PMID- 374092
OWN - NLM
STAT- MEDLINE
DCOM- 19790725
LR  - 20061115
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 9
IP  - 1
DP  - 1979 Jan
TI  - Maturation of B lymphocytes. II. Sequential appearance of increasing IgM and IgD 
      in the adult bone marrow.
PG  - 39-44
AB  - The relationship between surface IgM (sIgM) and surface IgD (sIgD) was examined 
      on small lymphocytes in the adult murine bone marrow or prepubertal spleen. Cells 
      were sorted on the basis of different sIgM levels by a fluorescence-activated 
      cell sorter (FACS) and relabeled for sIgD or total sIg by a sandwich technique 
      using 125I-labeled protein A and radioautography. For detecting sIgD, an 
      anti-delta allotype reagent was used in congenic mice. Cells lacking sIgM in the 
      bone marrow or spleen were also found to be sIgD-; thus, sIgD appeared only in 
      the presence of sIgM. Weak sIgM-bearing cells in the bone marrow also had no sIgD 
      indicating that sIgD appeared only after the acquisition of a significant level 
      of sIgM. Subsequently, the incidence of sIgD+ cells increased in fractions 
      showing increasing sIgM levels indicating the acquisition of new sIgD by "sIgM 
      only" cells with increasing maturation levels in the bone marrow. In marrow 
      lymphoid cells expressing both Ig isotypes, sIgM and sIgD levels increased in 
      parallel, possibly with increasing maturation level. In the spleen, the incidence 
      of sIgD+ cells among various cell fractions showing different sIgM levels was 
      found constant. However, spleen cells bearing both receptors, showed a small 
      increase in the sIgD level with increasing sIgM level.
FAU - Lala, P K
AU  - Lala PK
FAU - Layton, J E
AU  - Layton JE
FAU - Nossal, G J
AU  - Nossal GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - *Aging
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Bone Marrow/*immunology
MH  - Cell Separation
MH  - Fluorescent Antibody Technique
MH  - Immunoglobulin D/*biosynthesis
MH  - Immunoglobulin M/*biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Antigen, B-Cell
MH  - Spleen/immunology
MH  - Time Factors
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 10.1002/eji.1830090110 [doi]
PST - ppublish
SO  - Eur J Immunol. 1979 Jan;9(1):39-44. doi: 10.1002/eji.1830090110.