PMID- 37408030
OWN - NLM
STAT- MEDLINE
DCOM- 20230707
LR  - 20230718
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 21
IP  - 1
DP  - 2023 Jul 5
TI  - Oral-microbiome-derived signatures enable non-invasive diagnosis of laryngeal 
      cancers.
PG  - 438
LID - 10.1186/s12967-023-04285-2 [doi]
LID - 438
AB  - BACKGROUND: Recent studies have uncovered that the microbiota in patients with 
      head and neck cancers is significantly altered and may drive cancer development. 
      However, there is limited data to explore the unique microbiota of laryngeal 
      squamous cell carcinoma (LSCC), and little is known regarding whether the oral 
      microbiota can be utilized as an early diagnostic biomarker. METHODS: Using 16S 
      rRNA gene sequencing, we characterized the microbiome of oral rinse and tissue 
      samples from 77 patients with LSCC and 76 control patients with vocal polyps, and 
      then performed bioinformatic analyses to identify taxonomic groups associated 
      with clinicopathologic features. RESULTS: Multiple bacterial genera exhibited 
      significant differences in relative abundance when stratifying by histologic and 
      tissue type. By exploiting the distinct microbial abundance and identifying the 
      tumor-associated microbiota taxa between patients of LSCC and vocal polyps, we 
      developed a predictive classifier by using rinse microbiota as key features for 
      the diagnosis of LSCC with 85.7% accuracy. CONCLUSION: This is 
      the first evidence of taxonomical features based on the oral rinse microbiome 
      that could diagnose LSCC. Our results revealed the oral rinse microbiome is an 
      understudied source of clinical variation and represents a potential non-evasive 
      biomarker of LSCC.
CI  - (c) 2023. The Author(s).
FAU - Yu, Shuting
AU  - Yu S
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 
      Shuaifuyuan, Dongcheng District, Beijing, China.
FAU - Chen, Junru
AU  - Chen J
AD  - Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, 
      Capital Medical University, Beijing, China.
FAU - Yan, Fangxu
AU  - Yan F
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 
      Shuaifuyuan, Dongcheng District, Beijing, China.
FAU - Fan, Yue
AU  - Fan Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 
      Shuaifuyuan, Dongcheng District, Beijing, China.
FAU - Xia, Xin
AU  - Xia X
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 
      Shuaifuyuan, Dongcheng District, Beijing, China.
FAU - Shan, Guangliang
AU  - Shan G
AD  - Department of Epidemiology and Statistics, Institute of Basic Medical Sciences, 
      Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union 
      Medical College, Beijing, China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Beijing Key Laboratory for Genetics of Birth Defects, Beijing Pediatric Research 
      Institute, MOE Key Laboratory of Major Diseases in Children, Rare Disease Center, 
      Beijing Children's Hospital, Capital Medical University, National Center for 
      Children's Health, Beijing, China. zhangpengdyx@163.com.
FAU - Chen, Xingming
AU  - Chen X
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 
      Shuaifuyuan, Dongcheng District, Beijing, China. xingming.chen@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230705
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - *Laryngeal Neoplasms/diagnosis/pathology
MH  - *Carcinoma, Squamous Cell/pathology
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Head and Neck Neoplasms
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - *Microbiota
MH  - Biomarkers
PMC - PMC10320982
OTO - NOTNLM
OT  - Biomarker
OT  - Diagnosis
OT  - Laryngeal cancer
OT  - Liquid biopsy
OT  - Microbiota
COIS- All authors declare no conflicts of interest.
EDAT- 2023/07/06 01:08
MHDA- 2023/07/07 06:42
PMCR- 2023/07/05
CRDT- 2023/07/05 23:45
PHST- 2023/04/03 00:00 [received]
PHST- 2023/06/17 00:00 [accepted]
PHST- 2023/07/07 06:42 [medline]
PHST- 2023/07/06 01:08 [pubmed]
PHST- 2023/07/05 23:45 [entrez]
PHST- 2023/07/05 00:00 [pmc-release]
AID - 10.1186/s12967-023-04285-2 [pii]
AID - 4285 [pii]
AID - 10.1186/s12967-023-04285-2 [doi]
PST - epublish
SO  - J Transl Med. 2023 Jul 5;21(1):438. doi: 10.1186/s12967-023-04285-2.