PMID- 37387267
OWN - NLM
STAT- MEDLINE
DCOM- 20230712
LR  - 20230718
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 95
IP  - 27
DP  - 2023 Jul 11
TI  - Wash-Free and High-Contrast Imaging of Single-Nucleotide Variation in Single 
      Cells Using Transcription-Amplified Light-Up RNA Aptamer.
PG  - 10163-10171
LID - 10.1021/acs.analchem.3c00089 [doi]
AB  - Single-nucleotide variation (SNV) imaging can indicate cellular heterogeneity and 
      spatial pattern, but it remains challenging to produce high-gain signal while 
      also yielding single-nucleotide resolution. Herein, we developed a light-up 
      strategy for visualizing SNVs based on transcription amplification, enabling 
      wash-free and high-contrast imaging of SNVs inside cells. The discrimination of 
      SNVs is achieved by ligase-assisted transcription reaction. Employing a light-up 
      RNA aptamer as a reporter eliminates nonspecific probe binding and the washing 
      process and contributes to a 2-fold improvement of signal gain compared to that 
      using the fluorescence in situ hybridization (FISH) method. The method allowed us 
      to precisely quantify drug-resistant strains in the bacteria mixture and identify 
      drug-resistant Salmonella enterica (S. enterica) isolated from poultry farm. 
      Using this approach, we explored the colonization features of drug-resistant and 
      drug-sensitive S. enterica in the mice intestinal tract and screened the 
      prebiotics for Salmonella colonization inhibition. The SNV imaging method 
      promises for the interrogation of genotypes in physiological and pathological 
      states at the single-cell level.
FAU - Xia, Xuhan
AU  - Xia X
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - Li, Feng
AU  - Li F
AUID- ORCID: 0000-0002-2616-5343
AD  - College of Chemistry, Analytical & Testing Centre, Sichuan University, Chengdu, 
      Sichuan 610064, China.
FAU - Zhang, Yong
AU  - Zhang Y
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - Zhu, Yulin
AU  - Zhu Y
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - Song, Sishuo
AU  - Song S
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - He, Guiping
AU  - He G
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - Zhang, Jiaqi
AU  - Zhang J
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - He, Qiang
AU  - He Q
AUID- ORCID: 0000-0001-5901-5627
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
FAU - Deng, Ruijie
AU  - Deng R
AUID- ORCID: 0000-0001-9094-124X
AD  - College of Biomass Science and Engineering, Healthy Food Evaluation Research 
      Center, Sichuan University, Chengdu 610065, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230630
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Aptamers, Nucleotide/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Salmonella/genetics
MH  - *Salmonella enterica/genetics
MH  - Diagnostic Imaging
EDAT- 2023/06/30 06:42
MHDA- 2023/07/12 06:42
CRDT- 2023/06/30 05:43
PHST- 2023/07/12 06:42 [medline]
PHST- 2023/06/30 06:42 [pubmed]
PHST- 2023/06/30 05:43 [entrez]
AID - 10.1021/acs.analchem.3c00089 [doi]
PST - ppublish
SO  - Anal Chem. 2023 Jul 11;95(27):10163-10171. doi: 10.1021/acs.analchem.3c00089. 
      Epub 2023 Jun 30.