.alit.2023.06.004 [doi]
AB  - BACKGROUND: Increased blood/sputum neutrophil counts are related to poor clinical 
      outcomes of severe asthma (SA), where we hypothesized that classical monocytes 
      (CMs)/CM-derived macrophages (Mphi) are involved. We aimed to elucidate the 
      mechanisms of how CMs/Mphi induce the activation of neutrophils/innate lymphoid 
      cells (ILCs) in SA. METHODS: Serum levels of monocyte chemoattractant protein-1 
      (MCP-1) and soluble suppression of tumorigenicity 2 (sST2) were measured from 39 
      patients with SA and 98 those with nonsevere asthma (NSA). CMs/Mphi were isolated 
      from patients with SA (n = 19) and those with NSA (n = 18) and treated with 
      LPS/interferon-gamma. Monocyte/M1Mphi extracellular traps (MoETs/M1ETs) were 
      evaluated by western blotting, immunofluorescence, and PicoGreen assay. The 
      effects of MoETs/M1ETs on neutrophils, airway epithelial cells (AECs), ILC1, and 
      ILC3 were assessed in vitro and in vivo. RESULTS: The SA group had significantly 
      higher CM counts with increased migration as well as higher levels of serum 
      MCP-1/sST2 than the NSA group. Moreover, the SA group had significantly greater 
      production of MoETs/M1ETs (from CMs/M1Mphi) than the NSA group. The levels of 
      MoETs/M1ETs were positively correlated with blood neutrophils and serum levels of 
      MCP-1/sST2, but negatively correlated with FEV(1)%. In vitro/in vivo studies 
      demonstrated that MoETs/M1ETs could activate AECs, neutrophils, ILC1, and ILC3 by 
      increased migration as well as proinflammatory cytokine production. CONCLUSIONS: 
      CM/Mphi-derived MoETs/M1ETs could contribute to asthma severity by enhancing 
      neutrophilic airway inflammation in SA, where modulating CMs/Mphi may be a 
      potential therapeutic option.
CI  - Copyright (c) 2023 Japanese Society of Allergology. Published by Elsevier B.V. All 
      rights reserved.
FAU - Quoc, Quang Luu
AU  - Quoc QL
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea; Department of Biomedical Sciences, Ajou University 
      School of Medicine, Suwon, South Korea.
FAU - Cao, Thi Bich Tra
AU  - Cao TBT
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea; Department of Biomedical Sciences, Ajou University 
      School of Medicine, Suwon, South Korea.
FAU - Moon, Ji-Young
AU  - Moon JY
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea.
FAU - Jang, Jae-Hyuk
AU  - Jang JH
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea.
FAU - Shin, Yoo Seob
AU  - Shin YS
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea.
FAU - Choi, Youngwoo
AU  - Choi Y
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea.
FAU - Ryu, Min Sook
AU  - Ryu MS
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea.
FAU - Park, Hae-Sim
AU  - Park HS
AD  - Department of Allergy and Clinical Immunology, Ajou University School of 
      Medicine, Suwon, South Korea; Department of Biomedical Sciences, Ajou University 
      School of Medicine, Suwon, South Korea. Electronic address: hspark@ajou.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20230624
PL  - England
TA  - Allergol Int
JT  - Allergology international : official journal of the Japanese Society of 
      Allergology
JID - 9616296
SB  - IM
MH  - Humans
MH  - *Extracellular Traps
MH  - Monocytes
MH  - Immunity, Innate
MH  - Lymphocytes
MH  - Neutrophils
MH  - *Asthma
MH  - Inflammation
MH  - Macrophages
OTO - NOTNLM
OT  - Asthma
OT  - Macrophages
OT  - Monocytes
OT  - Neutrophils
OT  - Severe asthma
EDAT- 2023/06/27 01:06
MHDA- 2024/01/08 06:42
CRDT- 2023/06/26 21:52
PHST- 2022/11/17 00:00 [received]
PHST- 2023/04/29 00:00 [revised]
PHST- 2023/05/16 00:00 [accepted]
PHST- 2024/01/08 06:42 [medline]
PHST- 2023/06/27 01:06 [pubmed]
PHST- 2023/06/26 21:52 [entrez]
AID - S1323-8930(23)00068-0 [pii]
AID - 10.1016/j.alit.2023.06.004 [doi]
PST - ppublish
SO  - Allergol Int. 2024 Jan;73(1):81-93. doi: 10.1016/j.alit.2023.06.004. Epub 2023 
      Jun 24.