PMID- 37382653
OWN - NLM
STAT- MEDLINE
DCOM- 20230823
LR  - 20230915
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 415
IP  - 22
DP  - 2023 Sep
TI  - A sample-preparation-free, point-of-care testing system for in situ detection of 
      bovine mastitis.
PG  - 5499-5509
LID - 10.1007/s00216-023-04823-3 [doi]
AB  - We present a highly integrated point-of-care testing (POCT) device capable of 
      immediately and accurately screening bovine mastitis infection based on somatic 
      cell counting (SCC). The system primarily consists of a homemade cell-counting 
      chamber and a miniature fluorescent microscope. The cell-counting chamber is 
      pre-embedded with acridine orange (AO) in advance, which is simple and practical. 
      And then SCC is directly identified by microscopic imaging analysis to evaluate 
      the bovine mastitis infection. Only 4 muL of raw bovine milk is required for a 
      simple sample testing and accurate SCC. The entire assay process from sampling to 
      result in presentation is completed quickly within 6 min, enabling instant 
      "sample-in and answer-out." Under laboratory conditions, we mixed bovine 
      leukocyte suspension with whole milk and achieved a detection limit as low as 
      2.12 x 10(4) cells/mL on the system, which is capable of screening various types 
      of clinical standards of bovine milk. The fitting degrees of the proposed POCT 
      system with manual fluorescence microscopy were generally consistent 
      (R(2) > 0.99). As a proof of concept, four fresh milk samples were used in the 
      test. The average accuracy of somatic cell counts was 98.0%, which was able to 
      successfully differentiate diseased cows from healthy ones. The POCT system is 
      user-friendly and low-cost, making it a potential tool for on-site diagnosis of 
      bovine mastitis in resource-limited areas.
CI  - (c) 2023. Springer-Verlag GmbH Germany, part of Springer Nature.
FAU - He, Lei
AU  - He L
AD  - School of Electrical and Information Engineering, Zhengzhou University, 
      Zhengzhou, 450001, Henan, China.
FAU - Chen, Bing
AU  - Chen B
AD  - Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052, Henan, China.
FAU - Hu, Yu
AU  - Hu Y
AD  - School of Electrical and Information Engineering, Zhengzhou University, 
      Zhengzhou, 450001, Henan, China.
FAU - Hu, Boheng
AU  - Hu B
AD  - School of Electrical and Information Engineering, Zhengzhou University, 
      Zhengzhou, 450001, Henan, China.
FAU - Li, Ya
AU  - Li Y
AD  - Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052, Henan, China. liya0305@126.com.
FAU - Yang, Xiaonan
AU  - Yang X
AD  - School of Electrical and Information Engineering, Zhengzhou University, 
      Zhengzhou, 450001, Henan, China. iexnyang@zzu.edu.cn.
AD  - National Center for International Joint Research of Electronic Materials and 
      Systems, International Joint-Laboratory of Electronic Materials and Systems of 
      Henan Province, School of Information Engineering, Zhengzhou University, 
      Zhengzhou, 450001, Henan, China. iexnyang@zzu.edu.cn.
LA  - eng
GR  - XDB44000000/Strategic Priority Research Program of the Chinese Academy of 
      Sciences/
GR  - 232102311187/The Science and Technology Research Program of Henan Province/
PT  - Journal Article
DEP - 20230629
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
SB  - IM
MH  - Animals
MH  - Female
MH  - Cattle
MH  - *Mastitis, Bovine/diagnosis
MH  - Milk/metabolism
MH  - Point-of-Care Testing
MH  - Microscopy, Fluorescence
MH  - Cell Count/methods
OTO - NOTNLM
OT  - Bovine mastitis
OT  - Fluorescent staining
OT  - Microscopic imaging analysis
OT  - Point-of-care testing
OT  - Somatic cell counting
EDAT- 2023/06/29 13:42
MHDA- 2023/08/23 06:42
CRDT- 2023/06/29 11:05
PHST- 2023/05/07 00:00 [received]
PHST- 2023/06/16 00:00 [accepted]
PHST- 2023/06/15 00:00 [revised]
PHST- 2023/08/23 06:42 [medline]
PHST- 2023/06/29 13:42 [pubmed]
PHST- 2023/06/29 11:05 [entrez]
AID - 10.1007/s00216-023-04823-3 [pii]
AID - 10.1007/s00216-023-04823-3 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2023 Sep;415(22):5499-5509. doi: 10.1007/s00216-023-04823-3. 
      Epub 2023 Jun 29.