PMID- 37423368
OWN - NLM
STAT- MEDLINE
DCOM- 20230912
LR  - 20230920
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 234
DP  - 2023 Oct 1
TI  - Insights in MICP dynamics in urease-positive Staphylococcus sp. H6 and 
      Sporosarcina pasteurii bacterium.
PG  - 116588
LID - S0013-9351(23)01392-0 [pii]
LID - 10.1016/j.envres.2023.116588 [doi]
AB  - Microbially induced calcite precipitation (MICP) is an efficient and eco-friendly 
      technique that has attracted significant interest for resolving various problems 
      in the soil (erosion, improving structural integrity and water retention, etc.), 
      remediation of heavy metals, production of self-healing concrete or restoration 
      of different concrete structures. The success of most common MICP methods depends 
      on microorganisms degrading urea which leads to the formation of CaCO(3) 
      crystals. While Sporosarcina pasteurii is a well-known microorganism for MICP, 
      other soil abundant microorganisms, such as Staphylococcus bacteria have not been 
      thoroughly studied for its efficiency in bioconsolidation though MICP is a very 
      important proccess which can ensure soil quality and health. This study aimed to 
      analyze MICP process at the surface level in Sporosarcina pasteurii and a newly 
      screened Staphylococcus sp. H6 bacterium as well as show the possibility of this 
      new microorganism to perform MICP. It was observed that Staphylococcus sp. H6 
      culture precipitated 157.35 +/- 3.3 mM of Ca(2+) ions from 200 mM, compared to 
      176 +/- 4.8 mM precipitated by S. pasteurii. The bioconsolidation of sand particles 
      was confirmed by Raman spectroscopy and XRD analysis, which indicated the 
      formation of CaCO(3) crystals for both Staphylococcus sp. H6 and S. pasteurii 
      cells. The water-flow test suggested a significant reduction in water 
      permeability in bioconsolidated sand samples for both Staphylococcus sp. H6 and 
      S. pasteurii. Notably, this study provides the first evidence that CaCO(3) 
      precipitation occurs on the surface of Staphylococcus and S. pasteurii cells 
      within the initial 15-30 min after exposure to the biocementation solution. 
      Furthermore, Atomic force microscopy (AFM) indicated rapid changes in cell 
      roughness, with bacterial cells becoming completely coated with CaCO(3) crystals 
      after 90 min incubation with a biocementation solution. To our knowledge, this is 
      the first time where atomic force microscopy was used to visualize the dynamic of 
      MICP on cell surface.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Vaskevicius, Laurynas
AU  - Vaskevicius L
AD  - Department of Microbiology and Biotechnology, Institute of Biosciences, Life 
      Sciences Center, Vilnius University, Sauletekis Av. 7, LT-10257, Vilnius, 
      Lithuania.
FAU - Malunavicius, Vilius
AU  - Malunavicius V
AD  - Department of Microbiology and Biotechnology, Institute of Biosciences, Life 
      Sciences Center, Vilnius University, Sauletekis Av. 7, LT-10257, Vilnius, 
      Lithuania.
FAU - Jankunec, Marija
AU  - Jankunec M
AD  - Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekis 
      Av. 7, LT-10257, Vilnius, Lithuania.
FAU - Lastauskiene, Egle
AU  - Lastauskiene E
AD  - Department of Microbiology and Biotechnology, Institute of Biosciences, Life 
      Sciences Center, Vilnius University, Sauletekis Av. 7, LT-10257, Vilnius, 
      Lithuania.
FAU - Talaikis, Martynas
AU  - Talaikis M
AD  - Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekis 
      Av. 7, LT-10257, Vilnius, Lithuania.
FAU - Mikoliunaite, Lina
AU  - Mikoliunaite L
AD  - Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry 
      and Geosciences, Vilnius University, Naugarduko St. 24, LT-03225, Vilnius, 
      Lithuania; Laboratory of Spectroelectrochemistry, Department of Organic 
      Chemistry, Center for Physical Sciences and Technology, Sauletekis Av. 3, 
      LT-10257, Vilnius, Lithuania.
FAU - Maneikis, Andrius
AU  - Maneikis A
AD  - Vilnius Gediminas Technical University, Sauletekis Av. 11, LT-10223, Vilnius, 
      Lithuania.
FAU - Gudiukaite, Renata
AU  - Gudiukaite R
AD  - Department of Microbiology and Biotechnology, Institute of Biosciences, Life 
      Sciences Center, Vilnius University, Sauletekis Av. 7, LT-10257, Vilnius, 
      Lithuania. Electronic address: renata.gudiukaite@gf.vu.lt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230707
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 0 (Sand)
RN  - 0 (Soil)
RN  - 059QF0KO0R (Water)
RN  - Sporosarcina pasteurii
SB  - IM
MH  - *Urease/chemistry/metabolism
MH  - *Calcium Carbonate/chemistry/metabolism
MH  - Staphylococcus/metabolism
MH  - Sand
MH  - Bacteria/metabolism
MH  - Soil
MH  - Water
OTO - NOTNLM
OT  - Atomic force microscopy
OT  - Bioconsolidation
OT  - MICP
OT  - Microbial ureases
OT  - Sporosarcina
OT  - Staphylococcus
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper
EDAT- 2023/07/10 00:41
MHDA- 2023/09/12 06:42
CRDT- 2023/07/09 19:25
PHST- 2023/04/05 00:00 [received]
PHST- 2023/06/22 00:00 [revised]
PHST- 2023/07/06 00:00 [accepted]
PHST- 2023/09/12 06:42 [medline]
PHST- 2023/07/10 00:41 [pubmed]
PHST- 2023/07/09 19:25 [entrez]
AID - S0013-9351(23)01392-0 [pii]
AID - 10.1016/j.envres.2023.116588 [doi]
PST - ppublish
SO  - Environ Res. 2023 Oct 1;234:116588. doi: 10.1016/j.envres.2023.116588. Epub 2023 
      Jul 7.