PMID- 37409529
OWN - NLM
STAT- MEDLINE
DCOM- 20230831
LR  - 20240210
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 43
IP  - 9
DP  - 2023 Sep
TI  - Chronic Electronic Cigarette Use and Atherosclerosis Risk in Young People: A 
      Cross-Sectional Study-Brief Report.
PG  - 1713-1718
LID - 10.1161/ATVBAHA.123.319172 [doi]
AB  - BACKGROUND: Little is known whether electronic cigarettes (ECIG) increase 
      vulnerability to future atherosclerotic cardiovascular disease. We determined, 
      using an ex vivo mechanistic atherogenesis assay, whether proatherogenic changes 
      including monocyte transendothelial migration and monocyte-derived foam cell 
      formation are increased in people who use ECIGs. METHODS: In a cross-sectional 
      single-center study using plasma and peripheral blood mononuclear cells from 
      healthy participants who are nonsmokers or with exclusive use of ECIGs or tobacco 
      cigarettes (TCIGs), autologous peripheral blood mononuclear cells with patient 
      plasma and pooled peripheral blood mononuclear cells from healthy nonsmokers with 
      patient plasma were utilized to dissect patient-specific ex vivo proatherogenic 
      circulating factors present in plasma and cellular factors present in monocytes. 
      Our main outcomes were monocyte transendothelial migration (% of blood monocyte 
      cells that undergo transendothelial migration through a collagen gel) and 
      monocyte-derived foam cell formation as determined by flow cytometry and the 
      median fluorescence intensity of the lipid-staining fluorochrome BODIPY in 
      monocytes of participants in the setting of an ex vivo model of atherogenesis. 
      RESULTS: Study participants (N=60) had median age of 24.0 years (interquartile 
      range [IQR], 22.0-25.0 years), and 31 were females. Monocyte transendothelial 
      migration was increased in people who exclusively used TCIGs (n=18; median [IQR], 
      2.30 [ 1.29-2.82]; P<0.001) and in people who exclusively used ECIGs (n=21; 
      median [IQR], 1.42 [ 0.96-1.91]; P<0.01) compared with nonsmoking controls (n=21; 
      median [IQR], 1.05 [0.66-1.24]). Monocyte-derived foam cell formation was 
      increased in people who exclusively used TCIGs (median [IQR], 2.01 [ 1.59-2.49]; 
      P<0.001) and in people who exclusively used ECIGs (median [IQR], 1.54 [ 
      1.10-1.86]; P<0.001) compared with nonsmoker controls (median [IQR], 0.97 
      [0.86-1.22]). Both monocyte transendothelial migration and monocyte-derived foam 
      cell formation were higher in TCIG smokers compared with ECIG users and in ECIG 
      users who were former smokers versus ECIG users who were never smokers (P<0.05 
      for all comparisons). CONCLUSIONS: The finding of alterations in proatherogenic 
      properties of blood monocytes and plasma in TCIG smokers compared with nonsmokers 
      validates this assay as a strong ex vivo mechanistic tool with which to measure 
      proatherogenic changes in people who use ECIGs. Similar yet significantly less 
      severe alterations in proatherogenic properties of monocytes and plasma were 
      detected in the blood from ECIG users. Future studies are necessary to determine 
      whether these findings are attributable to a residual effect of prior smoking or 
      are a direct effect of current ECIG use.
FAU - Kelesidis, Theodoros
AU  - Kelesidis T
AUID- ORCID: 0000-0002-8463-3811
AD  - Division of Infectious Disease, Department of Medicine (T.K., M.S., E.S.), David 
      Geffen School of Medicine at UCLA.
FAU - Sharma, Madhav
AU  - Sharma M
AD  - Division of Infectious Disease, Department of Medicine (T.K., M.S., E.S.), David 
      Geffen School of Medicine at UCLA.
FAU - Sharma, Eashan
AU  - Sharma E
AD  - Division of Infectious Disease, Department of Medicine (T.K., M.S., E.S.), David 
      Geffen School of Medicine at UCLA.
FAU - Ruedisueli, Isabelle
AU  - Ruedisueli I
AUID- ORCID: 0000-0002-2352-9738
AD  - Division of Cardiology, Department of Medicine (I.R., E.T., H.R.M.), David Geffen 
      School of Medicine at UCLA.
FAU - Tran, Elizabeth
AU  - Tran E
AD  - Division of Cardiology, Department of Medicine (I.R., E.T., H.R.M.), David Geffen 
      School of Medicine at UCLA.
FAU - Middlekauff, Holly R
AU  - Middlekauff HR
AUID- ORCID: 0000-0001-7848-8585
AD  - Division of Cardiology, Department of Medicine (I.R., E.T., H.R.M.), David Geffen 
      School of Medicine at UCLA.
LA  - eng
GR  - P30 AI028697/AI/NIAID NIH HHS/United States
GR  - R01 AG059502/AG/NIA NIH HHS/United States
GR  - R43 AG059501/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230706
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
SB  - IM
MH  - Adult
MH  - Female
MH  - Humans
MH  - Male
MH  - Young Adult
MH  - *Atherosclerosis/etiology
MH  - Cross-Sectional Studies
MH  - *Electronic Nicotine Delivery Systems
MH  - Leukocytes, Mononuclear
MH  - *Vaping/adverse effects
PMC - PMC10527452
MID - NIHMS1911796
OTO - NOTNLM
OT  - atherosclerosis
OT  - inflammation
OT  - monocytes
OT  - risk
OT  - smokers
COIS- Disclosures None.
EDAT- 2023/07/06 06:42
MHDA- 2023/08/25 06:42
PMCR- 2024/09/01
CRDT- 2023/07/06 05:36
PHST- 2024/09/01 00:00 [pmc-release]
PHST- 2023/08/25 06:42 [medline]
PHST- 2023/07/06 06:42 [pubmed]
PHST- 2023/07/06 05:36 [entrez]
AID - 10.1161/ATVBAHA.123.319172 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2023 Sep;43(9):1713-1718. doi: 
      10.1161/ATVBAHA.123.319172. Epub 2023 Jul 6.