PMID- 37391581
OWN - NLM
STAT- MEDLINE
DCOM- 20230703
LR  - 20231124
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Jun 30
TI  - The effects of IL-1beta stimulated human umbilical cord mesenchymal stem cells on 
      polarization and apoptosis of macrophages in rheumatoid arthritis.
PG  - 10612
LID - 10.1038/s41598-023-37741-6 [doi]
LID - 10612
AB  - Macrophages play an important role in the pathogenesis of rheumatoid arthritis 
      (RA), in which the functions of pro-inflammatory macrophages (M1) and 
      anti-inflammatory macrophages (M2) are different. Our previous studies have 
      demonstrated that interleukin-1beta (IL-1beta) stimulated human umbilical cord 
      mesenchymal stem cells (hUCMSCs) increase the expression of tumor necrosis 
      factor-related apoptosis-inducing ligand (TRAIL) and initiate breast cancer cell 
      apoptosis via ligand to death receptor 4 (DR4) and DR5. In this study, we 
      examined the effect of IL-1beta stimulated hUCMSCs (IL-1beta-hUCMSCs) on 
      immunoregulation of M1 and M2 macrophages in vitro and in the RA mouse model. The 
      results showed that IL-1beta-hUCMSCs increased macrophage polarization into M2 
      macrophages and enhanced apoptosis of M1 macrophages in vitro. Moreover, the 
      intravenous injected IL-1beta-hUCMSCs in RA mice rehabilitated the imbalance of 
      M1/M2 ratio and thus demonstrated the potential to reduce inflammation in RA. 
      This study advances our knowledge of the underlying immunoregulatory mechanisms 
      involved in IL-1beta-hUCMSCs to induce M1 macrophage apoptosis and promote the 
      anti-inflammatory polarization of M2 macrophages and demonstrates the potential 
      of IL-1beta-hUCMSCs to reduce inflammation in RA.
CI  - (c) 2023. The Author(s).
FAU - Zeng, Ying-Xuan
AU  - Zeng YX
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Peitou, Taipei, 112, Taiwan, ROC.
FAU - Chou, Kuang-Yi
AU  - Chou KY
AD  - School of Nursing, National Taipei University of Nursing and Health Sciences, 
      Taipei, Taiwan, ROC.
FAU - Hwang, Jeng-Jong
AU  - Hwang JJ
AD  - Department of Medical Imaging, Department of Medical Imaging and Radiological 
      Sciences, Chung Shan Medical University Hospital, Chung Shan Medical University, 
      Taichung, Taiwan, ROC.
FAU - Wang, Hwai-Shi
AU  - Wang HS
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Peitou, Taipei, 112, Taiwan, ROC. hswang@nycu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230630
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Interleukin-1beta)
RN  - 0 (Ligands)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - Interleukin-1beta
MH  - Ligands
MH  - *Arthritis, Rheumatoid/therapy
MH  - Inflammation
MH  - Apoptosis
MH  - Macrophages
MH  - *Mesenchymal Stem Cells
MH  - Tumor Necrosis Factor-alpha
MH  - Umbilical Cord
PMC - PMC10313744
COIS- The authors declare that a patent application entitled "Composition and Use of 
      Interleukin Stimulated Human Umbilical Cord Mesenchymal Stem Cells for the 
      Treatment of Rheumatoid Arthritis" granted in Taiwan in 26-July-2022 was part of 
      the research in this paper. Based on part of the research in this paper, an US 
      patent application of the same title was filed on 8-JUN-2021.
EDAT- 2023/07/01 11:41
MHDA- 2023/07/03 06:41
PMCR- 2023/06/30
CRDT- 2023/06/30 23:28
PHST- 2023/04/18 00:00 [received]
PHST- 2023/06/27 00:00 [accepted]
PHST- 2023/07/03 06:41 [medline]
PHST- 2023/07/01 11:41 [pubmed]
PHST- 2023/06/30 23:28 [entrez]
PHST- 2023/06/30 00:00 [pmc-release]
AID - 10.1038/s41598-023-37741-6 [pii]
AID - 37741 [pii]
AID - 10.1038/s41598-023-37741-6 [doi]
PST - epublish
SO  - Sci Rep. 2023 Jun 30;13(1):10612. doi: 10.1038/s41598-023-37741-6.