PMID- 37392778
OWN - NLM
STAT- MEDLINE
DCOM- 20230724
LR  - 20230724
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 328
DP  - 2023 Sep 1
TI  - Effect of Au@SiO(2) core shell nanoparticles on HG-induced oxidative stress 
      triggered apoptosis in keratinocytes.
PG  - 121893
LID - S0024-3205(23)00528-3 [pii]
LID - 10.1016/j.lfs.2023.121893 [doi]
AB  - Growing evidences suggest that excess generation of highly reactive free 
      oxygen/nitrogen radicals (ROS/RNS) are largely due to hyperglycemia causes 
      oxidative stress. Further, excess accumulation of ROS/RNS in cellular 
      compartments aggravates the development and progression of diabetes and its 
      associated complications. Impaired wound healing in diabetic condition is a known 
      vital complication all around the world. Thus, an antioxidant agent having the 
      potential for hindering the oxidative/nitrosative stress triggered diabetic skin 
      complication is required. The present investigation was carried out to understand 
      the impact of silica coated gold nanoparticle (Au@SiO(2) NPs) on high glucose 
      (HG)-induced keratinocyte complications. We demonstrated that HG environment 
      enhanced the ROS and RNS accumulations and reduced in cellular antioxidant 
      capacities in keratinocte cells, however, Au@SiO(2) NPs treatment restored the HG 
      effect. Furthermore, excess production of ROS/RNS was associated with 
      mitochondrial dysfunction, characterized by loss of mitochondrial membrane 
      potential (DeltaPsim), and increased in mitochondrial mass, which was restored by 
      Au@SiO(2) NPs treatment in keratinocyte cells. In addition, HG-induced excess 
      production of ROS/RNA caused an increased in the biomolecules damage including 
      lipid peroxidation (LPO), and protein carbonylation (PC), 8-oxoguanine DNA 
      glycosylase-1 (OGG1) expression and increased 8-hydroxydeoxyguanosine (8-OHdG) 
      accumulations in DNA, leading to activation of ERK1/2MAPK, AKT and tuberin 
      pathway, inflammatory reaction, and finally apoptotic cell death. In conclusion, 
      our findings showed that Au@SiO(2) NPs treatment improved the HG-induced 
      keratinocytes injury by suppressing the oxidative/nitrosative stress, elevating 
      the antioxidant defence system, thereby inhibiting the inflammatory mediators and 
      apoptosis, which may be a therapeutic cure for the diabetic keratinocyte 
      problems.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Rizwan, Huma
AU  - Rizwan H
AD  - School of Biotechnology, KIIT Deemed to be University, Bhubaneswar 751024, India.
FAU - Satapathy, Smith Sagar
AU  - Satapathy SS
AD  - School of Chemical Technology, KIIT Deemed to be University, Bhubaneswar 751024, 
      India.
FAU - Si, Satyabrata
AU  - Si S
AD  - School of Biotechnology, KIIT Deemed to be University, Bhubaneswar 751024, India; 
      School of Chemical Technology, KIIT Deemed to be University, Bhubaneswar 751024, 
      India.
FAU - Kumar, Sonu
AU  - Kumar S
AD  - Department of Zoology, School of Life Sciences, Mahatma Gandhi Central 
      University, Motihari, Bihar 845401, India.
FAU - Kumari, Golden
AU  - Kumari G
AD  - Department of Zoology, School of Life Sciences, Mahatma Gandhi Central 
      University, Motihari, Bihar 845401, India.
FAU - Pal, Arttatrana
AU  - Pal A
AD  - School of Biotechnology, KIIT Deemed to be University, Bhubaneswar 751024, India; 
      Department of Zoology, School of Life Sciences, Mahatma Gandhi Central 
      University, Motihari, Bihar 845401, India. Electronic address: 
      arttatranapal@mgcub.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20230629
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Humans
MH  - Antioxidants/pharmacology/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Silicon Dioxide/toxicity/metabolism
MH  - Gold/pharmacology
MH  - Signal Transduction
MH  - *Metal Nanoparticles/toxicity
MH  - Oxidative Stress
MH  - *Diabetes Mellitus/metabolism
MH  - Keratinocytes/metabolism
MH  - Apoptosis
MH  - *Nanoparticles
OTO - NOTNLM
OT  - Apoptosis
OT  - Au@SiO(2) NPs
OT  - Biomolecules damage
OT  - High glucose
OT  - Keratinocytes
OT  - Oxidative/nitrosative stress
COIS- Declaration of competing interest All authors have declared that no potential 
      conflicts of interest relevant to this article were reported.
EDAT- 2023/07/02 01:10
MHDA- 2023/07/24 06:42
CRDT- 2023/07/01 19:11
PHST- 2023/03/20 00:00 [received]
PHST- 2023/06/17 00:00 [revised]
PHST- 2023/06/25 00:00 [accepted]
PHST- 2023/07/24 06:42 [medline]
PHST- 2023/07/02 01:10 [pubmed]
PHST- 2023/07/01 19:11 [entrez]
AID - S0024-3205(23)00528-3 [pii]
AID - 10.1016/j.lfs.2023.121893 [doi]
PST - ppublish
SO  - Life Sci. 2023 Sep 1;328:121893. doi: 10.1016/j.lfs.2023.121893. Epub 2023 Jun 
      29.