PMID- 37423401
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230828
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 140
DP  - 2023 Sep
TI  - Identification, expression pattern and functional characterization of IFN-gamma 
      involved in activating JAK-STAT pathway in Sebastes schlegeli.
PG  - 108936
LID - S1050-4648(23)00422-9 [pii]
LID - 10.1016/j.fsi.2023.108936 [doi]
AB  - IFN-gamma (interferon gamma) is a critical cytokine in the immune system involved 
      both directly and indirectly in antiviral activity, stimulation of bactericidal 
      activity, antigen presentation and activation of macrophages via the Janus 
      kinase/signal transducer and activator of transcription (JAK-STAT) pathway. The 
      IFN-gamma function is best described in cell defense against intracellular pathogens 
      in mammals, but IFN-gamma cytokine-induced metabolic change and its role in 
      anti-infection remain unknown in teleost fish. In this study, a novel IFN-gamma 
      (SsIFN-gamma) was identified from black rockfish (Sebastes schlegeli) by rapid 
      amplification of cDNA ends (RACE). The open reading frame (ORF) of SsIFN-gamma 
      encoded a putative protein of 215 amino acids and shares 60.2%-93.5% overall 
      sequence identities with other teleost IFN-gamma. SsIFN-gamma was distributed 
      ubiquitously in all the detected tissues and immune cells, which was highly 
      expressed in the spleen, gills, head kidney by quantitative real-time PCR. The 
      mRNA expression of SsIFN-gamma was significantly upregulated in the spleen, head 
      kidney, head kidney (HK) macrophages and peripheral blood lymphocytes (PBLs) 
      during pathogen infection. Meanwhile, the recombinant protein (rSsIFN-gamma) 
      exhibited an immunomodulatory function to enhance respiratory burst activity and 
      nitric oxide response of HK macrophages. Furthermore, rSsIFN-gamma could effectively 
      upregulate the expression of macrophage proinflammatory cytokine, the expression 
      of JAK-STAT signaling pathway related genes and interferon-related downstream 
      genes in the head kidney and spleen. Luciferase assays showed ISRE and GAS 
      activity were obviously enhanced after rSsIFN-gamma treatment. These results 
      indicated that SsIFN-gamma possessed apparent immunoregulatory properties and played 
      a role in fighting pathogen infection which will be helpful to further 
      understanding of the immunologic mechanism of teleosts IFN-gamma in innate immunity.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Zhang, Min
AU  - Zhang M
AD  - School of Marine Science and Engineering, Qingdao Agricultural University, 
      Qingdao, Shandong Province, 266109, China; Laboratory for Marine Biology and 
      Biotechnology, Pilot National Laboratory for Marine Science and Technology, 
      Qingdao, Shandong Province, 266109, China.
FAU - Liu, Wen-Qing
AU  - Liu WQ
AD  - School of Marine Science and Engineering, Qingdao Agricultural University, 
      Qingdao, Shandong Province, 266109, China.
FAU - Wang, Yue
AU  - Wang Y
AD  - School of Marine Science and Engineering, Qingdao Agricultural University, 
      Qingdao, Shandong Province, 266109, China.
FAU - Yan, Xue
AU  - Yan X
AD  - School of Marine Science and Engineering, Qingdao Agricultural University, 
      Qingdao, Shandong Province, 266109, China.
FAU - Wang, Bing
AU  - Wang B
AD  - School of Marine Science and Engineering, Qingdao Agricultural University, 
      Qingdao, Shandong Province, 266109, China.
FAU - Wang, Guang-Hua
AU  - Wang GH
AD  - School of Marine Science and Engineering, Qingdao Agricultural University, 
      Qingdao, Shandong Province, 266109, China. Electronic address: linda1204@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230707
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.10.2 (Janus Kinases)
RN  - 0 (STAT Transcription Factors)
RN  - 0 (Cytokines)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - *Interferon-gamma
MH  - Signal Transduction
MH  - Janus Kinases/genetics
MH  - Amino Acid Sequence
MH  - STAT Transcription Factors/genetics
MH  - *Perciformes
MH  - Cytokines/metabolism
MH  - Recombinant Proteins/genetics
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Immunoregulation
OT  - Recombinant protein
OT  - Sebastes schlegeli
OT  - SsIFN-gamma
EDAT- 2023/07/10 00:42
MHDA- 2023/08/28 06:42
CRDT- 2023/07/09 19:25
PHST- 2023/05/17 00:00 [received]
PHST- 2023/07/04 00:00 [revised]
PHST- 2023/07/06 00:00 [accepted]
PHST- 2023/08/28 06:42 [medline]
PHST- 2023/07/10 00:42 [pubmed]
PHST- 2023/07/09 19:25 [entrez]
AID - S1050-4648(23)00422-9 [pii]
AID - 10.1016/j.fsi.2023.108936 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2023 Sep;140:108936. doi: 10.1016/j.fsi.2023.108936. Epub 
      2023 Jul 7.