PMID- 37415425
OWN - NLM
STAT- MEDLINE
DCOM- 20231113
LR  - 20231113
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 61
IP  - 9
DP  - 2023 Nov 5
TI  - GC Analysis of Metformin, Ranitidine and Famotidine from Pharmaceuticals and 
      Human Serum.
PG  - 807-813
LID - 10.1093/chromsci/bmad047 [doi]
AB  - A method has been designed based on gas chromatography with flame ionization 
      detection (FID) for the separation and analyses of ranitidine, famotidine and 
      metformin after pre-column derivatization with trifluoroacetylacetone and ethyl 
      chloroformate. DB-1 (30 m x 0.32 mm id) column with film thickness 0.25 mum was 
      used for the separation at an initial temperature of column was 100 degrees C for 2 min, 
      and ramping at 20 degrees C/min up to 250 degrees C, with a hold time of 3 min. The rate of 
      nitrogen flow was 2.5 mL/min and FID was used for detection. Complete separation 
      was obtained between all the three drugs including excess of derivatization 
      reagents. Linear calibration curves and detection limits were obtained in the 
      ranges 0.1-30 mug/mL and 0.011-0.015 mug/mL. The procedure was repeatable in terms 
      of peak heights/peak areas and retention time (n = 5) for derivatization, 
      quantitation and separation with relative standard deviations (RSDs) within 
      2.0-3.0%. The approach was examined for the analyses of drug products and serum 
      after the intake of the drugs by healthy volunteers, and recoveries were obtained 
      within 95-98% with RSDs 2.4-3.1%.
CI  - (c) The Author(s) 2023. Published by Oxford University Press. All rights reserved. 
      For permissions, please email: journals.permissions@oup.com.
FAU - Zounr, Rizwan A
AU  - Zounr RA
AD  - Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, 
      Jamshoro 76080, Sindh, Pakistan.
FAU - Khuhawar, Muhammad Y
AU  - Khuhawar MY
AD  - Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, 
      Jamshoro 76080, Sindh, Pakistan.
FAU - Khuhawar, Taj M J
AU  - Khuhawar TMJ
AD  - Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, 
      Jamshoro 76080, Sindh, Pakistan.
FAU - Lanjwani, Muhammad F
AU  - Lanjwani MF
AUID- ORCID: 0000-0001-8794-7296
AD  - Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro 76080, Sindh, 
      Pakistan.
FAU - Khuhawar, Muzamil Y
AU  - Khuhawar MY
AD  - Abbott Pharmaceutical Company in Korangi, Landhi Karachi 75106, Sindh, Pakistan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 5QZO15J2Z8 (Famotidine)
RN  - 884KT10YB7 (Ranitidine)
RN  - 9100L32L2N (Metformin)
RN  - 0 (Pharmaceutical Preparations)
SB  - IM
MH  - Humans
MH  - *Famotidine
MH  - Ranitidine
MH  - *Metformin
MH  - Chromatography, Gas/methods
MH  - Pharmaceutical Preparations
EDAT- 2023/07/07 06:42
MHDA- 2023/11/13 06:42
CRDT- 2023/07/07 02:34
PHST- 2022/02/20 00:00 [received]
PHST- 2023/05/24 00:00 [revised]
PHST- 2023/06/18 00:00 [accepted]
PHST- 2023/11/13 06:42 [medline]
PHST- 2023/07/07 06:42 [pubmed]
PHST- 2023/07/07 02:34 [entrez]
AID - 7220449 [pii]
AID - 10.1093/chromsci/bmad047 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2023 Nov 5;61(9):807-813. doi: 10.1093/chromsci/bmad047.