PMID- 37371838
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230701
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 11
IP  - 6
DP  - 2023 Jun 17
TI  - Mannose-Binding Lectin Reduces Oxidized Low-Density Lipoprotein Induced Vascular 
      Endothelial Cells Injury by Inhibiting LOX1-ox-LDL Binding and Modulating 
      Autophagy.
LID - 10.3390/biomedicines11061743 [doi]
LID - 1743
AB  - Objective: To investigate the role of mannose-binding lectin (MBL) in modulating 
      autophagy and protecting endothelial cells (ECs) from oxidized low-density 
      lipoprotein (ox-LDL)-induced injury. Methods: Serum MBL concentration and carotid 
      intima-media thickness (cIMT) were measured in 94 obese and 105 healthy children. 
      ECs were transfected with MBL over-expression plasmid, LOX1 was knocked-down to 
      explore the protective role of MBL in ox-LDL induced ECs injury. Dendritic cells 
      (DCs) were co-cultured with ECs, and inflammatory factors, DC maturation, and 
      autophagy was assessed. WT and ApoE(-/-) mice were fed with a high fat diet (HFD) 
      with or without MBL-adenovirus injection for 16 weeks and aortic vascular 
      endothelial tissue was isolated, then atherosclerotic plaque, cell injury and 
      autophagy were analyzed. Results: Serum MBL concentration in obese children was 
      lower than healthy controls and was negatively correlated with cIMT. The uptake 
      of ox-LDL was decreased in LOX1 knock-down ECs. MBL over-expression in vitro 
      inhibited LOX1-ox-LDL binding. Both LOX1 knock-down and MBL over-expression can 
      ameliorate EC autophagy and cell injury. MBL over-expression in vivo alleviated 
      atherosclerotic plaque formation, influenced DC maturation and down-regulated 
      IL-6, IL-12, and TNF-a levels. Conclusions: MBL exerts a protective role in 
      ox-LDL-induced EC injury by modulating DC maturation and EC autophagy via 
      inhibiting LOX1-ox-LDL binding.
FAU - Zhou, Xuelian
AU  - Zhou X
AUID- ORCID: 0000-0002-0240-0652
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Chen, Xuefeng
AU  - Chen X
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Yuan, Jinna
AU  - Yuan J
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Lin, Hu
AU  - Lin H
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Zhu, Mingqiang
AU  - Zhu M
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Xu, Xiaoqin
AU  - Xu X
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Dong, Guanping
AU  - Dong G
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Fu, Junfen
AU  - Fu J
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Endocrinology, Children's Hospital, Zhejiang University School of 
      Medicine, National Clinical Research Center for Child Health, 3333 Binsheng Road, 
      Hangzhou 310052, China.
LA  - eng
GR  - 81471056/National Natural Science Foundation of China/
GR  - 2022KY870/the Clinical research application project of Zhejiang Province Health 
      science and Technology Plan/
GR  - 2021YFC2701901/the National Key Research and Development Program of China/
GR  - LSZ19H070001/Zhejiang Province Natural Sciences Foundation Zhejiang Society for 
      Mathematical Medicine/
GR  - LGF21H070004/Zhejiang Provincial Key Science and Technology Project/
GR  - 2020XZZX002-22/the Fundamental Research Funds for the Central Universities/
PT  - Journal Article
DEP - 20230617
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC10296346
OTO - NOTNLM
OT  - LOX1
OT  - atherosclerosis
OT  - autophagy
OT  - endothelial cells injury
OT  - mannose-binding lectin
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/28 06:42
MHDA- 2023/06/28 06:43
PMCR- 2023/06/17
CRDT- 2023/06/28 01:09
PHST- 2023/04/23 00:00 [received]
PHST- 2023/06/05 00:00 [revised]
PHST- 2023/06/14 00:00 [accepted]
PHST- 2023/06/28 06:43 [medline]
PHST- 2023/06/28 06:42 [pubmed]
PHST- 2023/06/28 01:09 [entrez]
PHST- 2023/06/17 00:00 [pmc-release]
AID - biomedicines11061743 [pii]
AID - biomedicines-11-01743 [pii]
AID - 10.3390/biomedicines11061743 [doi]
PST - epublish
SO  - Biomedicines. 2023 Jun 17;11(6):1743. doi: 10.3390/biomedicines11061743.