PMID- 37424778
OWN - NLM
STAT- MEDLINE
DCOM- 20230711
LR  - 20230718
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 13
DP  - 2023
TI  - Posterior concentration of Wolbachia during the early embryogenesis of the host 
      dynamically shapes the tissue tropism of Wolbachia in host Trichogramma wasps.
PG  - 1198428
LID - 10.3389/fcimb.2023.1198428 [doi]
LID - 1198428
AB  - INTRODUCTION: The bacterial endosymbiont, Wolbachia spp. induce thelytokous 
      parthenogenesis in certain parasitoid wasps, such as the egg parasitoid wasps 
      Trichogramma spp. To complete the cycle of vertical transmission, Wolbachia 
      displays efficient transovarial transmission by targeting the reproductive 
      tissues and often exhibits strong tissue-specific tropism in their host. METHOD: 
      The present study aimed to describe the basic Wolbachia distribution patterns 
      that occur during the development of Wolbachia-infected, thelytokous Trichogramma 
      dendrolimi, and T. pretiosum. We used fluorescence in situ hybridization (FISH) 
      to investigate Wolbachia signal dynamics during early embryogenesis (from 30 to 
      120 min). Wolbachia titers and distributions from the embryo to adult stages of 
      Trichogramma after early embryogenesis were detected by absolute quantitative 
      polymerase chain reaction (AQ-PCR) and FISH. The symmetry ratios (SR) of the 
      Wolbachia signals were calculated using the SR odds ratios in the anterior and 
      posterior parts of the host. The SR was determined to describe Wolbachia tropism 
      during early embryogenesis and various developmental stages of Trichogramma. 
      RESULTS: Wolbachia was concentrated in the posterior part of the embryo during 
      early embryogenesis and the various developmental stages of both T. dendrolimi 
      and T. pretiosum. Wolbachia density increased with the number of nuclei and the 
      initial mitotic division frequency during early embryogenesis. The total 
      Wolbachia titer increased with postembryogenesis development in both T. 
      dendrolimi and T. pretiosum. However, the Wolbachia densities relative to body 
      size were significantly lower at the adult and pupal stages than they were at the 
      embryonic stage. DISCUSSION: The present work revealed that posterior Wolbachia 
      concentration during early host embryogenesis determined Wolbachia localization 
      in adult wasps. By this mechanism, Wolbachia exhibits efficient vertical 
      transmission across generations by depositing only female Wolbachia-infected 
      offspring. The results of this study describe the dynamics of Wolbachia during 
      the development of their Trichogramma host. The findings of this investigation 
      helped clarify Wolbachia tropism in Trichogramma wasps.
CI  - Copyright (c) 2023 Zhou, Dong, Shang, Ning, Zhang, Wang, Che, Dong and Zhang.
FAU - Zhou, Jin-Cheng
AU  - Zhou JC
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
FAU - Dong, Qian-Jin
AU  - Dong QJ
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
FAU - Shang, Dan
AU  - Shang D
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
FAU - Ning, Su-Fang
AU  - Ning SF
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
FAU - Zhang, Huan-Huan
AU  - Zhang HH
AD  - Institute of Vegetable, Tibet Academy of Agriculture and Animal Husbandry 
      Sciences, Lhasa, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
FAU - Che, Wu-Nan
AU  - Che WN
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
FAU - Dong, Hui
AU  - Dong H
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
FAU - Zhang, Li-Sheng
AU  - Zhang LS
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230622
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
SB  - IM
MH  - Animals
MH  - Female
MH  - *Wasps/microbiology
MH  - *Wolbachia/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Embryonic Development
MH  - Parthenogenesis
PMC - PMC10324615
OTO - NOTNLM
OT  - Trichogramma dendrolimi
OT  - Trichogramma pretiosum
OT  - biological control
OT  - egg parasitoid
OT  - endosymbiont
OT  - thelytokous parthenogenesis
OT  - vertical transmission
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/07/10 06:42
MHDA- 2023/07/11 06:42
PMCR- 2023/01/01
CRDT- 2023/07/10 04:47
PHST- 2023/04/01 00:00 [received]
PHST- 2023/06/06 00:00 [accepted]
PHST- 2023/07/11 06:42 [medline]
PHST- 2023/07/10 06:42 [pubmed]
PHST- 2023/07/10 04:47 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2023.1198428 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2023 Jun 22;13:1198428. doi: 
      10.3389/fcimb.2023.1198428. eCollection 2023.