PMID- 37414380
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231106
IS  - 1521-7035 (Electronic)
IS  - 1521-6616 (Linking)
VI  - 253
DP  - 2023 Aug
TI  - AXL(+)SIGLEC6(+) dendritic cells in cerebrospinal fluid and brain tissues of 
      patients with autoimmune inflammatory demyelinating disease of CNS.
PG  - 109686
LID - S1521-6616(23)00449-7 [pii]
LID - 10.1016/j.clim.2023.109686 [doi]
AB  - Inflammatory demyelinating disease of the CNS (IDD) is a heterogeneous group of 
      autoimmune diseases, and multiple sclerosis is the most common type. Dendritic 
      cells (DCs), major antigen-presenting cells, have been proposed to play a central 
      role in the pathogenesis of IDD. The AXL(+)SIGLEC6(+) DC (ASDC) has been only 
      recently identified in humans and has a high capability of T cell activation. 
      Nevertheless, its contribution to CNS autoimmunity remains still obscure. Here, 
      we aimed to identify the ASDC in diverse sample types from IDD patients and 
      experimental autoimmune encephalomyelitis (EAE). A detailed analysis of DC 
      subpopulations using single-cell transcriptomics for the paired cerebrospinal 
      fluid (CSF) and blood samples of IDD patients (total n = 9) revealed that three 
      subtypes of DCs (ASDCs, ACY3(+) DCs, and LAMP3(+) DCs) were overrepresented in 
      CSF compared with their paired blood. Among these DCs, ASDCs were also more 
      abundant in CSF of IDD patients than in controls, manifesting poly-adhesional and 
      stimulatory characteristics. In the brain biopsied tissues of IDD patients, 
      obtained at the acute attack of disease, ASDC were also frequently found in close 
      contact with T cells. Lastly, the frequency of ASDC was found to be temporally 
      more abundant in acute attack of disease both in CSF samples of IDD patients and 
      in tissues of EAE, an animal model for CNS autoimmunity. Our analysis suggests 
      that the ASDC might be involved in the pathogenesis of CNS autoimmunity.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Kang, Junho
AU  - Kang J
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology, Daejeon, Republic of Korea.
FAU - Kim, Moonhang
AU  - Kim M
AD  - Biomedical Research Institute, Seoul National University Hospital, Seoul, 
      Republic of Korea.
FAU - Yoon, Da-Young
AU  - Yoon DY
AD  - Department of Biomedical Sciences, Seoul National University College of Medicine, 
      Seoul, Republic of Korea.
FAU - Kim, Woo-Seok
AU  - Kim WS
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology, Daejeon, Republic of Korea.
FAU - Choi, Seok-Jin
AU  - Choi SJ
AD  - Department of Neurology, Seoul National University Hospital, Seoul National 
      University of Medicine, Seoul, Republic of Korea.
FAU - Kwon, Young-Nam
AU  - Kwon YN
AD  - Department of Neurology, Seoul National University Hospital, Seoul National 
      University of Medicine, Seoul, Republic of Korea.
FAU - Kim, Won-Seok
AU  - Kim WS
AD  - Department of Neurology, Seoul National University Hospital, Seoul National 
      University of Medicine, Seoul, Republic of Korea.
FAU - Park, Sung-Hye
AU  - Park SH
AD  - Department of Pathology, Seoul National University Hospital, Seoul National 
      University of Medicine, Seoul, Republic of Korea.
FAU - Sung, Jung-Joon
AU  - Sung JJ
AD  - Department of Neurology, Seoul National University Hospital, Seoul National 
      University of Medicine, Seoul, Republic of Korea.
FAU - Park, Myungsun
AU  - Park M
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology, Daejeon, Republic of Korea.
FAU - Lee, Jung Seok
AU  - Lee JS
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology, Daejeon, Republic of Korea.
FAU - Park, Jong-Eun
AU  - Park JE
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology, Daejeon, Republic of Korea. Electronic address: 
      jp24@kaist.ac.kr.
FAU - Kim, Sung-Min
AU  - Kim SM
AD  - Department of Neurology, Seoul National University Hospital, Seoul National 
      University of Medicine, Seoul, Republic of Korea. Electronic address: 
      sueh916@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230704
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (SIGLEC6 protein, human)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Antigens, CD)
RN  - 0 (Lectins)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Encephalomyelitis, Autoimmune, Experimental
MH  - *Multiple Sclerosis
MH  - T-Lymphocytes
MH  - Brain/pathology
MH  - Dendritic Cells
MH  - Antigens, Differentiation, Myelomonocytic
MH  - Antigens, CD
MH  - Lectins
OTO - NOTNLM
OT  - AXL(+)SIGLEC6(+) dendritic cell
OT  - Inflammatory demyelinating disease
OT  - Single-cell RNA sequencing
COIS- Declaration of Competing Interest S.M.K has lectured, consulted, and received 
      honoraria from Bayer Schering Pharma, Genzyme, Merck Serono, and UCB; received a 
      grant from the National Research Foundation of Korea and the Korea Health 
      Industry Development Institute Research; is an Associate Editor of the Journal of 
      Clinical Neurology. S.M.K and Seoul National University Hospital have transferred 
      the technology of flow cytometric autoantibody assay to EONE Laboratory, Korea. 
      The remaining authors declare no competing interests.
EDAT- 2023/07/07 01:05
MHDA- 2023/10/23 00:44
CRDT- 2023/07/06 19:29
PHST- 2023/03/19 00:00 [received]
PHST- 2023/05/26 00:00 [revised]
PHST- 2023/06/12 00:00 [accepted]
PHST- 2023/10/23 00:44 [medline]
PHST- 2023/07/07 01:05 [pubmed]
PHST- 2023/07/06 19:29 [entrez]
AID - S1521-6616(23)00449-7 [pii]
AID - 10.1016/j.clim.2023.109686 [doi]
PST - ppublish
SO  - Clin Immunol. 2023 Aug;253:109686. doi: 10.1016/j.clim.2023.109686. Epub 2023 Jul 
      4.