PMID- 37376102
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230701
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 15
IP  - 6
DP  - 2023 Jun 4
TI  - Mesenchymal Stem Cell Membrane-Coated TPCS(2a)-Loaded Nanoparticles for Breast 
      Cancer Photodynamic Therapy.
LID - 10.3390/pharmaceutics15061654 [doi]
LID - 1654
AB  - Despite substantial improvements in breast cancer (BC) treatment there is still 
      an urgent need to find alternative treatment options to improve the outcomes for 
      patients with advanced-stage disease. Photodynamic therapy (PDT) is gaining a lot 
      of attention as a BC therapeutic option because of its selectivity and low 
      off-target effects. However, the hydrophobicity of photosensitizers (PSs) impairs 
      their solubility and limits the circulation in the bloodstream, thus representing 
      a major challenge. The use of polymeric nanoparticles (NPs) to encapsulate the PS 
      may represent a valuable strategy to overcome these issues. Herein, we developed 
      a novel biomimetic PDT nanoplatform (NPs) based on a polymeric core of 
      poly(lactic-co-glycolic)acid (PLGA) loaded with the PS meso-tetraphenylchlorin 
      disulfonate (TPCS(2a)). TPCS(2a)@NPs of 98.89 +/- 18.56 nm with an encapsulation 
      efficiency percentage (EE%) of 81.9 +/- 7.92% were obtained and coated with 
      mesenchymal stem cells-derived plasma membranes (mMSCs) (mMSC-TPCS(2a)@NPs, size 
      of 139.31 +/- 12.94 nm). The mMSC coating armed NPs with biomimetic features to 
      impart long circulation times and tumor-homing capabilities. In vitro, biomimetic 
      mMSC-TPCS(2a)@NPs showed a decrease in macrophage uptake of 54% to 70%, depending 
      on the conditions applied, as compared to uncoated TPCS(2a)@NPs. Both NP 
      formulations efficiently accumulated in MCF7 and MDA-MB-231 BC cells, while the 
      uptake was significantly lower in normal breast epithelial MCF10A cells with 
      respect to tumor cells. Moreover, encapsulation of TPCS(2a) in mMSC-TPCS(2a)@NPs 
      effectively prevents its aggregation, ensuring efficient singlet oxygen ((1)O(2)) 
      production after red light irradiation, which resulted in a considerable in vitro 
      anticancer effect in both BC cell monolayers (IC(50) < 0.15 microM) and 
      three-dimensional spheroids.
FAU - Avancini, Greta
AU  - Avancini G
AUID- ORCID: 0000-0001-8792-342X
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, 
      Italy.
FAU - Menilli, Luca
AU  - Menilli L
AUID- ORCID: 0000-0003-2774-6411
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, 
      Italy.
FAU - Visentin, Adele
AU  - Visentin A
AD  - Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 
      Via F. Marzolo 5, 35131 Padova, Italy.
FAU - Milani, Celeste
AU  - Milani C
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, 
      Italy.
FAU - Mastrotto, Francesca
AU  - Mastrotto F
AUID- ORCID: 0000-0002-2499-5490
AD  - Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 
      Via F. Marzolo 5, 35131 Padova, Italy.
FAU - Moret, Francesca
AU  - Moret F
AUID- ORCID: 0000-0002-1534-3714
AD  - Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, 
      Italy.
LA  - eng
PT  - Journal Article
DEP - 20230604
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC10302938
OTO - NOTNLM
OT  - biomimetic nanoparticles
OT  - breast cancer
OT  - photodynamic therapy
COIS- The authors declare no conflict of interest.
EDAT- 2023/06/28 06:42
MHDA- 2023/06/28 06:43
PMCR- 2023/06/04
CRDT- 2023/06/28 01:37
PHST- 2023/05/03 00:00 [received]
PHST- 2023/05/26 00:00 [revised]
PHST- 2023/05/31 00:00 [accepted]
PHST- 2023/06/28 06:43 [medline]
PHST- 2023/06/28 06:42 [pubmed]
PHST- 2023/06/28 01:37 [entrez]
PHST- 2023/06/04 00:00 [pmc-release]
AID - pharmaceutics15061654 [pii]
AID - pharmaceutics-15-01654 [pii]
AID - 10.3390/pharmaceutics15061654 [doi]
PST - epublish
SO  - Pharmaceutics. 2023 Jun 4;15(6):1654. doi: 10.3390/pharmaceutics15061654.