PMID- 37368598 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230701 IS - 2305-6304 (Electronic) IS - 2305-6304 (Linking) VI - 11 IP - 6 DP - 2023 May 31 TI - Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples. LID - 10.3390/toxics11060498 [doi] LID - 498 AB - Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient options. In this study, we have applied and validated a method for detecting several hundred toxins in serum samples. This technique involved a single-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by analysis using gas and liquid chromatography coupled with mass spectrometry. With this methodology, we could detect and quantify up to 353 compounds, including persistent organic pollutants (POPs), pesticides, pharmaceuticals, and rodenticides, using just 250 microL of serum. Among them, 92% could be measured at concentrations below 1.25 ng/mL, making it ideal for biomonitoring. We applied this method to samples collected from camels (n = 40) and humans (n = 25). We detected naproxen, ketoprofen, paracetamol, levamisole, and some POPs in these samples. This study validated the ability to simultaneously detect a broad range of compounds in small volumes of serum. FAU - Rial-Berriel, Cristian AU - Rial-Berriel C AUID- ORCID: 0000-0003-2773-8075 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. FAU - Ramos-Luzardo, Alvaro AU - Ramos-Luzardo A AUID- ORCID: 0009-0002-9435-2228 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. FAU - Acosta-Dacal, Andrea AU - Acosta-Dacal A AUID- ORCID: 0000-0002-1272-0545 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. FAU - Macias-Montes, Ana AU - Macias-Montes A AUID- ORCID: 0000-0001-8554-5728 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. FAU - Fernandez-Valeron, Pilar AU - Fernandez-Valeron P AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. FAU - Henriquez-Hernandez, Luis Alberto AU - Henriquez-Hernandez LA AUID- ORCID: 0000-0003-3237-0316 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. AD - Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Zumbado, Manuel AU - Zumbado M AUID- ORCID: 0000-0002-1534-7758 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. AD - Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Boada, Luis D AU - Boada LD AUID- ORCID: 0000-0002-0195-4565 AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. AD - Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Luzardo, Octavio P AU - Luzardo OP AD - Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s/n, 35016 Las Palmas de Gran Canaria, Spain. AD - Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain. LA - eng GR - (APCR2022010002)/Catalina Ruiz research staff training aid program of the Regional Ministry of Economy, Knowledge, and Employment of the Canary Islands Government and the European Social Fund granted to the University of Las Palmas de Gran Canaria via a post-doctoral gran/ GR - APCR2022010003/Catalina Ruiz research staff training aid program of the Regional Ministry of Economy, Knowledge, and Employment of the Canary Islands Government and the European Social Fund granted to the University of Las Palmas de Gran Canaria via a post-doctoral gran/ GR - (ULPGC-2021-2022)./a pre-doctoral grant from the University of Las Palmas de Gran Canaria/ PT - Journal Article DEP - 20230531 PL - Switzerland TA - Toxics JT - Toxics JID - 101639637 PMC - PMC10305645 OTO - NOTNLM OT - POPs OT - biomonitoring OT - pesticides OT - pharmaceuticals OT - serum COIS- The authors declare no conflict of interest. EDAT- 2023/06/27 19:13 MHDA- 2023/06/27 19:14 PMCR- 2023/05/31 CRDT- 2023/06/27 12:23 PHST- 2023/04/19 00:00 [received] PHST- 2023/05/17 00:00 [revised] PHST- 2023/05/29 00:00 [accepted] PHST- 2023/06/27 19:14 [medline] PHST- 2023/06/27 19:13 [pubmed] PHST- 2023/06/27 12:23 [entrez] PHST- 2023/05/31 00:00 [pmc-release] AID - toxics11060498 [pii] AID - toxics-11-00498 [pii] AID - 10.3390/toxics11060498 [doi] PST - epublish SO - Toxics. 2023 May 31;11(6):498. doi: 10.3390/toxics11060498.