PMID- 37377307
OWN - NLM
STAT- MEDLINE
DCOM- 20230629
LR  - 20230629
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Linking)
VI  - 56
DP  - 2023
TI  - Brain glucose hypometabolism and hippocampal inflammation in Goto-Kakizaki rats.
PG  - e12742
LID - S0100-879X2023000100640 [pii]
LID - 10.1590/1414-431X2023e12742 [doi]
AB  - Brain glucose hypometabolism and neuroinflammation are early pathogenic 
      manifestations in neurological disorders. Neuroinflammation may also disrupt 
      leptin signaling, an adipokine that centrally regulates appetite and energy 
      balance by acting on the hypothalamus and exerting neuroprotection in the 
      hippocampus. The Goto-Kakizaki (GK) rat is a non-obese type 2 diabetes mellitus 
      (T2DM) animal model used to investigate diabetes-associated molecular mechanisms 
      without obesity jeopardizing effects. Wistar and GK rats received the maintenance 
      adult rodent diet. Also, an additional control group of Wistar rats received a 
      high-fat and high-sugar diet (HFHS) provided by free consumption of condensed 
      milk. All diets and water were provided ad libitum for eight weeks. Brain glucose 
      uptake was evaluated by 2-deoxy-2-[fluorine-18] fluoro-D-glucose under basal 
      (saline administration) or stimulated (CL316,243, a selective beta3-AR agonist) 
      conditions. The animals were fasted for 10-12 h, anesthetized, and euthanized. 
      The brain was quickly dissected, and the hippocampal area was sectioned and 
      stored at -80 degrees C in different tubes for protein and RNA analyses on the same 
      animal. GK rats exhibited attenuated brain glucose uptake compared to Wistar 
      animals and the HFHS group under basal conditions. Also, the hippocampus of GK 
      rats displayed upregulated leptin receptor, IL-1beta, and IL-6 gene expression and 
      IL-1beta and the subunit of the transcription factor NF-kappaB (p-p65) protein 
      expression. No significant alterations were detected in the hippocampus of HFHS 
      rats. Our data indicated that a genetic predisposition to T2DM has significant 
      brain deteriorating features, including brain glucose hypometabolism, 
      neuroinflammation, and leptin signaling disruption in the hippocampal area.
FAU - Borges, J C O
AU  - Borges JCO
AUID- ORCID: 0000-0002-0196-0156
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Oliveira, V A B
AU  - Oliveira VAB
AUID- ORCID: 0000-0002-9352-6735
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Serdan, T D A
AU  - Serdan TDA
AUID- ORCID: 0000-0002-1977-0529
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Silva, F L R
AU  - Silva FLR
AUID- ORCID: 0000-0002-8917-4618
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Santos, C S
AU  - Santos CS
AUID- ORCID: 0000-0002-5631-440X
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Pauferro, J R B
AU  - Pauferro JRB
AUID- ORCID: 0000-0001-5973-8008
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Ribas, A S F
AU  - Ribas ASF
AUID- ORCID: 0000-0002-2189-9735
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Manoel, R
AU  - Manoel R
AUID- ORCID: 0000-0002-2433-2873
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Pereira, A C G
AU  - Pereira ACG
AUID- ORCID: 0000-0002-4338-9138
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Correa, I S
AU  - Correa IS
AUID- ORCID: 0000-0001-9325-8067
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Pereira, J N B
AU  - Pereira JNB
AUID- ORCID: 0000-0003-2609-5450
AD  - Secao de Producao de Imunobiologicos, Bioindustrial Centro, Instituto Butantan, 
      Sao Paulo, SP, Brasil.
FAU - Bazotte, R B
AU  - Bazotte RB
AUID- ORCID: 0000-0001-8563-7913
AD  - Programa de Pos-Graduacao em Ciencias Farmaceuticas, Departamento de Farmacologia 
      e Terapeutica, Universidade Estadual de Maringa, Maringa, PR, Brasil.
FAU - Levada-Pires, A C
AU  - Levada-Pires AC
AUID- ORCID: 0000-0002-5320-5953
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Pithon-Curi, T C
AU  - Pithon-Curi TC
AUID- ORCID: 0000-0002-9714-9105
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Gorjao, R
AU  - Gorjao R
AUID- ORCID: 0000-0002-9655-1477
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Curi, R
AU  - Curi R
AUID- ORCID: 0000-0001-5095-9154
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
AD  - Secao de Producao de Imunobiologicos, Bioindustrial Centro, Instituto Butantan, 
      Sao Paulo, SP, Brasil.
FAU - Hirabara, S M
AU  - Hirabara SM
AUID- ORCID: 0000-0002-7392-0444
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
FAU - Masi, L N
AU  - Masi LN
AUID- ORCID: 0000-0001-9472-7304
AD  - Programa Interdisciplinar de Ciencias da Saude, Universidade Cruzeiro do Sul, Sao 
      Paulo, SP, Brasil.
AD  - Departamento de Ciencias Fisiologicas, Centro de Ciencias Biologicas, 
      Universidade Federal de Santa Catarina, Florianopolis, SC, Brasil.
LA  - eng
PT  - Journal Article
DEP - 20230626
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (Leptin)
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Glucose/metabolism
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Rats, Wistar
MH  - Leptin
MH  - Blood Glucose/metabolism
MH  - Neuroinflammatory Diseases
MH  - Brain/metabolism
MH  - Obesity
MH  - Hippocampus/metabolism
MH  - Inflammation
MH  - Insulin
EDAT- 2023/06/28 13:09
MHDA- 2023/06/29 06:42
CRDT- 2023/06/28 08:16
PHST- 2023/04/10 00:00 [received]
PHST- 2023/05/05 00:00 [accepted]
PHST- 2023/06/29 06:42 [medline]
PHST- 2023/06/28 13:09 [pubmed]
PHST- 2023/06/28 08:16 [entrez]
AID - S0100-879X2023000100640 [pii]
AID - 10.1590/1414-431X2023e12742 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2023 Jun 26;56:e12742. doi: 10.1590/1414-431X2023e12742. 
      eCollection 2023.