PMID- 37372998
OWN - NLM
STAT- MEDLINE
DCOM- 20230629
LR  - 20230701
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 12
DP  - 2023 Jun 7
TI  - BCL11A Expression in Non-Small Cell Lung Cancers.
LID - 10.3390/ijms24129848 [doi]
LID - 9848
AB  - B-cell leukemia/lymphoma 11A (BCL11A) may be one of the potential biomarkers of 
      non-small cell lung cancer (NSCLC). However, its role in the development of this 
      cancer has not yet been precisely established. The aim of this study was to 
      investigate the expression of BCL11A at the mRNA and protein levels in NSCLC 
      cases and non-malignant lung tissue (NMLT) and to determine the relationship 
      between BCL11A expression and the clinicopathological factors and Ki-67, Slug, 
      Snail and Twist. The localization and the level of BCL11A protein were examined 
      using immunohistochemistry (IHC) on 259 cases of NSCLC, and 116 NMLT samples were 
      prepared as tissue microarrays and using immunofluorescence (IF) in the following 
      cell lines: NCI-H1703, A549 and IMR-90. The mRNA expression of BCL11A was 
      determined using real-time PCR in 33 NSCLC cases, 10 NMLT samples and the cell 
      lines. BCL11A protein expression was significantly higher in NSCLC cases compared 
      to NMLT. Nuclear expression was found in lung squamous cell carcinoma (SCC) 
      cells, while cytoplasmic expression was demonstrated in adenocarcinoma (AC) 
      cells. Nuclear expression of BCL11A decreased with increasing malignancy grade 
      and correlated positively with Ki-67 and Slug and Twist expression. The opposite 
      relationships were found for the cytoplasmic expression of BCL11A. Nuclear 
      expression of BCL11A in NSCLC cells may affect tumor cell proliferation and 
      change their phenotype, thus promoting tumor progression.
FAU - Katnik, Ewa
AU  - Katnik E
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Gomulkiewicz, Agnieszka
AU  - Gomulkiewicz A
AUID- ORCID: 0000-0003-2256-1055
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Piotrowska, Aleksandra
AU  - Piotrowska A
AUID- ORCID: 0000-0003-4093-7386
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Grzegrzolka, Jedrzej
AU  - Grzegrzolka J
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Kmiecik, Alicja
AU  - Kmiecik A
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Ratajczak-Wielgomas, Katarzyna
AU  - Ratajczak-Wielgomas K
AUID- ORCID: 0000-0001-6175-2204
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Urbaniak, Anna
AU  - Urbaniak A
AUID- ORCID: 0000-0002-2803-0952
AD  - Department of Biochemistry and Molecular Biology, Wroclaw University of 
      Environmental and Life Sciences, 50-375 Wroclaw, Poland.
FAU - Glatzel-Plucinska, Natalia
AU  - Glatzel-Plucinska N
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Blasiak, Piotr
AU  - Blasiak P
AUID- ORCID: 0000-0002-5694-0638
AD  - Department and Clinic of Thoracic Surgery, Wroclaw Medical University, 53-439 
      Wroclaw, Poland.
AD  - Lower Silesian Center of Oncology, Pulmonology and Hematology, 53-439 Wroclaw, 
      Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
LA  - eng
GR  - STM.A110.17.022/Wroclaw Medical University/
PT  - Journal Article
DEP - 20230607
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (BCL11A protein, human)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Humans
MH  - *Carcinoma, Non-Small-Cell Lung/pathology
MH  - *Lung Neoplasms/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Transcription Factors/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Repressor Proteins/genetics/metabolism
PMC - PMC10297982
OTO - NOTNLM
OT  - BCL11A
OT  - Ki-67
OT  - non-small cell lung cancer
OT  - slug
OT  - snail
OT  - twist
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/28 06:42
MHDA- 2023/06/29 06:43
PMCR- 2023/06/07
CRDT- 2023/06/28 01:16
PHST- 2023/04/30 00:00 [received]
PHST- 2023/05/28 00:00 [revised]
PHST- 2023/06/04 00:00 [accepted]
PHST- 2023/06/29 06:43 [medline]
PHST- 2023/06/28 06:42 [pubmed]
PHST- 2023/06/28 01:16 [entrez]
PHST- 2023/06/07 00:00 [pmc-release]
AID - ijms24129848 [pii]
AID - ijms-24-09848 [pii]
AID - 10.3390/ijms24129848 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jun 7;24(12):9848. doi: 10.3390/ijms24129848.