PMID- 37429607
OWN - NLM
STAT- MEDLINE
DCOM- 20231129
LR  - 20231129
IS  - 1742-481X (Electronic)
IS  - 1742-4801 (Print)
IS  - 1742-4801 (Linking)
VI  - 20
IP  - 10
DP  - 2023 Dec
TI  - Human umbilical cord mesenchymal stem cell-derived exosomes combined with gelatin 
      methacryloyl hydrogel to promote fractional laser injury wound healing.
PG  - 4040-4049
LID - 10.1111/iwj.14295 [doi]
AB  - To investigate whether human umbilical cord mesenchymal stem cell-derived 
      exosomes combined with gelatin methacryloyl (GelMA) hydrogel are beneficial in 
      promoting healing of laser-injured skin wounds in mice. Supernatants of cultured 
      human umbilical cord mesenchymal stem cells (HUC-MSCs) were collected to obtain 
      human umbilical cord MSC-derived exosomes (HUC-MSCs-Exos), which were combined 
      with GelMA hydrogel complex to treat a mouse fractional laser injury model. The 
      study was divided into PBS group, EX (HUC-MSCs-Exos) group, GEL (GelMA hydrogel) 
      group and EX+GEL (HUC-MSCs-Exos combined with GelMA hydrogel) group. The healing 
      of laser-injured skin in each group was observed by gross view and dermatoscopy, 
      and changes in skin structure, angiogenesis and proliferation-related indexes 
      were observed during the healing process of laser-injured skin in each group. The 
      results of the animal experiments showed that the EX and GEL groups alone and the 
      EL+EX group exhibited less inflammatory response compared to the PBS group. The 
      EX and GEL groups showed marked tissue proliferation and favourable angiogenesis, 
      which promoted the wound healing well. The GEL+EX group had the most significant 
      promotion of wound healing compared to the PBS group. qPCR results showed that 
      the expression levels of proliferation-related factors, including KI67 and VEGF 
      and angiogenesis-related factor CD31, were significantly higher in the GEL+EX 
      group than in the other groups, with a time-dependent effect. The combination of 
      HUC-MSCs-Exos and GelMA hydrogel is beneficial in reducing the early inflammatory 
      response of laser-injured skin in mice and promoting its proliferation and 
      angiogenesis, which in turn promotes wound healing.
CI  - (c) 2023 The Authors. International Wound Journal published by Medicalhelplines.com 
      Inc and John Wiley & Sons Ltd.
FAU - Zhang, Xinling
AU  - Zhang X
AD  - Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
FAU - Ding, Pengbing
AU  - Ding P
AD  - Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
FAU - Chen, Yujie
AU  - Chen Y
AD  - Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
FAU - Lin, Zhiyu
AU  - Lin Z
AD  - Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
FAU - Zhao, Xun
AU  - Zhao X
AD  - Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
FAU - Xie, Hongbin
AU  - Xie H
AUID- ORCID: 0009-0003-3723-1814
AD  - Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
LA  - eng
GR  - H65469-05/Beijing Regenerative Biotechnology Research Institute/
PT  - Journal Article
DEP - 20230710
PL  - England
TA  - Int Wound J
JT  - International wound journal
JID - 101230907
RN  - 0 (Hydrogels)
RN  - 0 (gelatin methacryloyl)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - Hydrogels/therapeutic use
MH  - *Exosomes/metabolism
MH  - Wound Healing/physiology
MH  - Disease Models, Animal
MH  - Umbilical Cord
MH  - *Mesenchymal Stem Cells
PMC - PMC10681517
OTO - NOTNLM
OT  - exosomes
OT  - fractional laser injury wound
OT  - gelatin methacryloyl hydrogel
OT  - mesenchymal stem cells
OT  - umbilical cord
COIS- The authors declare no competing interests.
EDAT- 2023/07/11 01:08
MHDA- 2023/11/29 06:42
PMCR- 2023/07/10
CRDT- 2023/07/10 20:33
PHST- 2023/06/08 00:00 [revised]
PHST- 2023/04/27 00:00 [received]
PHST- 2023/06/16 00:00 [accepted]
PHST- 2023/11/29 06:42 [medline]
PHST- 2023/07/11 01:08 [pubmed]
PHST- 2023/07/10 20:33 [entrez]
PHST- 2023/07/10 00:00 [pmc-release]
AID - IWJ14295 [pii]
AID - 10.1111/iwj.14295 [doi]
PST - ppublish
SO  - Int Wound J. 2023 Dec;20(10):4040-4049. doi: 10.1111/iwj.14295. Epub 2023 Jul 10.