PMID- 37375371
OWN - NLM
STAT- MEDLINE
DCOM- 20230629
LR  - 20230701
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 12
DP  - 2023 Jun 16
TI  - Characterization of Polyphenols from Chenopodium botrys after Fractionation with 
      Different Solvents and Study of Their In Vitro Biological Activity.
LID - 10.3390/molecules28124816 [doi]
LID - 4816
AB  - In the present work, we have investigated the polyphenolic composition of 
      Chenopodium botrys from Bulgaria. The polyphenols were fractionated with solvents 
      of varying polarity (n-hexane, chloroform, ethyl acetate, and n-butanol). The 
      fractions were analyzed by HPLC-PDA and UHPLC-MS. The ethyl acetate fraction 
      contained mono- and di-glycosides of quercetin, di-glycosides of kaempferol, and 
      isorhamnetin and monoglycosides of hispidulin and jaceosidine. We found quercetin 
      triglycosides in the butanol fraction. The ethyl acetate and butanol fractions 
      contained 168.82 mg/g Extr and 67.21 mg/g Extr of quercetin glycosides, 
      respectively. The main components of the polyphenolic complex in C. botrys were 
      6-methoxyflavones (355.47 mg/g Extr), which were found in the chloroform 
      fraction. The flavonoids pectolinarigenin, demethylnobiletin, and isosinensetin, 
      and the glycosides of quercetin (triglycosides, acylglycosides), kaempferol, 
      isorhamnetin, hispidiulin, and jaceosidine, were discovered and reported in 
      Chenopodium botrys for the first time. We used in vitro methods to assess the 
      biological activity against oxidative stress (hydrogen peroxide scavenging 
      activity (HPSA) and hydroxyl radical scavenging activity (HRSA)), nitrosative 
      stress (nitric oxide scavenging activity (NOSA)), anti-inflammatory activity (IAD 
      inhibition), and anti-tryptic activity (ATA). Quercetin mono- and di-glycosides 
      exhibited greater HPSA and HRSA (IC(50) = 39.18, 105.03 microg/mL), while 
      6-methoxyflavones had a greater NOSA (IC(50) = 146.59 microg/mL). The same components 
      showed the highest ATA (IC(50) ranging from 116.23 to 202.44 microg/mL).
FAU - Bojilov, Dimitar
AU  - Bojilov D
AUID- ORCID: 0000-0001-6569-8348
AD  - Department of Organic Chemistry, Faculty of Chemistry, University of Plovdiv, 
      4000 Plovdiv, Bulgaria.
FAU - Manolov, Stanimir
AU  - Manolov S
AUID- ORCID: 0000-0002-0314-6553
AD  - Department of Organic Chemistry, Faculty of Chemistry, University of Plovdiv, 
      4000 Plovdiv, Bulgaria.
FAU - Nacheva, Angelika
AU  - Nacheva A
AD  - Department of Organic Chemistry, Faculty of Chemistry, University of Plovdiv, 
      4000 Plovdiv, Bulgaria.
FAU - Dagnon, Soleya
AU  - Dagnon S
AUID- ORCID: 0000-0001-6570-4947
AD  - Department of Organic Chemistry, Faculty of Chemistry, University of Plovdiv, 
      4000 Plovdiv, Bulgaria.
FAU - Ivanov, Iliyan
AU  - Ivanov I
AUID- ORCID: 0000-0002-4662-3735
AD  - Department of Organic Chemistry, Faculty of Chemistry, University of Plovdiv, 
      4000 Plovdiv, Bulgaria.
LA  - eng
GR  - capital EM, Cyrilliccapital U, Cyrillic21-capital HA, Cyrilliccapital EF, Cyrillic-014 and capital EF, Cyrilliccapital PE, Cyrillic23-capital HA, Cyrilliccapital EF, Cyrillic-005/Plovdiv University/
PT  - Journal Article
DEP - 20230616
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Polyphenols)
RN  - 76845O8NMZ (ethyl acetate)
RN  - 0 (Solvents)
RN  - 0 (Antioxidants)
RN  - 9IKM0I5T1E (Quercetin)
RN  - 0 (Kaempferols)
RN  - 7V31YC746X (Chloroform)
RN  - 0 (Plant Extracts)
RN  - 0 (Flavonoids)
RN  - 0 (Glycosides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 0 (Butanols)
SB  - IM
MH  - *Polyphenols/pharmacology
MH  - Solvents
MH  - Antioxidants/pharmacology
MH  - Quercetin
MH  - Kaempferols/pharmacology
MH  - Chloroform
MH  - Plant Extracts/pharmacology
MH  - Flavonoids/pharmacology
MH  - Glycosides/pharmacology
MH  - Nitric Oxide
MH  - *Chenopodium
MH  - Butanols
PMC - PMC10300873
OTO - NOTNLM
OT  - 6-methoxy and polymethoxylated flavones
OT  - ATA
OT  - Chenopodium botrys
OT  - HPSA
OT  - HRSA
OT  - IAD
OT  - NOSA
OT  - flavonoid-glycosides
OT  - in vitro biological activity
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/28 06:42
MHDA- 2023/06/29 06:43
PMCR- 2023/06/16
CRDT- 2023/06/28 01:32
PHST- 2023/05/19 00:00 [received]
PHST- 2023/06/14 00:00 [revised]
PHST- 2023/06/14 00:00 [accepted]
PHST- 2023/06/29 06:43 [medline]
PHST- 2023/06/28 06:42 [pubmed]
PHST- 2023/06/28 01:32 [entrez]
PHST- 2023/06/16 00:00 [pmc-release]
AID - molecules28124816 [pii]
AID - molecules-28-04816 [pii]
AID - 10.3390/molecules28124816 [doi]
PST - epublish
SO  - Molecules. 2023 Jun 16;28(12):4816. doi: 10.3390/molecules28124816.