PMID- 37434456
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230718
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 25
IP  - 6
DP  - 2023 Jun 28
TI  - Peroxiredoxin 3 Inhibits Cardiac Fibrosis in Mice via NOX4-P38 Signalling.
PG  - 391-398
LID - 704470 [pii]
LID - 10.22074/cellj.2023.557603.1082 [doi]
AB  - OBJECTIVE: Peroxiredoxin-3 (Prx-3) is widely acknowledged as an antioxidant that 
      protects against mitochondrial reactive oxygen species. Nonetheless, its role in 
      cardiac fibrosis has not been elucidated. We aim to explore the role and 
      mechanism of Prx-3 in cardiac fibrosis. MATERIALS AND METHODS: In this 
      experimental study, mice received subcutaneous injections of isoproterenol (ISO) 
      for 14 consecutive days (10 mg/kg/d for three days, followed by 5 mg/kg/d for 11 
      days) to establish a cardiac fibrosis model. The mice were subsequently injected 
      with adenovirus-Prx-3 (ad-Prx-3) to enable Prx-3 overexpression. Echocardiography 
      was used to evaluate cardiac function. Mice heart fibroblasts were isolated and 
      stimulated with transforming growth factor beta1 (TGFbeta1) to induce fibrosis in 
      vitro. Cells were also transfected with ad-Prx-3 for overexpression of Prx-3. 
      RESULTS: Echocardiographic diameters and fibrosis markers indicated that Prx-3 
      could inhibit ISO-induced cardiac dysfunction and fibrosis. Fibroblasts with 
      Prx-3 overexpression exhibited reduced activation, proliferation, and collagen 
      transcription. We found that Prx-3 reduced the expression of NADPH oxidase 4 
      (NOX4) and reduced P38 levels. After treatment with a P38 inhibitor, the Prx-3 
      overexpression-induced anti-fibrosis effect was mitigated. CONCLUSION: Prx-3 
      could protect against ISO-induced cardiac fibrosis by inhibiting the NOX4-P38 
      pathway.
FAU - Xiong, Xiaoqi
AU  - Xiong X
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Li, Jun
AU  - Li J
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Chen, Zhen
AU  - Chen Z
AD  - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan City, Hubei Province, P.R. 
      China.
FAU - Luo, Changjun
AU  - Luo C
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Wei, Wei
AU  - Wei W
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Li, Bing
AU  - Li B
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Kang, Yi
AU  - Kang Y
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Nong, Xiuhong
AU  - Nong X
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China.
FAU - Ai, Fen
AU  - Ai F
AD  - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan City, Hubei Province, P.R. 
      China. Email: wtt20220520@126.com.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Cardiology, Liuzhou Municipal Liutie Central Hospital, Liuzhou 
      City, Guang Xi Province, P.R. China. Email: abc20220427@126.com.
LA  - eng
PT  - Journal Article
DEP - 20230628
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC10331444
OTO - NOTNLM
OT  - Cardiac fibrosis
OT  - Isoproterenol
OT  - NADPH Oxidase 4
OT  - P38
OT  - Peroxiredoxin 3
EDAT- 2023/07/12 06:42
MHDA- 2023/07/12 06:43
PMCR- 2023/06/01
CRDT- 2023/07/12 02:43
PHST- 2022/07/11 00:00 [received]
PHST- 2023/07/12 06:43 [medline]
PHST- 2023/07/12 06:42 [pubmed]
PHST- 2023/07/12 02:43 [entrez]
PHST- 2023/06/01 00:00 [pmc-release]
AID - 704470 [pii]
AID - 10.22074/cellj.2023.557603.1082 [doi]
PST - epublish
SO  - Cell J. 2023 Jun 28;25(6):391-398. doi: 10.22074/cellj.2023.557603.1082.