PMID- 37434200
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230718
IS  - 1750-9378 (Print)
IS  - 1750-9378 (Electronic)
IS  - 1750-9378 (Linking)
VI  - 18
IP  - 1
DP  - 2023 Jul 11
TI  - p16(INK4a) and pRb expression in laryngeal squamous cell carcinoma with and 
      without infection by EBV or different genotypes of HPV: a retrospective study.
PG  - 43
LID - 10.1186/s13027-023-00514-x [doi]
LID - 43
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) represents one of the 
      principal tumors of the head and neck. Human papillomavirus (HPV) and 
      Epstein-Barr virus (EBV) are considered risk factors for the development and the 
      clinical prognosis of LSCC. High levels of p16(INK4a) are suggested as a 
      surrogate marker of HPV or EBV infection in some head and neck tumors but in LSCC 
      is still controversial. Furthermore, pRb expression may be considered an 
      additional biomarker but it has not been clearly defined. This work aimed to 
      compare the expression of pRb and p16(INK4a) as possible biomarkers in tumor 
      tissues with and without infection by EBV or different genotypes of HPV from 
      patients with LSCC. METHODS: Tumor samples from 103 patients with LSCC were 
      previously investigated for the presence and genotypes of HPV using the INNO-LiPA 
      line probe assay and for the infection of EBV by qPCR. p16 (INK4a) and pRb 
      expression was assessed by immunohistochemistry. RESULTS: Of the 103 tumor 
      samples, expression of p16(INK4a) was positive in 55 (53.4%) and of this, 32 
      (56.1%) were positive for HPV whereas 11 (39.3%) were EBV positive but both 
      without a significantly difference (p > 0.05). pRb expression was positive in 78 
      (75.7%) and a higher frequency of this expression was observed in HPV negative 
      samples (87.0%) (p = 0.021) and in high-risk HPV negative samples (85.2%) 
      (p = 0.010). No difference was observed when comparing pRb expression and EBV 
      infection status (p > 0.05). CONCLUSION: Our results support the suggestion that 
      p16(INK4a) is not a reliable surrogate marker for identifying HPV or EBV 
      infection in LSCC. On the other hand, most of our samples had pRb expression, 
      which was more frequent in tumors without HPV, suggesting that pRb could indicate 
      HPV negativity. However, more studies with a larger number of cases are required, 
      including controls without LSCC and evaluating other molecular markers to 
      determine the real role of p16(INK4a) and pRb in LSCC.
CI  - (c) 2023. The Author(s).
FAU - Vazquez-Guillen, Jose Manuel
AU  - Vazquez-Guillen JM
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Palacios-Saucedo, Gerardo C
AU  - Palacios-Saucedo GC
AD  - Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de 
      Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de 
      Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo 
      Leon, Mexico. palsaugc@gmail.com.
FAU - Alanis-Valdez, Alondra Yamileth
AU  - Alanis-Valdez AY
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Huerta-Escobedo, Andrea
AU  - Huerta-Escobedo A
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Zavala-Pompa, Angel
AU  - Zavala-Pompa A
AD  - Laboratorio Medicina Diagnostica S.A. de C.V., Monterrey, Nuevo Leon, Mexico.
FAU - Rivera-Morales, Lydia Guadalupe
AU  - Rivera-Morales LG
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Martinez-Torres, Ana Carolina
AU  - Martinez-Torres AC
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Gonzalez-Villasana, Vianey
AU  - Gonzalez-Villasana V
AD  - Departamento de Biologia Celular y Genetica, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Serna-Hernandez, Julio Cesar
AU  - Serna-Hernandez JC
AD  - Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de 
      Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de 
      Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo 
      Leon, Mexico.
FAU - Hernandez-Martinez, Silvia Judith
AU  - Hernandez-Martinez SJ
AD  - Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de 
      Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de 
      Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo 
      Leon, Mexico.
FAU - Castelan-Maldonado, Edmundo Erbey
AU  - Castelan-Maldonado EE
AD  - Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de 
      Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de 
      Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo 
      Leon, Mexico.
FAU - Montalvo-Banuelos, Martha Socorro
AU  - Montalvo-Banuelos MS
AD  - Departamento de Foniatria, Hospital General de Zona No. 6, Instituto Mexicano del 
      Seguro Social, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Alonso-Tellez, Cesar Alejandro
AU  - Alonso-Tellez CA
AD  - Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de 
      Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de 
      Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo 
      Leon, Mexico.
FAU - Sanchez-Fresno, Ethel Corinthia
AU  - Sanchez-Fresno EC
AD  - Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de 
      Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de 
      Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo 
      Leon, Mexico.
FAU - Tamez-Guerra, Reyes S
AU  - Tamez-Guerra RS
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
FAU - Rodriguez-Padilla, Cristina
AU  - Rodriguez-Padilla C
AD  - Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, 
      Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20230711
PL  - England
TA  - Infect Agent Cancer
JT  - Infectious agents and cancer
JID - 101276559
PMC - PMC10334558
OTO - NOTNLM
OT  - Epstein-Barr virus
OT  - Human papillomavirus
OT  - Laryngeal squamous cell carcinoma
OT  - p16 INK4a
OT  - pRb
COIS- The authors declare there are no competing interests.
EDAT- 2023/07/12 01:07
MHDA- 2023/07/12 01:08
PMCR- 2023/07/11
CRDT- 2023/07/11 23:43
PHST- 2022/06/14 00:00 [received]
PHST- 2023/05/24 00:00 [accepted]
PHST- 2023/07/12 01:08 [medline]
PHST- 2023/07/12 01:07 [pubmed]
PHST- 2023/07/11 23:43 [entrez]
PHST- 2023/07/11 00:00 [pmc-release]
AID - 10.1186/s13027-023-00514-x [pii]
AID - 514 [pii]
AID - 10.1186/s13027-023-00514-x [doi]
PST - epublish
SO  - Infect Agent Cancer. 2023 Jul 11;18(1):43. doi: 10.1186/s13027-023-00514-x.