PMID- 37388775
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230703
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 11
DP  - 2023
TI  - Baseline investigation on soil solidification through biocementation using 
      airborne bacteria.
PG  - 1216171
LID - 10.3389/fbioe.2023.1216171 [doi]
LID - 1216171
AB  - Microbial induced carbonate precipitation (MICP) through the ureolysis metabolic 
      pathway is one of the most studied topics in biocementation due to its high 
      efficiency. Although excellent outcomes have proved the potential of this 
      technique, microorganisms face some obstacles when considering complicated 
      situations in the real field, such as bacterial adaptability and survivability 
      issues. This study made the first attempt to seek solutions to this issue from 
      the air, exploring ureolytic airborne bacteria with resilient features to find a 
      solution to survivability issues. Samples were collected using an air sampler in 
      Sapporo, Hokkaido, a cold region where sampling sites were mostly covered with 
      dense vegetation. After two rounds of screening, 12 out of 57 urease-positive 
      isolates were identified through 16S rRNA gene analysis. Four potentially 
      selected strains were then evaluated in terms of growth pattern and activity 
      changes within a range of temperatures (15 degrees C-35 degrees C). The results from sand 
      solidification tests using two Lederbergia strains with the best performance 
      among the isolates showed an improvement in unconfined compressive strength up to 
      4-8 MPa after treatment, indicating a high MICP efficiency. Overall, this 
      baseline study demonstrated that the air could be an ideal isolation source for 
      ureolytic bacteria and laid a new pathway for MICP applications. More 
      investigations on the performance of airborne bacteria under changeable 
      environments may be required to further examine their survivability and 
      adaptability.
CI  - Copyright (c) 2023 Chen, Gowthaman, Nakashima, Takano and Kawasaki.
FAU - Chen, Meiqi
AU  - Chen M
AD  - Laboratory of Biotechnology for Resources Engineering, Graduate School of 
      Engineering, Hokkaido University, Sapporo, Japan.
FAU - Gowthaman, Sivakumar
AU  - Gowthaman S
AD  - Department of Engineering Technology, Faculty of Technology, University of 
      Jaffna, Kilinochchi, Sri Lanka.
FAU - Nakashima, Kazunori
AU  - Nakashima K
AD  - Laboratory of Biotechnology for Resources Engineering, Faculty of Engineering, 
      Hokkaido University, Sapporo, Japan.
FAU - Takano, Chikara
AU  - Takano C
AD  - Laboratory of Biotechnology for Resources Engineering, Faculty of Engineering, 
      Hokkaido University, Sapporo, Japan.
FAU - Kawasaki, Satoru
AU  - Kawasaki S
AD  - Laboratory of Biotechnology for Resources Engineering, Faculty of Engineering, 
      Hokkaido University, Sapporo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20230614
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC10300444
OTO - NOTNLM
OT  - airborne bacteria
OT  - bacterial identification
OT  - microbial induced carbonate precipitation
OT  - unconfined compressive strength
OT  - urease activity
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/06/30 13:11
MHDA- 2023/06/30 13:12
PMCR- 2023/01/01
CRDT- 2023/06/30 10:08
PHST- 2023/05/03 00:00 [received]
PHST- 2023/06/02 00:00 [accepted]
PHST- 2023/06/30 13:12 [medline]
PHST- 2023/06/30 13:11 [pubmed]
PHST- 2023/06/30 10:08 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1216171 [pii]
AID - 10.3389/fbioe.2023.1216171 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2023 Jun 14;11:1216171. doi: 10.3389/fbioe.2023.1216171. 
      eCollection 2023.