PMID- 37371950
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230701
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 12
IP  - 6
DP  - 2023 Jun 5
TI  - Liposome-Encapsulated Berberine Alleviates Liver Injury in Type 2 Diabetes via 
      Promoting AMPK/mTOR-Mediated Autophagy and Reducing ER Stress: Morphometric and 
      Immunohistochemical Scoring.
LID - 10.3390/antiox12061220 [doi]
LID - 1220
AB  - In the advanced stages of type 2 diabetes mellitus (T2DM), diabetic liver damage 
      is a common complication that can devastate a patient's quality of life. The 
      present study investigated the ability of liposomal berberine (Lip-BBR) to aid in 
      ameliorating hepatic damage and steatosis, insulin homeostasis, and regulating 
      lipid metabolism in type 2 diabetes (T2DM) and the possible pathways by which it 
      does so. Liver tissue microarchitectures and immunohistochemical staining were 
      applied during the study. The rats were divided into a control non-diabetic group 
      and four diabetic groups, which are the T2DM, T2DM-Lip-BBR (10 mg/kg b.wt), 
      T2DM-Vildagliptin (Vild) (10 mg/kg b.wt), and T2DM-BBR-Vild (10 mg/kg b.wt + Vild 
      (5 mg/kg b.wt) groups. The findings demonstrated that Lip-BBR treatment could 
      restore liver tissue microarchitectures, reduce steatosis and liver function, and 
      regulate lipid metabolism. Moreover, Lip-BBR treatment promoted autophagy via the 
      activation of LC3-II and Bclin-1 proteins and activated the AMPK/mTOR pathway in 
      the liver tissue of T2DM rats. Lip-BBR also activated the GLP-1 expression, which 
      stimulated insulin biosynthesis. It decreased the endoplasmic reticulum stress by 
      limiting the CHOP, JNK expression, oxidative stress, and inflammation. 
      Collectively, Lip-BBR ameliorated diabetic liver injury in a T2DM rat model with 
      its promotion activity of AMPK/mTOR-mediated autophagy and limiting ER stress.
FAU - Khater, Safaa I
AU  - Khater SI
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig 44511, Egypt.
FAU - Almanaa, Taghreed N
AU  - Almanaa TN
AD  - Department of Botany and Microbiology, College of Science, King Saud University, 
      Riyadh 11451, Saudi Arabia.
FAU - Fattah, Doaa M Abdel
AU  - Fattah DMA
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig 44511, Egypt.
FAU - Khamis, Tarek
AU  - Khamis T
AD  - Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig 44519, Egypt.
AD  - Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig 44519, Egypt.
FAU - Seif, Mona M
AU  - Seif MM
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig 44511, Egypt.
FAU - Dahran, Naief
AU  - Dahran N
AUID- ORCID: 0000-0003-3560-8166
AD  - Department of Anatomy, Faculty of Medicine, University of Jeddah, Jeddah 80203, 
      Saudi Arabia.
FAU - Alqahtani, Leena S
AU  - Alqahtani LS
AD  - Department of Biochemistry, College of Science, University of Jeddah, Jeddah 
      80203, Saudi Arabia.
FAU - Metwally, Mohamed M M
AU  - Metwally MMM
AD  - Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig 44511, Egypt.
FAU - Mostafa, Mahmoud
AU  - Mostafa M
AUID- ORCID: 0000-0001-6425-9247
AD  - Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia 61519, 
      Egypt.
FAU - Albedair, Raghad A
AU  - Albedair RA
AD  - Department of Botany and Microbiology, College of Science, King Saud University, 
      Riyadh 11451, Saudi Arabia.
FAU - Helal, Azza I
AU  - Helal AI
AD  - Department of Histology and Cell Biology, Faculty of Medicine, Kafrelsheikh 
      University, Kafrelsheikh 33516, Egypt.
FAU - Alosaimi, Manal
AU  - Alosaimi M
AUID- ORCID: 0000-0001-9951-0294
AD  - Department of Basic Medical Sciences, College of Medicine, Princess Nourah Bint 
      Abdulrahman University, Riyadh 11671, Saudi Arabia.
FAU - Mohamed, Amany Abdel-Rahman
AU  - Mohamed AA
AUID- ORCID: 0000-0003-0061-2675
AD  - Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, 
      Zagazig University, Zagazig 44511, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20230605
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC10295074
OTO - NOTNLM
OT  - AMPK/mTOR
OT  - Bclin-1
OT  - ER stress
OT  - autophagy
OT  - liposomal berberine
OT  - type 2 diabetes
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/28 06:42
MHDA- 2023/06/28 06:43
PMCR- 2023/06/05
CRDT- 2023/06/28 01:10
PHST- 2023/05/04 00:00 [received]
PHST- 2023/05/24 00:00 [revised]
PHST- 2023/05/30 00:00 [accepted]
PHST- 2023/06/28 06:43 [medline]
PHST- 2023/06/28 06:42 [pubmed]
PHST- 2023/06/28 01:10 [entrez]
PHST- 2023/06/05 00:00 [pmc-release]
AID - antiox12061220 [pii]
AID - antioxidants-12-01220 [pii]
AID - 10.3390/antiox12061220 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2023 Jun 5;12(6):1220. doi: 10.3390/antiox12061220.