PMID- 37366284
OWN - NLM
STAT- MEDLINE
DCOM- 20230731
LR  - 20230731
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 120
IP  - 8
DP  - 2023 Aug
TI  - Controlled release of adeno-associated virus from alginate hydrogel microbeads 
      with enhanced sensitivity to ultrasound.
PG  - 2371-2377
LID - 10.1002/bit.28482 [doi]
AB  - Adeno-associated virus (AAV)-based gene therapy holds promise as a fundamental 
      treatment for genetic disorders. For clinical applications, it is necessary to 
      control AAV release timing to avoid an immune response to AAV. Here we propose an 
      ultrasound (US)-triggered on-demand AAV release system using alginate hydrogel 
      microbeads (AHMs) with a release enhancer. By using a centrifuge-based 
      microdroplet shooting device, the AHMs encapsulating AAV with tungsten 
      microparticles (W-MPs) are fabricated. Since W-MPs work as release enhancers, the 
      AHMs have high sensitivity to the US with localized variation in acoustic 
      impedance for improving the release of AAV. Furthermore, AHMs were coated with 
      poly-l-lysine (PLL) to adjust the release of AAV. By applying US to the AAV 
      encapsulating AHMs with W-MPs, the AAV was released on demand, and gene 
      transfection to cells by AAV was confirmed without loss of AAV activity. This 
      proposed US-triggered AAV release system expands methodological possibilities in 
      gene therapy.
CI  - (c) 2023 The Authors. Biotechnology and Bioengineering published by Wiley 
      Periodicals LLC.
FAU - Takatsuka, Shuhei
AU  - Takatsuka S
AD  - School of Integrated Design Engineering, Graduate School of Science and 
      Technology, Keio University, Yokohama, Japan.
FAU - Kubota, Takeshi
AU  - Kubota T
AD  - School of Integrated Design Engineering, Graduate School of Science and 
      Technology, Keio University, Yokohama, Japan.
FAU - Kurashina, Yuta
AU  - Kurashina Y
AD  - Department of Mechanical Engineering, Faculty of Science and Technology, Keio 
      University, Yokohama, Japan.
AD  - Division of Advanced Mechanical Systems Engineering, Institute of Engineering, 
      Tokyo University of Agriculture and Technology, Tokyo, Japan.
FAU - Kurihara, Sho
AU  - Kurihara S
AD  - Department of Otorhinolaryngology, The Jikei University School of Medicine, 
      Tokyo, Japan.
AD  - Division of Regenerative Medicine, The Jikei University School of Medicine, 
      Tokyo, Japan.
FAU - Hirabayashi, Motoki
AU  - Hirabayashi M
AD  - Department of Otorhinolaryngology, The Jikei University School of Medicine, 
      Tokyo, Japan.
AD  - Division of Regenerative Medicine, The Jikei University School of Medicine, 
      Tokyo, Japan.
FAU - Fujioka, Masato
AU  - Fujioka M
AD  - Department of Molecular Genetics, Kitasato University School of Medicine, 
      Sagamihara, Kanagawa, Japan.
AD  - Clinical and Translational Research Center, Keio University Hospital, Tokyo, 
      Japan.
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Keio University School 
      of Medicine, Tokyo, Japan.
FAU - Okano, Hirotaka James
AU  - Okano HJ
AD  - Division of Regenerative Medicine, The Jikei University School of Medicine, 
      Tokyo, Japan.
FAU - Onoe, Hiroaki
AU  - Onoe H
AUID- ORCID: 0000-0003-0048-1580
AD  - School of Integrated Design Engineering, Graduate School of Science and 
      Technology, Keio University, Yokohama, Japan.
AD  - Department of Mechanical Engineering, Faculty of Science and Technology, Keio 
      University, Yokohama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230627
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Hydrogels)
RN  - 0 (Alginates)
RN  - 0 (Delayed-Action Preparations)
SB  - IM
MH  - *Hydrogels
MH  - *Dependovirus/genetics
MH  - Alginates
MH  - Microspheres
MH  - Delayed-Action Preparations
MH  - Genetic Vectors
OTO - NOTNLM
OT  - AAV
OT  - drug release
OT  - gene therapy
OT  - hydrogel microbeads
OT  - ultrasound
EDAT- 2023/06/27 13:10
MHDA- 2023/07/31 06:42
CRDT- 2023/06/27 07:03
PHST- 2023/06/10 00:00 [revised]
PHST- 2023/01/07 00:00 [received]
PHST- 2023/06/14 00:00 [accepted]
PHST- 2023/07/31 06:42 [medline]
PHST- 2023/06/27 13:10 [pubmed]
PHST- 2023/06/27 07:03 [entrez]
AID - 10.1002/bit.28482 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2023 Aug;120(8):2371-2377. doi: 10.1002/bit.28482. Epub 2023 
      Jun 27.