PMID- 37385291
OWN - NLM
STAT- Publisher
LR  - 20230629
IS  - 1752-699X (Electronic)
IS  - 1752-6981 (Linking)
DP  - 2023 Jun 29
TI  - Expression patterns of serum miR-27a-3p and activating transcription factor 3 in 
      children with bronchial asthma and their correlations with airway inflammation.
LID - 10.1111/crj.13631 [doi]
AB  - INTRODUCTION: Bronchial asthma (BA) is a heterogeneous disease characterized by 
      chronic airway inflammation. This study investigated the serum 
      miR-27a-3p/activating transcription factor 3 (ATF3) expression in children with 
      BA and their correlations with airway inflammation. METHODS: Children with BA 
      (N = 120) and healthy children (N = 108) were enrolled. Serum levels of 
      interleukin (IL)-17, IL-6, tumor necrosis factor (TNF)-alpha, immunoglobulin E (IgE), 
      miR-27a-3p, ATF3, and the number of eosinophils (EOS) were measured using 
      enzyme-linked immunosorbent assay (ELISA), reverse transcription quantitative 
      polymerase chain reaction (RT-qPCR), and an automatic hematology analyzer. The 
      correlations between miR-27a-3p and ATF3 and between miR-27a-3p/ATF3 and 
      inflammation-related factors were analyzed by the Pearson method. The diagnostic 
      values of miR-27a-3p and ATF3 in BA were evaluated using receiver operating 
      characteristic (ROC) curves. The influencing factors of BA were assessed using 
      multivariate logistic regression. Finally, the targeting relation between 
      miR-27a-3p and ATF3 was predicted and analyzed by TargetScan and Starbase 
      databases, and dual-luciferase assay. RESULTS: There were significant differences 
      in forced expiratory volume in 1 s (FEV1)% predicted and FEV1/forced vital 
      capacity (FVC)%, serum levels of IgE, IL-17, IL-6, and TNF-alpha, and EOS numbers 
      between healthy children and BA children. Serum miR-27a-3p was negatively 
      correlated with ATF3 and positively correlated with inflammation-related factors 
      in BA children. Serum ATF3 mRNA levels were negatively correlated with 
      inflammatory factors in BA children. miR-27a-3p and ATF3 had good diagnostic 
      values in BA children. FEV% predicted, IL-6, TNF-alpha, miR-27a-3p, and ATF3 were 
      independent risk factors for BA. miR-27a-3p targeted ATF3. CONCLUSION: Serum 
      miR-27a-3p was highly expressed, whereas ATF3 was poorly expressed in BA 
      children, and they were significantly correlated with airway inflammation, had 
      good diagnostic values in BA children, and were independent risk factors for 
      asthma.
CI  - (c) 2023 The Authors. The Clinical Respiratory Journal published by John Wiley & 
      Sons Ltd.
FAU - Wang, Jingcai
AU  - Wang J
AD  - Department of Pediatric, The Affiliated Hospital of Chengde Medical University, 
      Chengde, Hebei, China.
FAU - Yang, Lixin
AU  - Yang L
AD  - Department of Pediatric, The Affiliated Hospital of Chengde Medical University, 
      Chengde, Hebei, China.
FAU - Sun, Peng
AU  - Sun P
AD  - Department of Pediatric, The Affiliated Hospital of Chengde Medical University, 
      Chengde, Hebei, China.
FAU - Guo, Chunyan
AU  - Guo C
AD  - Department of Pediatric, The Affiliated Hospital of Chengde Medical University, 
      Chengde, Hebei, China.
FAU - Jin, Yuzi
AU  - Jin Y
AD  - Department of Pediatric, The Affiliated Hospital of Chengde Medical University, 
      Chengde, Hebei, China.
FAU - Jing, Xiaoqing
AU  - Jing X
AUID- ORCID: 0000-0001-7260-3837
AD  - Department of Pediatric, The Affiliated Hospital of Chengde Medical University, 
      Chengde, Hebei, China.
LA  - eng
PT  - Journal Article
DEP - 20230629
PL  - England
TA  - Clin Respir J
JT  - The clinical respiratory journal
JID - 101315570
SB  - IM
OTO - NOTNLM
OT  - IL-17
OT  - IL-6
OT  - TNF-alpha
OT  - activating transcription factor 3
OT  - airway inflammation
OT  - bronchial asthma
OT  - eosinophils
OT  - miR-27a-3p
EDAT- 2023/06/30 01:06
MHDA- 2023/06/30 01:06
CRDT- 2023/06/29 18:53
PHST- 2023/03/30 00:00 [revised]
PHST- 2022/12/21 00:00 [received]
PHST- 2023/05/05 00:00 [accepted]
PHST- 2023/06/30 01:06 [medline]
PHST- 2023/06/30 01:06 [pubmed]
PHST- 2023/06/29 18:53 [entrez]
AID - 10.1111/crj.13631 [doi]
PST - aheadofprint
SO  - Clin Respir J. 2023 Jun 29. doi: 10.1111/crj.13631.