PMID- 37429064
OWN - NLM
STAT- MEDLINE
DCOM- 20230725
LR  - 20230726
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 160
DP  - 2023 Aug
TI  - Jing-Fang powder ethyl acetate extracts attenuate atopic dermatitis by modulating 
      T-cell activity.
PG  - 133-149
LID - S0161-5890(23)00136-0 [pii]
LID - 10.1016/j.molimm.2023.07.002 [doi]
AB  - Jing-Fang powder ethyl acetate extract (JFEE) and its isolated C (JFEE-C) possess 
      favorable anti-inflammatory and anti-allergic properties; however, their 
      inhibitory effects on T cell activity remain unknown. In vitro, Jurkat T cells 
      and primary mouse CD4(+) T cells were used to explore the regulatory effects of 
      JFEE and JFEE-C as well as their potential mechanisms on activated T cells. 
      Furthermore, T cell-mediated atopic dermatitis (AD) mouse model was established 
      to confirm these inhibitory effects in vivo. The results showed that JFEE and 
      JFEE-C inhibited T cell activation by suppressing the production of interleukin-2 
      (IL-2) and interferon-gamma (IFN-gamma) without showing cytotoxicity. Flow cytometry 
      showed the inhibitory effects of JFEE and JFEE-C on the activation-induced 
      proliferation and apoptosis of T cells. Pretreatment with JFEE and JFEE-C also 
      decreased the expression levels of several surface molecules, including CD69, 
      CD25, and CD40L. Moreover, it was confirmed that JFEE and JFEE-C inhibited T cell 
      activation by downregulating the TGF-beta-activated kinase 1 (TAK1)/nuclear 
      kappa-light-chain-enhancer of activated B cells (NF-kappaB)/mitogen-activated protein 
      kinase (MAPK) signaling pathways. The combination of these extracts with C25-140 
      intensified the inhibitory effects on IL-2 production and p65 phosphorylation. 
      The oral administration of JFEE and JFEE-C notably weakened AD manifestations, 
      including the infiltration of mast cells and CD4(+) cells, epidermis and dermis 
      thicknesses, serum levels of immunoglobulin E (IgE) and thymic stromal 
      lymphopoietin (TSLP), and gene expression levels of T helper (Th) cells-related 
      cytokines in vivo. The underlying mechanisms of the inhibitory effects of JFEE 
      and JFEE-C on AD were related to attenuating T cell activity through NF-kappaB/MAPK 
      pathways. In conclusion, this study suggested that JFEE and JFEE-C exhibited 
      anti-atopic efficacy by attenuating T cell activity and might possess a curative 
      potential for T cell-mediated diseases.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Zhao, Ge
AU  - Zhao G
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 
      611137, PR China.
FAU - Tong, Yue
AU  - Tong Y
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 
      611137, PR China.
FAU - Xu, Jie
AU  - Xu J
AD  - College of Material and Chemical Engineering, Chuzhou University, Chuzhou, Anhui 
      239000, PR China.
FAU - Zhu, Wenjing
AU  - Zhu W
AD  - Department of Pharmacy, Affiliated Hospital of Southwest Medical University, 
      Luzhou, Sichuan 646000, PR China.
FAU - Zeng, Jiuseng
AU  - Zeng J
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 
      611137, PR China.
FAU - Liu, Rong
AU  - Liu R
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 
      611137, PR China.
FAU - Luan, Fei
AU  - Luan F
AD  - Department of Pharmaceutics, The Key Laboratory of Basic and New Drug Research of 
      Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang, 
      Shaanxi 712046, PR China. Electronic address: luanfeiren@163.com.
FAU - Zeng, Nan
AU  - Zeng N
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 
      611137, PR China. Electronic address: 19932015@cdutcm.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230708
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Interleukin-2)
RN  - 76845O8NMZ (ethyl acetate)
RN  - 0 (Powders)
RN  - 0 (NF-kappa B)
RN  - 0 (Cytokines)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Dermatitis, Atopic/drug therapy/chemically induced
MH  - Interleukin-2
MH  - Powders/adverse effects/metabolism
MH  - NF-kappa B/metabolism
MH  - Cytokines/metabolism
MH  - CD4-Positive T-Lymphocytes/metabolism
MH  - Mice, Inbred BALB C
MH  - Plant Extracts/pharmacology
OTO - NOTNLM
OT  - Atopic dermatitis
OT  - IL-2
OT  - Jing-Fang powder ethyl acetate extracts
OT  - NF-kappaB/MAPK pathways
OT  - T cell activation
COIS- Declaration of Competing Interest The authors declare no conflicts of interest.
EDAT- 2023/07/10 19:08
MHDA- 2023/07/25 06:42
CRDT- 2023/07/10 18:00
PHST- 2023/04/14 00:00 [received]
PHST- 2023/06/24 00:00 [revised]
PHST- 2023/07/02 00:00 [accepted]
PHST- 2023/07/25 06:42 [medline]
PHST- 2023/07/10 19:08 [pubmed]
PHST- 2023/07/10 18:00 [entrez]
AID - S0161-5890(23)00136-0 [pii]
AID - 10.1016/j.molimm.2023.07.002 [doi]
PST - ppublish
SO  - Mol Immunol. 2023 Aug;160:133-149. doi: 10.1016/j.molimm.2023.07.002. Epub 2023 
      Jul 8.