PMID- 37419489
OWN - NLM
STAT- MEDLINE
DCOM- 20231009
LR  - 20231018
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Linking)
VI  - 41
IP  - 10
DP  - 2023 Oct 8
TI  - hucMSCs Treatment Ameliorated Pulmonary Fibrosis via Downregulating the 
      circFOXP1-HuR-EZH2/STAT1/FOXK1 Autophagic Axis.
PG  - 928-943
LID - 10.1093/stmcls/sxad053 [doi]
AB  - This study was performed to determine the effect of human umbilical cord 
      mesenchymal stem cells (hucMSCs) treatment on pulmonary fibrosis and investigate 
      the circFOXP1-mediated autophagic mechanism of hucMSCs treatment. Pulmonary 
      fibrosis models were established by spraying bleomycin in mice and TGF-beta1 
      treatment of MRC-5 cells. Results showed that hucMSCs were retained in lung and 
      hucMSCs treatment alleviated pulmonary fibrosis. Morphological staining indicated 
      that hucMSCs-treated mice had thinner alveolar walls, effectively improved 
      alveolar structure, significantly reduced alveolar inflammation, and decreased 
      collagen deposition than control mice. Fibrotic proteins, including vimentin, 
      alpha-SMA, collagens I and III, and the differentiation-related protein S100 
      calcium-binding protein A4 was reduced considerably in the hucMSCs-treated group. 
      The mechanistic study revealed that the inhibition of hucMSCs treatment on 
      pulmonary fibrogenesis depended on downregulating circFOXP1, in which hucMSCs 
      treatment promoted circFOXP1-mediated autophagy process via blocking the nuclear 
      human antigen R (HuR) translocation and promoting the HuR degradation, leading to 
      a marked decrease in autophagy negative regulators EZH2, STAT1, and FOXK1. In 
      conclusion, hucMSCs treatment significantly improved pulmonary fibrosis by 
      downregulating the circFOXP1-HuR-EZH2/STAT1/FOXK1 autophagic axis. hucMSCs can 
      act as an effective treatment for pulmonary fibrosis.
CI  - (c) The Author(s) 2023. Published by Oxford University Press. All rights reserved. 
      For permissions, please email: journals.permissions@oup.com.
FAU - Li, Ruiqiong
AU  - Li R
AD  - Department of Clinical Nursing, Binzhou Medical University Hospital, Binzhou 
      Medical University, Binzhou, People's Republic of China.
FAU - Zhang, Haitong
AU  - Zhang H
AD  - Department of Respiratory and Critical Care Medicine, Binzhou Medical University 
      Hospital, Binzhou Medical University, Binzhou, People's Republic of China.
FAU - Zhang, Jinjin
AU  - Zhang J
AD  - Medical Research Center, Binzhou Medical University, Yantai, People's Republic of 
      China.
FAU - Ji, Yunxia
AU  - Ji Y
AD  - Department of Respiratory and Critical Care Medicine, Binzhou Medical University 
      Hospital, Binzhou Medical University, Binzhou, People's Republic of China.
FAU - Liu, Wenbo
AU  - Liu W
AD  - Medical Research Center, Binzhou Medical University, Yantai, People's Republic of 
      China.
FAU - Liu, Weili
AU  - Liu W
AD  - Department of Respiratory and Critical Care Medicine, Binzhou Medical University 
      Hospital, Binzhou Medical University, Binzhou, People's Republic of China.
FAU - Wang, Meirong
AU  - Wang M
AD  - Medical Research Center, Binzhou Medical University, Yantai, People's Republic of 
      China.
FAU - Lv, Changjun
AU  - Lv C
AD  - Department of Respiratory and Critical Care Medicine, Binzhou Medical University 
      Hospital, Binzhou Medical University, Binzhou, People's Republic of China.
FAU - Song, Xiaodong
AU  - Song X
AD  - Department of Cellular and Genetic Medicine, School of Pharmaceutical Sciences, 
      Binzhou Medical University, Yantai, People's Republic of China.
FAU - Li, Hongbo
AU  - Li H
AD  - Department of Respiratory and Critical Care Medicine, Binzhou Medical University 
      Hospital, Binzhou Medical University, Binzhou, People's Republic of China.
FAU - Li, Minge
AU  - Li M
AUID- ORCID: 0000-0002-8995-3443
AD  - Department of Clinical Nursing, Binzhou Medical University Hospital, Binzhou 
      Medical University, Binzhou, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
RN  - 0 (STAT1 protein, human)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (FOXK1 protein, human)
RN  - 0 (Forkhead Transcription Factors)
SB  - IM
MH  - Mice
MH  - Humans
MH  - Animals
MH  - *Pulmonary Fibrosis/therapy
MH  - Fibrosis
MH  - Lung/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Autophagy
MH  - Umbilical Cord
MH  - Enhancer of Zeste Homolog 2 Protein/genetics/metabolism
MH  - STAT1 Transcription Factor
MH  - Forkhead Transcription Factors/metabolism
OTO - NOTNLM
OT  - HuR
OT  - autophagy
OT  - circRNA
OT  - human umbilical cord mesenchymal stem cells
OT  - pulmonary fibrosis
EDAT- 2023/07/08 10:41
MHDA- 2023/10/09 06:42
CRDT- 2023/07/07 19:43
PHST- 2022/12/06 00:00 [received]
PHST- 2023/06/13 00:00 [accepted]
PHST- 2023/10/09 06:42 [medline]
PHST- 2023/07/08 10:41 [pubmed]
PHST- 2023/07/07 19:43 [entrez]
AID - 7221330 [pii]
AID - 10.1093/stmcls/sxad053 [doi]
PST - ppublish
SO  - Stem Cells. 2023 Oct 8;41(10):928-943. doi: 10.1093/stmcls/sxad053.