PMID- 37392526
OWN - NLM
STAT- MEDLINE
DCOM- 20230814
LR  - 20230814
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 287
DP  - 2023 Aug
TI  - Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in 
      Brassica napus.
PG  - 154032
LID - S0176-1617(23)00126-8 [pii]
LID - 10.1016/j.jplph.2023.154032 [doi]
AB  - To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the 
      oil-producing crop Brassica napus L., we generated transgenic plants in which 
      BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter. 
      Over-expression of BnPgb2 elevated the amount of oil, which showed a positive 
      relationship with the level of BnPgb2, without altering the oil nutritional 
      value, as evidenced by the lack of major changes in composition of fatty acids 
      (FA), and key agronomic traits. Two key transcription factors, LEAFY COTYLEDON1 
      (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) 
      and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds. 
      The concomitant induction of several enzymes of sucrose metabolism, SUCROSE 
      SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and 
      PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE 
      (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production. The 
      two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), 
      and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the 
      over-expression of BnPgb2. The requirement of BnPgb2 for oil deposition was 
      further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of 
      high-oil genotypes relative to their low-oil counterparts.
CI  - Copyright (c) 2023 Elsevier GmbH. All rights reserved.
FAU - Haq, Md Ehsanul
AU  - Haq ME
AD  - Department of Plant Science, University of Manitoba, Winnipeg, R3T2Z2, MB, 
      Canada.
FAU - Mira, Mohammed M
AU  - Mira MM
AD  - Department of Plant Science, University of Manitoba, Winnipeg, R3T2Z2, MB, 
      Canada.
FAU - Duncan, Robert W
AU  - Duncan RW
AD  - Department of Plant Science, University of Manitoba, Winnipeg, R3T2Z2, MB, 
      Canada.
FAU - Hill, Robert D
AU  - Hill RD
AD  - Department of Plant Science, University of Manitoba, Winnipeg, R3T2Z2, MB, 
      Canada.
FAU - Stasolla, Claudio
AU  - Stasolla C
AD  - Department of Plant Science, University of Manitoba, Winnipeg, R3T2Z2, MB, 
      Canada. Electronic address: Claudio.stasolla@umanitoba.ca.
LA  - eng
PT  - Journal Article
DEP - 20230614
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Fatty Acids)
RN  - 0 (Plant Oils)
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - *Brassica napus/genetics/metabolism
MH  - Fatty Acids/metabolism
MH  - Seeds/genetics/metabolism
MH  - Plants, Genetically Modified/genetics/metabolism
MH  - Plant Oils/metabolism
MH  - Sucrose/metabolism
MH  - Gene Expression Regulation, Plant
OTO - NOTNLM
OT  - Brassica napus
OT  - Fatty acids
OT  - LEAFY COTYLEDON1
OT  - Phytoglobin
OT  - Seed oil
OT  - WRINKLED1
COIS- Declaration of competing interest There are no financial and personal 
      relationships with other people or organizations that could inappropriately 
      influence (bias) their work.
EDAT- 2023/07/02 01:10
MHDA- 2023/08/14 06:41
CRDT- 2023/07/01 18:02
PHST- 2023/04/10 00:00 [received]
PHST- 2023/06/06 00:00 [revised]
PHST- 2023/06/07 00:00 [accepted]
PHST- 2023/08/14 06:41 [medline]
PHST- 2023/07/02 01:10 [pubmed]
PHST- 2023/07/01 18:02 [entrez]
AID - S0176-1617(23)00126-8 [pii]
AID - 10.1016/j.jplph.2023.154032 [doi]
PST - ppublish
SO  - J Plant Physiol. 2023 Aug;287:154032. doi: 10.1016/j.jplph.2023.154032. Epub 2023 
      Jun 14.