PMID- 37385819
OWN - NLM
STAT- MEDLINE
DCOM- 20230726
LR  - 20230726
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 61
IP  - 7
DP  - 2023 Jul 6
TI  - Opaque cell-specific proteome of Candida albicans ATCC 10231.
LID - myad062 [pii]
LID - 10.1093/mmy/myad062 [doi]
AB  - Candida albicans, a polymorphic opportunistic pathogen of humans, can exist in 
      different morphological forms like yeast, hyphae, pseudohyphae, chlamydospores, 
      and white and opaque cells. Proteomic analysis of opaque form of C. albicans ATCC 
      10231 is carried out in the present study using microflow liquid 
      chromatography-tandem mass spectrometry and validated using expression analysis 
      of selected genes using reverse transcription quantitative real-time PCR and 
      mitochondrial membrane potential assay. This is the first report identifying 
      opaque cell-specific proteins of C. albicans. A total of 188 proteins were 
      significantly modulated under opaque form compared to white cells, of which 110 
      were upregulated, and 78 were downregulated. It was observed that oxidative 
      phosphorylation (OxPhos) and oxidative stress are enhanced in C. albicans cells 
      growing under opaque form as proteins involved in OxPhos (Atp1, Atp3, Atp16, 
      Atp7, Cox6, Nuc2, Qcr7, and Sdh12) and oxidative stress response (Gcs1, Gtt11, 
      Gpx2, Sod1, Ccp1, and Lys7) were significantly upregulated. The maximum 
      upregulation of 23.16- and 13.93-fold is observed in the cases of Ccp1 and Nuc2, 
      respectively. The downregulation of proteins, namely Als1, Csh1, Sap9, and Rho1, 
      determining cell surface chemistry indicates modulation in cell wall integrity 
      and reduced adhesion of opaque cells compared to white cells. This study is 
      significant as it is the first draft of the proteomic profile of opaque cells 
      that suggests enhanced OxPhos, oxidative stress, and modulation in cell surface 
      chemistry indicating reduced adhesion and cell wall integrity, which could be 
      associated with reduced virulence in opaque form. However, a deeper investigation 
      is needed to explore it further.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of The 
      International Society for Human and Animal Mycology.
FAU - Abdulghani, Mazen
AU  - Abdulghani M
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 
      Maharashtra, India.
FAU - Telang, Saraswati
AU  - Telang S
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 
      Maharashtra, India.
FAU - Desai, Manisha
AU  - Desai M
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 
      Maharashtra, India.
FAU - Kadam, Shivdas
AU  - Kadam S
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 
      Maharashtra, India.
FAU - Kazi, Rubina
AU  - Kazi R
AD  - Division of Biochemical Sciences, CSIR-NCL, Pune, Maharshtra, India.
FAU - Shelar, Amruta
AU  - Shelar A
AD  - Department of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune, 
      Maharashtra, India.
FAU - Patil, Rajendra
AU  - Patil R
AD  - Department of Biotechnology, Savitribai Phule Pune University, Ganeshkhind, Pune, 
      Maharashtra, India.
FAU - Maurya, Radheshyam
AU  - Maurya R
AD  - School of Life Sciences, University of Hyderabad, Gachibowli, Telangana, India.
FAU - Zore, Gajanan
AU  - Zore G
AUID- ORCID: 0000-0001-9213-6164
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 
      Maharashtra, India.
LA  - eng
GR  - SRTM University/
GR  - SAP-DRS II/UGC/
GR  - DST FIST I/DST/
PT  - Journal Article
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
RN  - 0 (Fungal Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Humans
MH  - Animals
MH  - *Candida albicans/genetics
MH  - *Fungal Proteins/genetics
MH  - Proteome/genetics/metabolism
MH  - Proteomics
MH  - Oxidative Phosphorylation
MH  - Gene Expression Regulation, Fungal
OAB - Opaque form is one of the least studied morphological forms of Candida albicans. 
      To the best of our knowledge, this is the first report providing opaque 
      cell-specific proteome. It suggests enhanced oxidative phosphorylation, oxidative 
      stress, and modulation in cell surface chemistry, which could be associated with 
      reduced virulence in opaque form.
OABL- eng
OTO - NOTNLM
OT  - C. albicans
OT  - LC-MS/MS
OT  - OxPhos
OT  - opaque
OT  - oxidative stress
OT  - protein
EDAT- 2023/06/30 01:06
MHDA- 2023/07/26 06:43
CRDT- 2023/06/29 21:52
PHST- 2023/02/07 00:00 [received]
PHST- 2023/06/17 00:00 [revised]
PHST- 2023/06/28 00:00 [accepted]
PHST- 2023/07/26 06:43 [medline]
PHST- 2023/06/30 01:06 [pubmed]
PHST- 2023/06/29 21:52 [entrez]
AID - 7210534 [pii]
AID - 10.1093/mmy/myad062 [doi]
PST - ppublish
SO  - Med Mycol. 2023 Jul 6;61(7):myad062. doi: 10.1093/mmy/myad062.