PMID- 37391517
OWN - NLM
STAT- MEDLINE
DCOM- 20230703
LR  - 20231124
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Jun 30
TI  - Seasonal prevalence of bacteria in the outflow of two full-scale municipal 
      wastewater treatment plants.
PG  - 10608
LID - 10.1038/s41598-023-37744-3 [doi]
LID - 10608
AB  - Despite many modern wastewater treatment solutions, the most common is still the 
      use of activated sludge (AS). Studies indicate that the microbial composition of 
      AS is most often influenced by the raw sewage composition (especially influent 
      ammonia), biological oxygen demand, the level of dissolved oxygen, technological 
      solutions, as well as the temperature of wastewater related to seasonality. The 
      available literature mainly refers to the relationship between AS parameters or 
      the technology used and the composition of microorganisms in AS. However, there 
      is a lack of data on the groups of microorganisms leaching into water bodies 
      whose presence is a signal for possible changes in treatment technology. 
      Moreover, sludge flocs in the outflow contain less extracellular substance (EPS) 
      which interferes microbial identification. The novelty of this article concerns 
      the identification and quantification of microorganisms in the AS and in the 
      outflow by fluorescence in situ hybridization (FISH) method from two full-scale 
      wastewater treatment plants (WWTPs) in terms of 4 key groups of microorganisms 
      involved in the wastewater treatment process in the context of their potential 
      technological usefulness. The results of the study showed that Nitrospirae, 
      Chloroflexi and Ca. Accumulibacter phosphatis in treated wastewater reflect the 
      trend in abundance of these bacteria in activated sludge. Increased abundance of 
      betaproteobacterial ammonia-oxidizing bacteria and Nitrospirae in the outflow 
      were observed in winter. Principal component analysis (PCA) showed that loadings 
      obtained from abundance of bacteria in the outflow made larger contributions to 
      the variance in the PC1 factorial axis, than loadings obtained from abundance of 
      bacteria from activated sludge. PCA confirmed the reasonableness of conducting 
      studies not only in the activated sludge, but also in the outflow to find 
      correlations between technological problems and qualitative and quantitative 
      changes in the outflow microorganisms.
CI  - (c) 2023. The Author(s).
FAU - Domanska, Magdalena
AU  - Domanska M
AD  - Institute of Environmental Engineering, Wroclaw University of Environmental and 
      Life Sciences, pl. Grunwaldzki 24, 50-363, Wroclaw, Poland. 
      magdalena.domanska@upwr.edu.pl.
FAU - Kusnierz, Magdalena
AU  - Kusnierz M
AD  - Institute of Environmental Engineering, Wroclaw University of Environmental and 
      Life Sciences, pl. Grunwaldzki 24, 50-363, Wroclaw, Poland.
FAU - Mackiewicz, Katarzyna
AU  - Mackiewicz K
AD  - Institute of Environmental Engineering, Wroclaw University of Environmental and 
      Life Sciences, pl. Grunwaldzki 24, 50-363, Wroclaw, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230630
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Sewage)
RN  - 0 (Wastewater)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - *Sewage
MH  - Wastewater
MH  - Ammonia
MH  - In Situ Hybridization, Fluorescence
MH  - Prevalence
MH  - Seasons
MH  - Bacteria/genetics
MH  - *Betaproteobacteria
PMC - PMC10313732
COIS- The authors declare no competing interests.
EDAT- 2023/07/01 11:41
MHDA- 2023/07/03 06:41
PMCR- 2023/06/30
CRDT- 2023/06/30 23:24
PHST- 2023/04/04 00:00 [received]
PHST- 2023/06/27 00:00 [accepted]
PHST- 2023/07/03 06:41 [medline]
PHST- 2023/07/01 11:41 [pubmed]
PHST- 2023/06/30 23:24 [entrez]
PHST- 2023/06/30 00:00 [pmc-release]
AID - 10.1038/s41598-023-37744-3 [pii]
AID - 37744 [pii]
AID - 10.1038/s41598-023-37744-3 [doi]
PST - epublish
SO  - Sci Rep. 2023 Jun 30;13(1):10608. doi: 10.1038/s41598-023-37744-3.