PMID- 37423654
OWN - NLM
STAT- MEDLINE
DCOM- 20230711
LR  - 20230718
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1273
DP  - 2023 Sep 8
TI  - Fast and bioluminescent detection of antibiotic contaminants by on-demand 
      transcription of RNA scaffold arrays.
PG  - 341538
LID - S0003-2670(23)00759-6 [pii]
LID - 10.1016/j.aca.2023.341538 [doi]
AB  - Cell-free biosensors have inspired low-cost and field-applicable methods to 
      detect antibiotic contaminants. However, the satisfactory sensitivity of current 
      cell-free biosensors is mostly achieved by sacrificing the rapidity, which 
      prolongs turnaround time by hours. Additionally, the software-based result 
      interpretation provides an obstacle for delivering these biosensors to untrained 
      individuals. Here, we present a bioluminescence-based cell-free biosensor, termed 
      enhanced Bioluminescence sensing of Ligand-Unleashed RNA Expression (eBLUE). The 
      eBLUE leveraged antibiotic-responsive transcription factors to regulate the 
      transcription of RNA arrays that can serve as scaffolds for reassembling and 
      activating multiple luciferase fragments. This process converted target 
      recognition into an amplified bioluminescence response, enabling smartphone-based 
      quantification of tetracycline and erythromycin directly in milk within 15 min. 
      Moreover, the detection threshold of eBLUE can be easily tuned according to the 
      maximum residue limits (MRLs) established by government agencies. Owing to this 
      tunable nature, the eBLUE was further repurposed as an on-demand 
      semi-quantification platform, allowing for fast ( approximately 20 min) and software-free 
      identification of safe and MRL-exceeding milk samples only by glancing over the 
      smartphone photographs. Overall, the sensitivity, rapidity and user-friendliness 
      of eBLUE demonstrate its potentials for practical applications, especially in 
      resource-limited and household settings.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Zhang, Rui
AU  - Zhang R
AD  - College of Life Sciences, South-Central Minzu University, Wuhan, 430074, PR 
      China.
FAU - Wang, Yu
AU  - Wang Y
AD  - College of Life Sciences, South-Central Minzu University, Wuhan, 430074, PR 
      China.
FAU - Deng, Haifeng
AU  - Deng H
AD  - College of Life Sciences, South-Central Minzu University, Wuhan, 430074, PR 
      China.
FAU - Zhou, Shiwen
AU  - Zhou S
AD  - College of Life Sciences, South-Central Minzu University, Wuhan, 430074, PR 
      China.
FAU - Wu, Yunhua
AU  - Wu Y
AD  - College of Life Sciences, South-Central Minzu University, Wuhan, 430074, PR 
      China.
FAU - Li, Yong
AU  - Li Y
AD  - College of Life Sciences, South-Central Minzu University, Wuhan, 430074, PR 
      China; Hubei Jiangxia Laboratory, Wuhan, 430200, PR China. Electronic address: 
      liyong081@mail.scuec.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230617
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Anti-Bacterial Agents)
RN  - 63231-63-0 (RNA)
RN  - 63937KV33D (Erythromycin)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Humans
MH  - *Anti-Bacterial Agents
MH  - RNA
MH  - Erythromycin
MH  - Luciferases
MH  - *Biosensing Techniques/methods
OTO - NOTNLM
OT  - Antibiotics
OT  - Bioluminescence
OT  - Cell-free biosensors
OT  - On-demand detection
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/07/10 00:41
MHDA- 2023/07/11 06:42
CRDT- 2023/07/09 20:55
PHST- 2023/05/12 00:00 [received]
PHST- 2023/06/11 00:00 [revised]
PHST- 2023/06/16 00:00 [accepted]
PHST- 2023/07/11 06:42 [medline]
PHST- 2023/07/10 00:41 [pubmed]
PHST- 2023/07/09 20:55 [entrez]
AID - S0003-2670(23)00759-6 [pii]
AID - 10.1016/j.aca.2023.341538 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2023 Sep 8;1273:341538. doi: 10.1016/j.aca.2023.341538. Epub 2023 
      Jun 17.