PMID- 37415750
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 2229-5070 (Print)
IS  - 2229-7758 (Electronic)
IS  - 2229-5070 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Jan-Jun
TI  - In silico study to predict promiscuous peptides for immunodiagnosis of cystic 
      echinococcosis.
PG  - 54-62
LID - 10.4103/tp.tp_70_22 [doi]
AB  - BACKGROUND: Cystic echinococcosis (CE), caused by Echinococcus granulosus, is a 
      major zoonotic disease that causes significant human morbidity and mortality. 
      This cosmopolitan disease is difficult to diagnose, treat, and control. So far, 
      crude extracts of hydatid cyst fluid containing antigen B or antigen 5 have been 
      used as the primary antigenic source for its immunodiagnosis. The main issue is 
      that it reacts with sera from people infected with other helminths. There is 
      currently no standard, specific, or sensitive test for disease diagnosis, and no 
      human vaccine has been reported. AIMS AND OBJECTIVES: Considering the need for 
      efficient immunization and/or immunodiagnosis, six E. granulosus antigens, 
      antigen 5, antigen B, heat shock proteins such as Hsp-8 and Hsp-90, 
      phosphoenolpyruvate carboxykinase, and tetraspanin-1, were chosen. MATERIALS AND 
      METHODS: Using various in silico tools, T cell and B cell epitopes (promiscuous 
      peptides) were predicted by targeting antigen 5, antigen B, heat shock proteins 
      such as Hsp-8 and Hsp-90, phosphoenolpyruvate carboxykinase, and tetraspanin-1. 
      RESULTS: There are twelve promiscuous peptides with overlapping human leukocyte 
      antigen (HLA) class-I, class-II, and conformational B cell epitopes. Such 
      immunodominant peptides could be useful as subunit vaccines. Furthermore, six 
      peptides specific for E. granulosus were also discovered, which may prove to be 
      important markers in the diagnosis of CE, potentially preventing misdiagnosis and 
      mismanagement. CONCLUSION: These epitopes may be the most important vaccine 
      targets in E. granulosus because they have the most promiscuous peptides and B 
      cell epitopes, as well as the highest affinity for different alleles, as 
      determined by docking scores. However, additional research using in vitro and in 
      vivo models is undertaken.
CI  - Copyright: (c) 2023 Tropical Parasitology.
FAU - Chauhan, Varun
AU  - Chauhan V
AD  - Department of Microbiology, Faculty of Applied Sciences and Biotechnology, 
      Shoolini University, Solan, India.
AD  - Department of Dietetic and Nutrition Technology, CSIR-Institute of Himalayan 
      Bioresource Technology, Palampur, Himachal Pradesh, India.
FAU - Khan, Azhar
AU  - Khan A
AD  - Department of Microbiology, Faculty of Applied Sciences and Biotechnology, 
      Shoolini University, Solan, India.
FAU - Farooq, Umar
AU  - Farooq U
AD  - Department of Microbiology, Faculty of Applied Sciences and Biotechnology, 
      Shoolini University, Solan, India.
AD  - Department of Basic Oral Medicine and Allied Dental Science, College of 
      Dentistry, Taif University, Taif, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20230519
PL  - India
TA  - Trop Parasitol
JT  - Tropical parasitology
JID - 101580198
PMC - PMC10321577
OTO - NOTNLM
OT  - B and T cell epitopes
OT  - cystic echinococcosis
OT  - human leukocyte antigen
OT  - immunodiagnostic
OT  - promiscuous
OT  - subunit vaccine
COIS- There are no conflicts of interest.
EDAT- 2023/07/07 06:42
MHDA- 2023/07/07 06:43
PMCR- 2023/01/01
CRDT- 2023/07/07 03:57
PHST- 2022/10/30 00:00 [received]
PHST- 2023/02/26 00:00 [revised]
PHST- 2023/03/15 00:00 [accepted]
PHST- 2023/07/07 06:43 [medline]
PHST- 2023/07/07 06:42 [pubmed]
PHST- 2023/07/07 03:57 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - TP-13-54 [pii]
AID - 10.4103/tp.tp_70_22 [doi]
PST - ppublish
SO  - Trop Parasitol. 2023 Jan-Jun;13(1):54-62. doi: 10.4103/tp.tp_70_22. Epub 2023 May 
      19.