PMID- 37391416
OWN - NLM
STAT- MEDLINE
DCOM- 20230703
LR  - 20231117
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 14
IP  - 1
DP  - 2023 Jun 30
TI  - Targeted cross-linker delivery for the in situ mapping of protein conformations 
      and interactions in mitochondria.
PG  - 3882
LID - 10.1038/s41467-023-39485-3 [doi]
LID - 3882
AB  - Current methods for intracellular protein analysis mostly require the separation 
      of specific organelles or changes to the intracellular environment. However, the 
      functions of proteins are determined by their native microenvironment as they 
      usually form complexes with ions, nucleic acids, and other proteins. Here, we 
      show a method for in situ cross-linking and analysis of mitochondrial proteins in 
      living cells. By using the poly(lactic-co-glycolic acid) (PLGA) nanoparticles 
      functionalized with dimethyldioctadecylammonium bromide (DDAB) to deliver protein 
      cross-linkers into mitochondria, we subsequently analyze the cross-linked 
      proteins using mass spectrometry. With this method, we identify a total of 74 
      pairs of protein-protein interactions that do not exist in the STRING database. 
      Interestingly, our data on mitochondrial respiratory chain proteins ( ~ 94%) are 
      also consistent with the experimental or predicted structural analysis of these 
      proteins. Thus, we provide a promising technology platform for in situ defining 
      protein analysis in cellular organelles under their native microenvironment.
CI  - (c) 2023. The Author(s).
FAU - Chen, Yuwan
AU  - Chen Y
AUID- ORCID: 0000-0002-0844-1762
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Zhou, Wen
AU  - Zhou W
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Xia, Yufei
AU  - Xia Y
AUID- ORCID: 0000-0003-1215-6128
AD  - State Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
FAU - Zhang, Weijie
AU  - Zhang W
AUID- ORCID: 0000-0001-5720-3130
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Zhao, Qun
AU  - Zhao Q
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
FAU - Li, Xinwei
AU  - Li X
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
AD  - Zhang Dayu School of Chemistry, Dalian University of Technology, Dalian, 116024, 
      China.
FAU - Gao, Hang
AU  - Gao H
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Liang, Zhen
AU  - Liang Z
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
FAU - Ma, Guanghui
AU  - Ma G
AUID- ORCID: 0000-0001-9154-5556
AD  - State Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
FAU - Yang, Kaiguang
AU  - Yang K
AUID- ORCID: 0000-0001-9544-2714
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China. yangkaiguang@dicp.ac.cn.
FAU - Zhang, Lihua
AU  - Zhang L
AUID- ORCID: 0000-0003-3798-2047
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China. lihuazhang@dicp.ac.cn.
FAU - Zhang, Yukui
AU  - Zhang Y
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, 
      China.
LA  - eng
SI  - figshare/10.6084/m9.figshare.23261618
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230630
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Glycols)
SB  - IM
MH  - *Mitochondria
MH  - Protein Conformation
MH  - *Mitochondrial Membranes
MH  - Databases, Factual
MH  - Glycols
PMC - PMC10313818
COIS- The authors declare no competing interests.
EDAT- 2023/07/01 11:41
MHDA- 2023/07/03 06:41
PMCR- 2023/06/30
CRDT- 2023/06/30 23:16
PHST- 2022/06/15 00:00 [received]
PHST- 2023/06/15 00:00 [accepted]
PHST- 2023/07/03 06:41 [medline]
PHST- 2023/07/01 11:41 [pubmed]
PHST- 2023/06/30 23:16 [entrez]
PHST- 2023/06/30 00:00 [pmc-release]
AID - 10.1038/s41467-023-39485-3 [pii]
AID - 39485 [pii]
AID - 10.1038/s41467-023-39485-3 [doi]
PST - epublish
SO  - Nat Commun. 2023 Jun 30;14(1):3882. doi: 10.1038/s41467-023-39485-3.