PMID- 37418079
OWN - NLM
STAT- MEDLINE
DCOM- 20230829
LR  - 20230831
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 50
IP  - 9
DP  - 2023 Sep
TI  - The role of non-classical and chain-related human leukocyte antigen polymorphisms 
      in laryngeal squamous cell carcinoma.
PG  - 7245-7252
LID - 10.1007/s11033-023-08629-5 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the major pathological 
      subtype of laryngeal cancer. It has been shown that alterations of the expression 
      of non-classical human leukocyte antigens (HLA) and the chain-related MIC 
      molecules by malignant cells can lead to escape from the immune system control 
      and certain allele variants may participate in immune editing and therefore be 
      associated with modulation of cancer risk. The aim of the present study was to 
      investigate the role of non-classical HLA class Ib and chain-related MIC 
      polymorphisms, determined at the allelic level by next-generation sequencing 
      (NGS), in patients from the Bulgarian population, diagnosed with LSCC. MATERIALS 
      AND METHODS: In the present study DNA samples from 48 patients with LSCC were 
      used. Data was compared to 63 healthy controls analysed in previous studies. HLA 
      genotyping was performed by using the AlloSeq Tx17 early pooling protocol and the 
      library preparation AlloSeq Tx17 kit (CareDx). Sequencing was performed on 
      MiniSeq sequencing platform (Illumina) and HLA genotypes were assigned with the 
      AlloSeq Assign analysis software v1.0.3 (CareDx) and the IPD-IMGT/HLA database 
      3.45.1.2. RESULTS: The HLA disease association tests revealed a statistically 
      significant predisposing association of HLA-F*01:01:02 (Pc = 0.0103, 
      OR = 24.0194) with LSCC, while HLA-F*01:01:01 (Pc = 8.21e-04, OR = 0.0485) has a 
      possible protective association. Additionally we observed several haplotypes with 
      statistically significant protective and predisposing associations. The strongest 
      association was observed for F*01:01:01-H*01:01:01 (P = 0.0054, haplotype 
      score=-2.7801). CONCLUSION: Our preliminary study suggests the involvement of HLA 
      class Ib in cancer development and the possible role of the shown alleles as 
      biomarkers of LSCC.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Ormandjieva, Anastasia
AU  - Ormandjieva A
AUID- ORCID: 0000-0002-3687-9390
AD  - Department of Clinical Immunology, Medical Faculty, Medical University, Sofia, 
      Bulgaria. anastasia.ormandjieva@gmail.com.
FAU - Yordanov, Stanislav
AU  - Yordanov S
AD  - University Hospital "St. Anna", Sofia, Bulgaria.
FAU - Stoyanov, Hristo
AU  - Stoyanov H
AD  - Department of Dental, Oral and Maxillofacial surgery, FDM, Medical University - 
      Sofia, Sofia, Bulgaria.
FAU - Deliverska, Elitsa
AU  - Deliverska E
AUID- ORCID: 0000-0002-2356-9932
AD  - Department of Dental, Oral and Maxillofacial surgery, FDM, Medical University - 
      Sofia, Sofia, Bulgaria.
FAU - Shivarov, Velizar
AU  - Shivarov V
AUID- ORCID: 0000-0001-5362-7999
AD  - Department of Experimental Research, Medical University Pleven, Pleven, Bulgaria.
FAU - Ivanova, Milena
AU  - Ivanova M
AUID- ORCID: 0000-0003-4128-9915
AD  - Department of Clinical Immunology, Medical Faculty, Medical University, Sofia, 
      Bulgaria.
AD  - Clinic of Clinical Immunology with Stem Cell Bank, University Hospital 
      ''Alexandrovska'', Sofia, Bulgaria.
LA  - eng
GR  - C-126/04.06.2021/Medical University Sofia/
GR  - KP-06-H23/4/Bulgarian National Science Fund/
PT  - Journal Article
DEP - 20230707
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Histocompatibility Antigens Class II/genetics
MH  - Polymorphism, Genetic/genetics
MH  - HLA Antigens/genetics
MH  - Histocompatibility Antigens Class I/genetics
MH  - Haplotypes/genetics
MH  - *Head and Neck Neoplasms/genetics
MH  - Alleles
MH  - Gene Frequency/genetics
MH  - *Laryngeal Neoplasms/genetics/metabolism
OTO - NOTNLM
OT  - Association studies
OT  - LSCC
OT  - Larynx carcinoma
OT  - MIC molecules
OT  - Non-classical HLA
EDAT- 2023/07/07 13:04
MHDA- 2023/08/29 12:44
CRDT- 2023/07/07 11:11
PHST- 2023/03/27 00:00 [received]
PHST- 2023/06/23 00:00 [accepted]
PHST- 2023/08/29 12:44 [medline]
PHST- 2023/07/07 13:04 [pubmed]
PHST- 2023/07/07 11:11 [entrez]
AID - 10.1007/s11033-023-08629-5 [pii]
AID - 10.1007/s11033-023-08629-5 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2023 Sep;50(9):7245-7252. doi: 10.1007/s11033-023-08629-5. Epub 
      2023 Jul 7.