PMID- 37426761
OWN - NLM
STAT- MEDLINE
DCOM- 20230711
LR  - 20230718
IS  - 2667-2375 (Electronic)
IS  - 2667-2375 (Linking)
VI  - 3
IP  - 6
DP  - 2023 Jun 26
TI  - Unlocking the potential of microfluidics in mass spectrometry-based 
      immunopeptidomics for tumor antigen discovery.
PG  - 100511
LID - 10.1016/j.crmeth.2023.100511 [doi]
LID - 100511
AB  - The identification of tumor-specific antigens (TSAs) is critical for developing 
      effective cancer immunotherapies. Mass spectrometry (MS)-based immunopeptidomics 
      has emerged as a powerful tool for identifying TSAs as physical molecules. 
      However, current immunopeptidomics platforms face challenges in measuring 
      low-abundance TSAs in a precise, sensitive, and reproducible manner from small 
      needle-tissue biopsies (<1 mg). Inspired by recent advances in single-cell 
      proteomics, microfluidics technology offers a promising solution to these 
      limitations by providing improved isolation of human leukocyte antigen 
      (HLA)-associated peptides with higher sensitivity. In this context, we highlight 
      the challenges in sample preparation and the rationale for developing 
      microfluidics technology in immunopeptidomics. Additionally, we provide an 
      overview of promising microfluidic methods, including microchip pillar arrays, 
      valved-based systems, droplet microfluidics, and digital microfluidics, and 
      discuss the latest research on their application in MS-based immunopeptidomics 
      and single-cell proteomics.
CI  - (c) 2023 The Author(s).
FAU - Stutzmann, Charlotte
AU  - Stutzmann C
AD  - CHU Sainte Justine Research Center, Montreal, QC, Canada.
FAU - Peng, Jiaxi
AU  - Peng J
AD  - Department of Chemistry, University of Toronto, Toronto, ON, Canada.
FAU - Wu, Zhaoguan
AU  - Wu Z
AD  - CHU Sainte Justine Research Center, Montreal, QC, Canada.
FAU - Savoie, Christopher
AU  - Savoie C
AD  - CHU Sainte Justine Research Center, Montreal, QC, Canada.
FAU - Sirois, Isabelle
AU  - Sirois I
AD  - CHU Sainte Justine Research Center, Montreal, QC, Canada.
FAU - Thibault, Pierre
AU  - Thibault P
AD  - Institute for Research in Immunology and Cancer, University of Montreal, 
      Montreal, QC, Canada.
AD  - Department of Chemistry, University of Montreal, Montreal, QC, Canada.
FAU - Wheeler, Aaron R
AU  - Wheeler AR
AD  - Department of Chemistry, University of Toronto, Toronto, ON, Canada.
AD  - Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 
      Toronto, ON, Canada.
AD  - Institute of Biomaterials and Biomedical Engineering, University of Toronto, 
      Toronto, ON, Canada.
FAU - Caron, Etienne
AU  - Caron E
AD  - CHU Sainte Justine Research Center, Montreal, QC, Canada.
AD  - Department of Pathology and Cellular Biology, University of Montreal, Montreal, 
      QC, Canada.
LA  - eng
GR  - 174924/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20230626
PL  - United States
TA  - Cell Rep Methods
JT  - Cell reports methods
JID - 9918227360606676
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (HLA Antigens)
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Humans
MH  - *Microfluidics
MH  - Mass Spectrometry/methods
MH  - Histocompatibility Antigens Class I
MH  - HLA Antigens
MH  - Antigens, Neoplasm
MH  - *Neoplasms
PMC - PMC10326451
OTO - NOTNLM
OT  - HLA
OT  - MHC
OT  - immunopeptidomics
OT  - mass spectrometry
OT  - microfluidics
OT  - peptide
OT  - single-cell proteomics
COIS- The authors declare no competing interests.
EDAT- 2023/07/10 06:42
MHDA- 2023/07/11 06:42
PMCR- 2023/06/26
CRDT- 2023/07/10 05:15
PHST- 2023/07/11 06:42 [medline]
PHST- 2023/07/10 06:42 [pubmed]
PHST- 2023/07/10 05:15 [entrez]
PHST- 2023/06/26 00:00 [pmc-release]
AID - S2667-2375(23)00140-6 [pii]
AID - 100511 [pii]
AID - 10.1016/j.crmeth.2023.100511 [doi]
PST - epublish
SO  - Cell Rep Methods. 2023 Jun 26;3(6):100511. doi: 10.1016/j.crmeth.2023.100511. 
      eCollection 2023 Jun 26.