PMID- 37395584
OWN - NLM
STAT- MEDLINE
DCOM- 20230705
LR  - 20230707
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 196
DP  - 2023 Jun 16
TI  - RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in 
      Human Osteosarcoma Cells.
LID - 10.3791/65545 [doi]
AB  - The important roles of long non-coding RNAs (lncRNAs) in cancer have been 
      studied, such as regulating the proliferation, epithelial-mesenchymal transition 
      (EMT), migration, infiltration, and autophagy of cancer cells. Localization 
      detection of lncRNAs in cells can provide insight into their functions. By 
      designing the lncRNA-specific antisense chain sequence followed by labeling with 
      fluorescent dyes, RNA fluorescence in situ hybridization (FISH) can be applied to 
      detect the cellular localization of lncRNAs. Together with the development of 
      microscopy, the RNA FISH techniques now even allow for visualization of the 
      poorly expressed lncRNAs. This method can not only detect the localization of 
      lncRNAs alone, but also detect the colocalization of other RNAs, DNA, or proteins 
      by using double-color or multicolor immunofluorescence. Here, we have included 
      the detailed experimental operation procedure and precautions of RNA FISH by 
      using lncRNA small nucleolar RNA host gene 6 (SNHG6) in human osteosarcoma cells 
      (143B) as an example, to provide a reference for researchers who want to perform 
      RNA FISH experiments, especially lncRNA FISH.
FAU - Chang, Junli
AU  - Chang J
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.
FAU - Ma, Xiaoping
AU  - Ma X
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.
FAU - Sun, Xingyuan
AU  - Sun X
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.
FAU - Zhou, Chujie
AU  - Zhou C
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.
FAU - Zhao, Peng
AU  - Zhao P
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.
FAU - Wang, Yongjun
AU  - Wang Y
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.
FAU - Yang, Yanping
AU  - Yang Y
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key 
      Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education; 
      yanpingyang@shutcm.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20230616
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Humans
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *MicroRNAs/genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - *Osteosarcoma/genetics
MH  - *Bone Neoplasms/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Cell Line, Tumor
EDAT- 2023/07/03 13:05
MHDA- 2023/07/05 06:42
CRDT- 2023/07/03 09:03
PHST- 2023/07/05 06:42 [medline]
PHST- 2023/07/03 13:05 [pubmed]
PHST- 2023/07/03 09:03 [entrez]
AID - 10.3791/65545 [doi]
PST - epublish
SO  - J Vis Exp. 2023 Jun 16;(196). doi: 10.3791/65545.