PMID- 37395518
OWN - NLM
STAT- MEDLINE
DCOM- 20230704
LR  - 20230714
IS  - 1867-5646 (Electronic)
IS  - 1462-6446 (Linking)
VI  - 26
IP  - 2
DP  - 2023 Jun 23
TI  - Small Molecule Nitazoxanide Inhibits Osteogenic Differentiation and Promotes 
      Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells.
PG  - 69-75
LID - 10.3290/j.cjdr.b4128019 [doi]
AB  - OBJECTIVE: To investigate the potential effect of small molecule nitazoxanide 
      (NTZ) on the osteogenic and adipogenic differentiation of bone marrow mesenchymal 
      stem cells (BMSCs). METHODS: Cell counting Kit-8 assay was used to examine the 
      effect of NTZ on proliferation of BMSCs. Quantitative reverse transcription 
      polymerase chain reaction (qRT-PCR) and Western blot analysis were used to 
      measure the expression of osteogenic and adipogenic marker gene. Alkaline 
      phosphatase (ALP) staining and activity assay and Alizarin Red S (ARS) staining 
      were used to investigate the effect of NTZ on osteogenesis. Oil red O (ORO) 
      staining assay was used to assess the impact of NTZ on adipogenesis. RESULTS: NTZ 
      significantly suppressed the osteogenic differentiation but promoted the 
      adipogenic differentiation of BMSCs. Mechanistically, NTZ regulated 
      osteogenic/adipogenic differentiation of BMSCs by inhibiting the Wnt/beta-catenin 
      signalling pathway. The addition of Wnt/beta-catenin signalling pathway activator, 
      lithium chloride, could reverse the effect of NTZ on BMSCs. CONCLUSION: NTZ 
      affected osteogenic and adipogenic differentiation of BMSCs with the involvement 
      of Wnt/beta-catenin signalling pathway. This finding expanded the understanding of 
      NTZ pharmacology and indicated that NTZ might have an adverse effect on bone 
      homeostasis.
FAU - Jin, Chan Yuan
AU  - Jin CY
FAU - Guo, Yan Yan
AU  - Guo YY
FAU - Hou, Xiao Mei
AU  - Hou XM
FAU - Tang, Zhi Hui
AU  - Tang ZH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Chin J Dent Res
JT  - The Chinese journal of dental research
JID - 100892845
RN  - SOA12P041N (nitazoxanide)
RN  - 0 (beta Catenin)
RN  - 0 (Coloring Agents)
SB  - IM
MH  - *Adipogenesis
MH  - Osteogenesis/genetics
MH  - beta Catenin/genetics/metabolism/pharmacology
MH  - Cell Differentiation
MH  - *Mesenchymal Stem Cells
MH  - Coloring Agents
MH  - Cells, Cultured
OTO - NOTNLM
OT  - Wnt/beta-catenin pathway
OT  - adipogenesis
OT  - bone marrow mesenchymal stem cells
OT  - nitazoxanide
OT  - osteogenesis
EDAT- 2023/07/03 13:06
MHDA- 2023/07/04 06:42
CRDT- 2023/07/03 08:23
PHST- 2023/07/04 06:42 [medline]
PHST- 2023/07/03 13:06 [pubmed]
PHST- 2023/07/03 08:23 [entrez]
AID - 4128019 [pii]
AID - 10.3290/j.cjdr.b4128019 [doi]
PST - ppublish
SO  - Chin J Dent Res. 2023 Jun 23;26(2):69-75. doi: 10.3290/j.cjdr.b4128019.