PMID- 37417004
OWN - NLM
STAT- MEDLINE
DCOM- 20231030
LR  - 20231103
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Print)
IS  - 1017-7825 (Linking)
VI  - 33
IP  - 10
DP  - 2023 Oct 28
TI  - Crystal Structures of 6-Phosphogluconate Dehydrogenase from Corynebacterium 
      glutamicum.
PG  - 1361-1369
LID - 10.4014/jmb.2305.05002 [doi]
AB  - Corynebacterium glutamicum (C. glutamicum) has been considered a very important 
      and meaningful industrial microorganism for the production of amino acids 
      worldwide. To produce amino acids, cells require nicotinamide adenine 
      dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose 
      phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate 
      dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 
      6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In 
      this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from C. 
      glutamicum ATCC 13032 (Cg6PGD) and reported our biological research based on this 
      structure. We identified the substrate binding site and co-factor binding site of 
      Cg6PGD, which are crucial for understanding this enzyme. Based on the findings of 
      our research, Cg6PGD is expected to be used as a NADPH resource in the food 
      industry and as a drug target in the pharmaceutical industry.
FAU - Yu, Hyeonjeong
AU  - Yu H
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook 
      National University, Daegu 41566, Republic of Korea.
FAU - Hong, Jiyeon
AU  - Hong J
AD  - KNU Institute for Microorganisms, Kyungpook National University, Daegu 41566, 
      Republic of Korea.
FAU - Seok, Jihye
AU  - Seok J
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook 
      National University, Daegu 41566, Republic of Korea.
FAU - Seu, Young-Bae
AU  - Seu YB
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook 
      National University, Daegu 41566, Republic of Korea.
AD  - KNU Institute for Microorganisms, Kyungpook National University, Daegu 41566, 
      Republic of Korea.
FAU - Kim, Il-Kwon
AU  - Kim IK
AD  - KNU Institute for Microorganisms, Kyungpook National University, Daegu 41566, 
      Republic of Korea.
FAU - Kim, Kyung-Jin
AU  - Kim KJ
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook 
      National University, Daegu 41566, Republic of Korea.
AD  - KNU Institute for Microorganisms, Kyungpook National University, Daegu 41566, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20230627
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 53-59-8 (NADP)
RN  - EC 1.1.1.43 (Phosphogluconate Dehydrogenase)
RN  - 0 (Amino Acids)
SB  - IM
MH  - *Corynebacterium glutamicum/metabolism
MH  - NADP/metabolism
MH  - Phosphogluconate Dehydrogenase/metabolism
MH  - Pentose Phosphate Pathway
MH  - Amino Acids/metabolism
PMC - PMC10619557
OTO - NOTNLM
OT  - 6-Phosphogluconate dehydrogenase
OT  - 6-phosphogluconate
OT  - Corynebacterium glutamicum
OT  - crystal structure
OT  - nicotinamide adenine dinucleotide phosphate
EDAT- 2023/07/07 06:42
MHDA- 2023/10/30 06:46
PMCR- 2023/06/27
CRDT- 2023/07/07 04:33
PHST- 2023/05/04 00:00 [received]
PHST- 2023/06/11 00:00 [revised]
PHST- 2023/06/16 00:00 [accepted]
PHST- 2023/10/30 06:46 [medline]
PHST- 2023/07/07 06:42 [pubmed]
PHST- 2023/07/07 04:33 [entrez]
PHST- 2023/06/27 00:00 [pmc-release]
AID - jmb.2305.05002 [pii]
AID - jmb-33-10-1361 [pii]
AID - 10.4014/jmb.2305.05002 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2023 Oct 28;33(10):1361-1369. doi: 
      10.4014/jmb.2305.05002. Epub 2023 Jun 27.