PMID- 37396151
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230704
IS  - 2225-4110 (Print)
IS  - 2225-4110 (Electronic)
IS  - 2225-4110 (Linking)
VI  - 13
IP  - 4
DP  - 2023 Jul
TI  - Chemoprevention of lotus leaf ethanolic extract through epigenetic activation of 
      the NRF2-mediated pathway in murine skin JB6 P+ cell neoplastic transformation.
PG  - 337-344
LID - 10.1016/j.jtcme.2023.02.002 [doi]
AB  - BACKGROUND AND AIM: Skin is one barrier protecting from environmental risk 
      factors that can make skin cells cancerous through DNA damage and oxidative 
      stress. The nuclear factor erythroid 2-related factor 2 (NRF2) pathway is an 
      anti-stress defense system that can be regulated by DNA methylation and histone 
      modification. Dietary phytochemicals have chemopreventive properties that can 
      inhibit or delay carcinogenesis. The lotus leaf is a traditional medicinal plant 
      containing many polyphenols whose extracts show many biological activities, 
      including antioxidant, anti-obesity, and anti-cancer. This study aim to 
      investigate the effect of lotus leaves on neoplastic transformation in murine 
      skin JB6 P+ cells. EXPERIMENTAL PROCEDURE: Lotus leaves were extracted with water 
      (LL-WE) and ethanol (LL-EE), and the LL-WE residues were further extracted with 
      ethanol (LL-WREE). JB6 P+ cells were treated with different extracts. The 
      chemoprotective effect would be evaluated by heme oxygenase 1 (HO-1), NAD(P)H 
      quinone oxidoreductase (NQO1), and UDP glucuronosyltransferase family 1 member A1 
      (UGT1A1) expression. RESULTS AND CONCLUSION: LL-EE contained higher total 
      phenolics and quercetin among extracts. In mouse skin JB6 P+ cells with 
      12-O-tetradecanoylphorbol-13-acetate treatment, LL-EE showed the greatest 
      potential to suppress skin carcinogenesis. LL-EE activated the NRF2 pathway by 
      upregulating antioxidant and detoxification enzymes upregulates antioxidant and 
      detoxification enzymes, including HO-1, NQO1, and UGT1A1, and downregulates DNA 
      methylation, which might be caused by lower DNA methyltransferase and histone 
      deacetylase levels. Therefore, our results show that LL-EE reduces the neoplastic 
      transformation of skin JB6 P+ cells, potentially by activating the NRF2 pathway 
      and regulating epigenetic DNA methylation and histone acetylation.
CI  - (c) 2023 Center for Food and Biomolecules, National Taiwan University. Production 
      and hosting by Elsevier Taiwan LLC.
FAU - Tung, Yen-Chen
AU  - Tung YC
AD  - Department of Food Science, National Ilan University, Yilan County, 260007, 
      Taiwan.
FAU - Sung, Ping-Hua
AU  - Sung PH
AD  - Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 
      City, 320314, Taiwan.
FAU - Chen, Pei-Chun
AU  - Chen PC
AD  - Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 
      City, 320314, Taiwan.
FAU - Wang, Hsiao Chi
AU  - Wang HC
AD  - Department of Oral Hygiene and Healthcare, Cardinal Tien Junior College of 
      Healthcare and Management, New Taipei City, 23143, Taiwan.
FAU - Lee, Jong Hun
AU  - Lee JH
AD  - Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120, 
      South Korea.
FAU - Su, Zheng-Yuan
AU  - Su ZY
AD  - Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 
      City, 320314, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20230225
PL  - Netherlands
TA  - J Tradit Complement Med
JT  - Journal of traditional and complementary medicine
JID - 101605474
PMC - PMC10310861
OTO - NOTNLM
OT  - Antioxidant
OT  - Cancer transformation
OT  - DNA methylation
OT  - Herbal medicine
OT  - Skin cell
COIS- The authors declare no conflicts of interest.
EDAT- 2023/07/03 13:05
MHDA- 2023/07/03 13:06
PMCR- 2023/02/25
CRDT- 2023/07/03 11:16
PHST- 2022/08/19 00:00 [received]
PHST- 2022/12/14 00:00 [revised]
PHST- 2023/02/20 00:00 [accepted]
PHST- 2023/07/03 13:06 [medline]
PHST- 2023/07/03 13:05 [pubmed]
PHST- 2023/07/03 11:16 [entrez]
PHST- 2023/02/25 00:00 [pmc-release]
AID - S2225-4110(23)00016-0 [pii]
AID - 10.1016/j.jtcme.2023.02.002 [doi]
PST - epublish
SO  - J Tradit Complement Med. 2023 Feb 25;13(4):337-344. doi: 
      10.1016/j.jtcme.2023.02.002. eCollection 2023 Jul.